Production, purification and analysis of the isomalto-oligosaccharides from Chinese chestnut (Castanea mollissima Blume) and the prebiotics effects of them on proliferation of Lactobacillus

Synthesis of Isomaltooligosaccharides (IMOs) from Sweet Potato Starch by Simultaneous Saccharification and Transglycosylation Using Saccharomyces cerevisiae Var. diastaticus BE 134 to Improve Purity of IMOs

“…ese results were because the pullulanase enhanced hydrolysis of α-1,6linkages in starch molecules [28]. In particular, the combination between pullulanase and β-amylase at a suitable ratio in the same SST reaction condition proved the promoting generation of more short chain maltose and maltodextrin which resulted in improving the synthesis performance of IMOs [1,7]. Besides, the HPLC analysis data also showed that application of the pullulanase for treatment of IMOs product had no negligible effects on the quality of IMOs.…”

“…e most abundant and well-recognized functional components in the IMOs are isomaltose/α-D-Glcp-(1 ⟶ 6)-α-D-Glcp (IG 2 ), isomaltotriose/α-D-Glcp-(1 ⟶ 6)-α-D-Glcp-(1 ⟶ 6)-D-Glcp (IG 3 ), and isomaltotetraose/α-D-Glcp-(1⟶6)-(α-D-Glcp-(1⟶6)) 2 -D-Glcp (IG 4 ), which have been collectively abbreviated as IG 234 [3]. e recent studies reported that the main constituents found in commercial IMOs consisted of isomaltose (IG 2 ), panose, isomaltotriose (IG 3 ), and isomaltotetraose (IG 4 ) [7]. is may be related to the ability of transglucosidase enzyme derived from Aspergillus niger mainly existent on short-chain oligosaccharide substrates [1,8].…”

“…Specifically, Niu et al [4] calculated the formed IMOs concentration synthesizing from enzyme cocktail based on obtained sum of concentrations of all IG 2 , IG 3 , and panose. Also, Cui et al [7] also computed IMOs concentration extracted from Chinese chestnut starch by total concentrations of IG 2 , IG 3 , and panose. e IMOs total concentration obtained from banana flour was sum of both IG 3 and IG 4 [9].…”

“…en, in the transglycosidation step, transglucosidase usually carries out two tasks: (1) the hydrolysis of α-D-glucooligosaccharides and (2) transfer of the glucosyl group to 6-OH of glucose. At the end of process, beside the desirable products of isomaltose and panose which are formed from the transglycosidation of glucosyl to D-glucose and maltose, respectively, the process also generates a large amount of glucose and α- (1,4) oligomers (mainly maltose) as byproducts [7,19]. ese impure by-products need to be removed from obtained IMO products.…”

“…Due to possessing many advantages, the studies to produce IMOs from various sources have gained increasing attention by scholars. Beside the IMOs that have been found in traditional products such as honey [29], miso [30], and sake [31], the IMOs have been produced from a wide variety of starch sources such as tapioca starch [25,32], corn starch [4], rice starch [1], banana starch [9], sugarcane plants [33], and chestnut starch [7].…”