Production, purification, and characterization of a novel cold-active superoxide dismutase from the Antarctic strain Aspergillus glaucus 363

Abrashev, Radoslav; Feller, Georges; Kostadinova, Nedelina; Krumova, Ekaterina; Alexieva, Zlatka; Gerginova, Maria; Spasova, Boryana; Miteva-Staleva, Jeni; Vassilev, Spassen V.; Angelova, Maria

doi:10.1016/j.funbio.2016.03.002

Cited by 22 publications

(20 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…High concentrations of superoxide dismutase were produced by the Antarctic fungus Aspergillus glaucus 363 following cold stress at 10.0 C using a nitro-blue tetrazolium substrate [84]. Tosi et al [126] reported catalase production by fungal strains recovered from soil samples belonging to the genera Aspergillus, Cladosporium, and Penicillium.…”

Section: Superoxide Dismutases and Catalasesmentioning

confidence: 99%

“…Cold-adapted antioxidant enzymes, such as superoxide dismutases and catalases can be very important for the prevention of skin injury when used in cosmetic formulations. These enzymes can also be used in medicine, as a pulp-capping agent, as an inhibitor of pulmonary vascular oxidative stress, and in the treatment of septic shock [84,126].…”

Section: Superoxide Dismutases and Catalasesmentioning

confidence: 99%

“…In studies carried out by Lario et al [81], Petrescu et al [98], and Ray et al [80], ion exchange chromatography was performed. On the other hand, in the studies reported by Abrashev et al [84], De Mot and Verachtert [63], Florczak et al [72], Gao et al [64], Turkiewicz et al [107], and Yu et al [79] size exclusion with ion exchange and/or affinity chromatography were combined.…”

Section: Purification Of Cold-adapted Enzymes From Antarctic Fungimentioning

confidence: 99%

See 2 more Smart Citations

Cold-adapted enzymes produced by fungi from terrestrial and marine Antarctic environments

Duarte

Santos

Vianna

et al. 2017

Critical Reviews in Biotechnology

114

View full text Add to dashboard Cite

Antarctica is the coldest, windiest, and driest continent on Earth. In this sense, microorganisms that inhabit Antarctica environments have to be adapted to harsh conditions. Fungal strains affiliated with Ascomycota and Basidiomycota phyla have been recovered from terrestrial and marine Antarctic samples. They have been used for the bioprospecting of molecules, such as enzymes. Many reports have shown that these microorganisms produce cold-adapted enzymes at low or mild temperatures, including hydrolases (e.g. α-amylase, cellulase, chitinase, glucosidase, invertase, lipase, pectinase, phytase, protease, subtilase, tannase, and xylanase) and oxidoreductases (laccase and superoxide dismutase). Most of these enzymes are extracellular and their production in the laboratory has been carried out mainly under submerged culture conditions. Several studies showed that the cold-adapted enzymes exhibit a wide range in optimal pH (1.0-9.0) and temperature (10.0-70.0 °C). A myriad of methods have been applied for cold-adapted enzyme purification, resulting in purification factors and yields ranging from 1.70 to 1568.00-fold and 0.60 to 86.20%, respectively. Additionally, some fungal cold-adapted enzymes have been cloned and expressed in host organisms. Considering the enzyme-producing ability of microorganisms and the properties of cold-adapted enzymes, fungi recovered from Antarctic environments could be a prolific genetic resource for biotechnological processes (industrial and environmental) carried out at low or mild temperatures.

show abstract

Section: Superoxide Dismutases and Catalasesmentioning

confidence: 99%

Section: Superoxide Dismutases and Catalasesmentioning

confidence: 99%

Section: Purification Of Cold-adapted Enzymes From Antarctic Fungimentioning

confidence: 99%

See 1 more Smart Citation

Cold-adapted enzymes produced by fungi from terrestrial and marine Antarctic environments

Duarte

Santos

Vianna

et al. 2017

Critical Reviews in Biotechnology

114

View full text Add to dashboard Cite

show abstract

“…Ni‐SOD is relatively little research, and only found in Streptomyces and cyanobacteria . SODs, as superoxide anion radical scavengers, have been widely applied in the medical, agricultural, food, and cosmetics industry .…”

Section: Introductionmentioning

confidence: 99%

“…Raychaudhuri et al firstly reported the presence of cytosolic Fe‐SOD in yeast Rhodotorula glutinis . Untill now, Fe‐SOD genes had been detected in several yeasts and fungi, such as Rhodosporidium toruloides CECT1137 , Rhodotorula toruloides NP11 , Aspergillus glaucus 363 .…”

Section: Introductionmentioning

confidence: 99%

Cloning and characterization of iron‐superoxide dismutase in Antarctic yeast strain Rhodotorula mucilaginosa AN5

et al. 2017

View full text Add to dashboard Cite

A novel superoxide dismutase gene from Antarctic yeast Rhodotorula mucilaginosa AN5 was cloned, sequenced, and then expressed in Escherichia coli. The R. mucilaginosa AN5 SOD (RmFeSOD) gene was 639 bp open reading frame in length, which encoded a protein of 212 amino acids with a deduced molecular mass of 23.5 kDa and a pI of 7.89. RmFeSOD was identified as iron SOD type with a natural status of homodimer. The recombinant RmFeSOD showed good pH stability in the pH 1.0-9.0 after 1 h incubation. Meanwhile, it was found to behave relatively high thermostability, and maintained more than 80% activity at 50 °C for 1 h. By addition of 1 mM metal ions, the enzyme activity increased by Zn , Cu , Mn , and Fe , and inhibited only by Mg . RmFeSOD showed relatively low tolerance to some compounds, such as PMSF, SDS, Tween-80, Triton X-100, DMSO, β-ME, and urea. However, DTT showed no inhibition to enzyme activity. Using copper stress experiment, the RmFeSOD recombinant E. coli exhibited better growth than non-recombinant bacteria, which revealed that RmFeSOD might play an important role in the adaptability of heavy metals.

show abstract

Cold‐active catalase from the psychrotolerant fungus Penicillium griseofulvum

et al. 2021

Self Cite

View full text Add to dashboard Cite

Cold-active catalase (CAT) elicits great interest because of its vast prospective at the medical, commercial, and biotechnological levels. The study paper reports the production of cold-active CAT by the strain Penicillium griseofulvum P29 isolated from Antarctic soil. Improved enzyme production was achieved by optimization of medium and culture conditions. Maximum CAT was demonstrated under low glucose content (2%), 10% inoculum size, temperature 20°C, and dissolved oxygen concentration (DO) 40%. An effective laboratory technology based on changing the oxidative stress level through an increase of DO in the bioreactor was developed. The used strategy resulted in a 1.7-and 1.4-fold enhanced total enzyme activity and maximum enzyme productivity. The enzyme was purified and characterized. P. griseofulvum P29 CAT was most active at approximately 20°C and pH 6.0. Its thermostability was in the range between 5°C and 40°C.

show abstract

Production, purification, and characterization of a novel cold-active superoxide dismutase from the Antarctic strain Aspergillus glaucus 363

Cited by 22 publications

References 57 publications

Cold-adapted enzymes produced by fungi from terrestrial and marine Antarctic environments

Cold-adapted enzymes produced by fungi from terrestrial and marine Antarctic environments

Cloning and characterization of iron‐superoxide dismutase in Antarctic yeast strain Rhodotorula mucilaginosa AN5

Cold‐active catalase from the psychrotolerant fungus Penicillium griseofulvum

Contact Info

Product

Resources

About