Production, purification, and characterization of lipase from thermophilic and alkaliphilic Bacillus coagulans BTS-3

Bacillus smithii BTMS 11, isolated from marine sediment, produced alkaline and thermostable lipase. The enzyme was purified to homogeneity by ammonium sulfate precipitation and ion exchange chromatography which resulted in 0.51 % final yield and a 4.33 fold of purification. The purified enzyme was found to have a specific activity of 360 IU/mg protein. SDS-PAGE analyses, under non-reducing and reducing conditions, yielded a single band of 45 kDa indicating the single polypeptide nature of the enzyme and zymogram analysis using methylumbelliferyl butyrate as substrate confirmed the lipolytic activity of the protein band. The enzyme was found to have 50°C and pH 8.0 as optimum conditions for maximal activity. However, the enzyme was active over wide range of temperatures (30-80°C) and pH (7.0-10.0). Effect of a number of metal salts, solvents, surfactants, and other typical enzyme inhibitors on lipase activity was studied to determine the novel characteristics of the enzyme. More than 90 % of the enzyme activity was observed even after 3 h of incubation in the presence of commercial detergents Surf, Sunlight, Ariel, Henko, Tide and Ujala indicating the detergent compatibility of B. smithii lipase. The enzyme was also found to be efficient in stain removal from cotton cloths. Further it was observed that the enzyme could catalyse ester synthesis between fatty acids of varying carbon chain lengths and methanol with high preference for medium to long chain fatty acids showing 70 % of esterification. Results of the study indicated scope for application of this marine bacterial lipase in various industries.

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“…, and Zn 2? (Kumar et al 2005). The enhanced catalytic activity of B. smithii lipase observed in the presence of Co 2?…”

Section: Discussionmentioning

confidence: 97%

Detergent compatible alkaline lipase produced by marine Bacillus smithii BTMS 11

Lailaja

Chandrasekaran

2013

World J Microbiol Biotechnol

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“…Lipase from B. subtilis I-4 was activated by Ca 2+ , while Mg 2+ , Zn 2+ , and Cu 2+ ions inhibited its activity (Fig. 2c) and Mg 2+ ions enhanced the enzyme activity; Na + ions had no effect on enzyme activity (Kumar et al 2005). Mukeshkumar et al (2012) reported that Fe 2+ , Mg 2+ , Triton X-100, and Tween esters enhanced lipase production in Bacillus sp.…”

Section: Effect Of Ph and Temperature On Activity And Stability Of LImentioning

confidence: 93%

“…The apparent molecular masses of LipA and LipB determined by the SDS-PAGE were 50 and 57 kDa, respectively (Salameh and Wiegel 2007). Kumar et al (2005) reported the molecular weight of lipase 31 kDa on the SDS-PAGE for B. coagulans BTS-3. Kumar et al (2012) reported a single band of 62.2 kDa lipase from B. pumilus RK31 on SDS-PAGE.…”

Section: Sds-pagementioning

confidence: 99%

Characterization of Lipase from Bacillus subtilisI-4 and Its Potential Use in Oil Contaminated Wastewater

Iqbal

Rehman

2015

Braz. arch. biol. technol.

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“…The maximum lipolytic activity was 12 U/ml in the presence of olive oil by P. aurantiogriseum [38]. A. niger produced lipase in a lipid free medium but required an inducer for improved production [39]. Stoytcheva et al, (2010) recorded that the maximum lipolytic activity was 12 U/ml in the presence of olive oil by P. aurantiogriseum.…”

Section: Effect Of Lipid Carbon Sources and Glucosementioning

confidence: 99%

Effect of Environmental and Nutritional Parameters on the Extracellular Lipase Production by <i>Aspergillus niger</i>

El-Batal

Farrag

Elsayed

et al. 2016

ILNS

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Abstract. The present investigation was carried out to evaluate the effect of different growth conditions on lipase production by Aspegillus niger. The extracellular lipase producing fungus was isolated from spent bleaching earths. Optimizations of physical and chemical parameters were done for maximum lipase production using this isolate. Growth of the organism and lipase production were measured usig varying pH (4 -9), incubation temperature (20 -30 °C), incubation time (8 -80 hrs.), carbon sources, nitrogen sources, and shaking speed. Enhanced lipase production was observed at 24 °C, pH 7 and after 72hrs of incubation. Olive oil 5 % was observed as the most effective carbon source and Yeast extract 1.0 % as the most effective nitrogen source for lipase production. The optimum shaking value to get maximum lipase activity by Aspergillus niger was 200 rpm.

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Production, purification, and characterization of lipase from thermophilic and alkaliphilic Bacillus coagulans BTS-3

Cited by 213 publications

References 29 publications

Detergent compatible alkaline lipase produced by marine Bacillus smithii BTMS 11

Detergent compatible alkaline lipase produced by marine Bacillus smithii BTMS 11

Characterization of Lipase from Bacillus subtilisI-4 and Its Potential Use in Oil Contaminated Wastewater

Effect of Environmental and Nutritional Parameters on the Extracellular Lipase Production by <i>Aspergillus niger</i>

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