Production, Purification, and Characterization of Bioactive Metabolites Produced From Rare Actinobacteria Pseudonocardia Alni

Omran, Rabab; Kadhem, Mohammed Fadhil

doi:10.22159/ajpcr.2016.v9s3.14961

Cited by 10 publications

(4 citation statements)

References 18 publications

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“…Однак, за останні 20 років повторне відкриття раніше охарактеризованих біоактивних сполук і надлишковість штамів знизили інтерес до ґрунтових стрептоміцетів, як до джерела нових біоактивних сполук [11,13]. Актинобактерії, мешканці морського середовища (морських відкладень, коралових рифів, безхребетних тощо), набули цінності завдяки своєму хеморізноманіттю, включаючи продукцію численних біоактивних вторинних метаболітів [10,17], що залежить від їх середовища з екстремальними коливаннями тиску, солоності, світла і температури [14]. Було показано, що морські актинобактерії демонструють більш різноманітні та кращі властивості порівняно з наземними актинобактеріями щодо антибактеріальних, антигрибкових, антибіоплівкових, антикоагулянтних та противірусних ефектів [13,24].…”

Section: антагоністична активність чорноморських стрептоміцетів виділ...unclassified

Antagonistic Properties of the Black Sea Streptomycetes Isolated From the Fouling of Shell Rock and Mussels

Страшнова¹,

Potapenko²,

Korotaeva³

et al. 2023

mbt

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SummaryThe rapid emergence of resistance by bacterial and fungal pathogens is a serious problem in the health care system, which causes the search for new promising producers of antimicrobial natural products in various ecological niches. Aim. To determine the antagonistic activity of streptomycetes isolated from the biological fouling of natural shell rock and mussels of the Odesa gulf of the Black Sea. Methods. The antagonistic activity of 19 and 14 strains of streptomycetes isolated from the fouling of shell rock and mussels of the Odesa gulf, respectively, were investigated. Streptomycetes were pre-cultivated on agar media Gause 1, Gause 2 and oat agar with sea salt (2%) at a temperature of 30 °C for 10 days. Antagonistic activity against 12 test cultures was determined by the block method. Results. All isolated marine streptomycetes are antagonists of at least one strain of the indicator microorganism. Antibiotic activity depended on the source of the streptomycetes isolation, culture medium and properties of specific strains of both producers and test cultures. The best activity of streptomycetes strains from shell rock was shown after cultivation on Gause 1 medium, and streptomycetes from mussels – after cultivation on Gause 2 medium. The zones of no growth of sensitive indicators ranged from 12,4±0,3 mm to 20,6±0,2 mm (under the influence of streptomycetes from shell rock) and from 12,4±0,2 mm to 39,7±0,2 mm (under the influence of streptomycetes from mussels). Streptomyces sp. Lim 2.2 (strain from a shell rock) inhibited the growth of 8 test cultures, and strains from mussels Streptomyces sp. Myt 4b and Myt 7ch – 10 test cultures. Indicator strains of gram-positive bacteria were the most sensitive to all streptomycetes, in particular, strain Staphylococcus aureus ATCC 25923 was most inhibited by metabolites of Streptomyces spp. Myt 12a and Myt 12b. Conclusions. Antagonistic activity of streptomycetes isolated from the Black Sea depended on the source of isolation, pre-cultivation medium and properties of both producer strains and indicator microorganisms. The greatest activity of streptomycete strains from shell rock and mussels was shown after preliminary cultivation, respectively, on Gause 1 and Gause 2 media against gram-positive bacteria. The best antibiotic potential was found in strains of Streptomyces sp. Lim 2.2, Lim 4, Lim 5.1 and Lim 7.2, isolated from the fouling of shell rock, and strains of Streptomyces sp. Myt 7b, Myt 7ch, Myt 12a and Myt 12b isolated from mussels.

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Section: антагоністична активність чорноморських стрептоміцетів виділ...unclassified

Antagonistic Properties of the Black Sea Streptomycetes Isolated From the Fouling of Shell Rock and Mussels

Страшнова¹,

Potapenko²,

Korotaeva³

et al. 2023

mbt

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“…More than 69,000 species of fungi are described and studied, and human pathogens have found about 400 species of fungal infections, a quarter of which are the most common. To prevent this, very unpleasant diseases will only thoroughly be personal hygiene and public prevention [1][2][3][4]. The fungal cells are distinguished by the type of action between fungicidal and fungistatic agents.…”

Section: Introductionmentioning

confidence: 99%

Development of antifungal gel, composition and technology based on pomiferin metabolite isolated from fruits of Maclura aurantiaca growing in Kazakhstan

Mombekov,

Orazbekov,

Sadykova

et al. 2024

SR: PS

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The aim. The purpose of this study is to develop the optimal composition and rational gel technology under the conventional name "Kaz-P7" based on the antifungal pomiferin. Materials and methods. The object of the study is the substance pomiferin. Based on the physicochemical properties of the gel (poorly soluble in water) and technological properties structurant - Carbopol Ultrez 20 (swelling) chosen optimal solvent system: DMSO-PG-water (1:4:1) corresponding to the maximum solubility of drug and polymer provides swelling. Following the requirements of GF RK I, vol. 1, 2.2.8, 2.2.10 was determined by rotational viscometric method. Rheological properties of the sample are determined using rotational viscometer "Rheolab QC" (firm "Anton Paar", Austria) with coaxial cylinders CC27/S-SN29766 determined. The rheological parameters were studied at a temperature of 20 °C – 35 °C using the MLM U15c thermostat included in the rheostat. Results. Gel dosage forms provide better bioavailability of the drug substance. In addition, gels are a more modern dosage form, pleasant in terms of organoleptic characteristics. The technology of gel manufacturing consists of two parallel processes: preparation of the drug substance solution and its introduction into the base. From the point of view of biopharmacy, which studies the biological effect of drugs depending on their physical properties, dosage form and preparation technology, the greatest release of the drug substance occurs when it is introduced into the dosage form in dissolved form. Pomiferin administered in the dissolved state has the most therapeutic effect on the gel base. At the same time, active pharmaceutical ingredients (APIs) were dissolved in different solvents with gradual heating. The ratio of solvents DMSO-PG-water (1:4:1) at which the developed dosage form will be more structured, stable, and thixotropic gel is established. Conclusions. Fungal skin diseases are among the most frequently discussed problems in the literature. The relevance of this topic is determined by the high prevalence of pathogens, which account for 37-42 % of all skin diseases. It was experimentally established that for the complete neutralization of one gm of carbopol Ultraz 20 in the DMSO-PG-water solvent system (1:4:1), one gm of triethanolamine (pH 7.0) is consumed. The optimal diluent consisting of DMSO-PG- water in the ratio (1:4:1) was selected by physico-chemical analysis methods, and one gm of triethanolamine (pH 7.0) was selected as the neutralizing agent. The following composition was chosen as the optimal gel model: API-3.0, DMSO-3.0, PG-50.0, Carbopol Ultrez 20-1.0, and Triethanolamine-1.0. The optimal composition and technology of the gel codenamed "Kaz-P7" based on the substance obtained by pomiferin was developed

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“…Therefore, Actinobacteria living in other niches, such as the marine environment (sea sediments, coral reefs, invertebrates, etc. ), have gained value because of their chemo-diversity [ 18 , 19 , 20 , 21 ] influenced by their complex environment with extreme variations in pressure, salinity, light, and temperature [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Marine Actinobacteria a New Source of Antibacterial Metabolites to Treat Acne Vulgaris Disease—A Systematic Literature Review

2022

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Acne vulgaris is a multifactorial disease that remains under-explored; up to date it is known that the bacterium Cutibacterium acnes is involved in the disease occurrence, also associated with a microbial dysbiosis. Antibiotics have become a mainstay treatment generating the emergence of antibiotic-resistant bacteria. In addition, there are some reported side effects of alternative treatments, which indicate the need to investigate a different therapeutic approach. Natural products continue to be an excellent option, especially those extracted from actinobacteria, which represent a prominent source of metabolites with a wide range of biological activities, particularly the marine actinobacteria, which have been less studied than their terrestrial counterparts. Therefore, this systematic review aimed to identify and evaluate the potential anti-infective activity of metabolites isolated from marine actinobacteria strains against bacteria related to the development of acne vulgaris disease. It was found that there is a variety of compounds with anti-infective activity against Staphylococcus aureus and Staphylococcus epidermidis, bacteria closely related to acne vulgaris development; nevertheless, there is no report of a compound with antibacterial activity or quorum-sensing inhibition toward C. acnes, which is a surprising result. Since two of the most widely used antibiotics for the treatment of acne targeting C. acnes were obtained from actinobacteria of the genus Streptomyces, this demonstrates a great opportunity to pursue further studies in this field, considering the potential of marine actinobacteria to produce new anti-infective compounds.

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Production, Purification, and Characterization of Bioactive Metabolites Produced From Rare Actinobacteria Pseudonocardia Alni

Cited by 10 publications

References 18 publications

Antagonistic Properties of the Black Sea Streptomycetes Isolated From the Fouling of Shell Rock and Mussels

Antagonistic Properties of the Black Sea Streptomycetes Isolated From the Fouling of Shell Rock and Mussels

Development of antifungal gel, composition and technology based on pomiferin metabolite isolated from fruits of Maclura aurantiaca growing in Kazakhstan

Marine Actinobacteria a New Source of Antibacterial Metabolites to Treat Acne Vulgaris Disease—A Systematic Literature Review

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