Production, Purification and Characterization of Endopolygalacturonase by Bacillus subtillus

M, Nayab Munir Javaid Asad

doi:10.4172/2161-1009.1000181

Cited by 3 publications

(3 citation statements)

References 16 publications

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“…Glucose, fructose, galactose, and lactose were used as carbon sources to check for their effect on endo-PG production (Munir et al 2015). Endo-PG produced in the current work was purified by ammonium sulfate precipitation and size exclusion chromatography as mentioned by Hadri et al (2018) and was used to check the endo-PG activity.…”

Section: Effect Of Carbon Sources On the Endo-pg Productionmentioning

confidence: 99%

“…Hadri et al (2018) isolated B. licheniformis IEB-8 from rotten fruits and vegetables. Bacillus licheniformis IEB-8 was cultured on YEP medium (Munir et al 2015). Different growth parameters to produce endo-PG were optimized using Response Surface Methodology (RSM) under central composite design as outlined by Hadri et al (2018).…”

Section: Maintenance Of Bacillus Licheniformis Ieb-8mentioning

confidence: 99%

“…Different agriculture wastes including citrus peels, white radish peels, and apple peels were used as the only nutrient source for B. licheniformis IEB-8. The crude extracts of the three agriculture wastes were compared for endo-PG production, and the one with the highest endo-PG yield was used in subsequent experiments (Munir et al 2015).…”

Section: Use Of Agriculture Wastes For the Cheap Production Of Endo-pgmentioning

confidence: 99%

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Characterization of a novel thermophilic endopolygalacturonase produced by Bacillus licheniformis IEB-8

Hadri

Asad

Naqvi

et al. 2019

BioRes

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Endopolygalacturonases characterized until now have either low working temperatures, working pH in acidic range, high Michaelis–Menten constant (Km), or a high production cost. These characteristics are a hurdle in the industrial applications of these endopolygalacturonases. The purpose of this work was to characterize a novel endopolygalacturonase produced by Bacillus licheniformis IEB-8. Phylogenetic analysis of Bacillus licheniformis IEB-8 showed that the isolate was unique. Citrus peels were used as the only nutrient source for the growth of Bacillus licheniformis IEB-8, allowing a cheap production of endopolygalacturonase. All the synthetic carbon sources showed a negative impact on the production of endopolygalacturonase, while ammonium sulfate enhanced its production. Among different metal ions, Zn+2 showed a negative effect while Mg+2 and Ca+2 did not have any significant effect on the endopolygalacturonase activity. A Lineweaver-Burk plot was prepared for the characterization of the kinetic parameters including Km and Vmax, which were 0.45 mg/mL and 285.7 µM/min, respectively. A comprehensive comparison of the endopolygalacturonase from this study with the available literature indicated that it is better than the reported and commercially available endopolygalacturonases in having the optimum working temperature of 55 °C, a low Km of 0.57 mg/mL, and pH of 7 to 8, which indicated its novelty.

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Section: Effect Of Carbon Sources On the Endo-pg Productionmentioning

confidence: 99%

Section: Maintenance Of Bacillus Licheniformis Ieb-8mentioning

confidence: 99%

Section: Use Of Agriculture Wastes For the Cheap Production Of Endo-pgmentioning

confidence: 99%

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Characterization of a novel thermophilic endopolygalacturonase produced by Bacillus licheniformis IEB-8

Hadri

Asad

Naqvi

et al. 2019

BioRes

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Extraction of citrus pectin using pressurized carbon dioxide and production of its oligosaccharides

Pattarapisitporn,

Noma,

Klangpetch

et al. 2024

Food Bioscience

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Effect of Fermentation Response on Biosynthesis of Endopolygalacturonase from a Potent Strain of Bacillus by Utilizing Polymeric Substrates of Agricultural Origin

et al. 2022

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Endopolygalacturonase (EndoPGase), EC: 3.2.1.15. is one of the crucial pectinases belonging to the class of carbohydrase. The catalytic action of EndoPGase captivates the attention of the production of this extremely valuable catalyst in the industrial sector. The main focus was to ascertain a potential bacterial candidate for endoPGase production. The isolated bacterial strain was further identified by 16S rRNA gene sequencing. The parameters for enzyme biosynthesis were optimized by a single and multiple factor approach at a time. The results of our investigation led to the identification of a potent strain of Bacillus subtilis NR2 [strain 168]. The sequence of 16S rRNA amplified from the isolated bacterium has been submitted to GenBank under accession number ON738697. The strain was found active for pectic enzyme activity under shaking- flask fermentation at pH 5.0 and 50 °C temperature of incubation. Among all monomeric and polymeric substrates (inducer-substrates), citrus pectin, followed by potato starch and pectin (Sigma) were considered the best enzyme inducers at 1% concentration. In comparison, an increased wheat bran concentration at 5% was proved to be ideal for EndoPGase biosynthesis Moreover, an increasing trend in enzyme activity was observed with the increasing concentration of inducer. The combined effect of three variables (pH, inducer-substrates, and inducer-substrate concentration) was explored by response surface methodology (RSM) involving a Box–Behnken design (BBD). Based on the results, we concluded that the soil-isolated B. subtilis can be effectively utilized for commercial-scale pectinase enzyme biosynthesis.

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Production, Purification and Characterization of Endopolygalacturonase by Bacillus subtillus

Cited by 3 publications

References 16 publications

Characterization of a novel thermophilic endopolygalacturonase produced by Bacillus licheniformis IEB-8

Characterization of a novel thermophilic endopolygalacturonase produced by Bacillus licheniformis IEB-8

Extraction of citrus pectin using pressurized carbon dioxide and production of its oligosaccharides

Effect of Fermentation Response on Biosynthesis of Endopolygalacturonase from a Potent Strain of Bacillus by Utilizing Polymeric Substrates of Agricultural Origin

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