Production, purification, and properties of a bacteriocin from Staphylococcus aureus isolated from saliva

Abstract

Staphylococci from samples of human saliva were isolated on staphylococcalselective agar plates. These strains were tested for the inhibition of the growth of Staphylococcus aureus FDA209P. The frequency of inhibitory strains among all of the staphylococcal isolates was 5.2%. Strain IYS2, which formed the biggest inhibitory zone against the growth of the indicator strain, was used as the producer of bacteriocin. IYS2 was identified to be S. aureus, based on its biological properties. The bacteriocin was purified by sequential procedures, including ammonium sulfate precipitation, fractionation with ethanol, ion-exchange chromatography, and gel filtration. Its molecular weight was determined to be 5,000. The isoelectric point was 10.0. In amino acid composition, lysine, histidine, aspartic acid, valine, and phenylalanine were predominant. The bacteriocin was heat stable but inactivated by proteases or peptidase. The bacteriocin had a bactericidal effect on susceptible cells. An analysis of the inhibitory spectrum among typical oral indigenous bacteria showed that Streptococcus salivarius, Propionibacterium acnes, Corynebacterium parvulum, and Actinomyces israelii were susceptible to the bacteriocin. Streptococcus mutans, Streptococcus sanguis, Streptococcus mitis, Actinomyces viscosus, Actinomyces naeslundii, Fusobacterium nucleatum, Bacterionema matruchotii, and Bacteroides melaninogenicus were resistant. The majority of S. aureus tested were susceptible, and all Staphylococcus epidermidis strains tested were resistant.

Staphylococcal strains involved in bovine mastitis are inhibited by Staphylococcus aureus antimicrobial peptides

“…aureus strains, detected inhibition of only 54% of the indicator strains. A similar percentage of inhibition (52%) of staphylococcal strains was observed by Nakamura et al (1983) in relation to staphylococcin IY52. As all 65 strains involved in bovine mastitis come from distinct geographical areas and seem to © 1998 The Society for Applied Microbiology, Letters in Applied Microbiology 27, 287-291 be epidemiologically unrelated, the sensitivity of all strains to the bacteriocins encoded by A53 may suggest the presence of a particular feature in these strains which favours the antagonistic activity of these bacteriocins.…”

Genetic analysis of the bacteriocin-encoding plasmids pRJ6 and pRJ9 of Staphylococcus aureus by transposon mutagenesis and cloning of genes involved in bacteriocin production

“…A number of reports can be found on the detection, isolation, and biochemical characterization of bacteriocins produced by Staph-ylncoccus aureus ( D a W eta/. 1970;Gagliano and Hinsdill 1970;Jetten and Vogels 1973; Dajani and Taube 1974;Hale and Hinsdill 1975;Rogolski and Wiley 1977;Nakamura et al 1983;Crupper and Iandolo 1996). The first data reported in the literature correlated both bacteriocin and type B exfoliative toxin production with the presence of 42 kbp plasmids in the cells (Warren et ul.…”

Identification, characterization and purification of the lantibiotic staphylococcin T, a natural gallidermin variant

“…Within the genus Staphylococcus, bacteriocins have been reported from several species, but only a few staphylococcins have been purified and studied in detail (Lachowicz and Walczak 1968;Dajani and Wannamaker 1969;Gagliano and Hinsdill 1970;Jetten et al 1972;Hale and Hinsdill 1973;Rogolsky and Wiley 1977;Nakamura et al 1983). In recent years, there have been several reports dealing with staphylococcal bacteriocins -Pep5 (Sahl and Brandis 1981), epidermin (Allgaier et al 1986) and gallidermin ) -containing thioether amino acids such as lanthionine and methyllanthionine, and a,b-unsaturated amino acids such as didehydroalanine and didehydroaminobutyric acid.…”