Production, purification and properties of a cellulase-free thermostable endoxylanase from newly isolated Paenibacillus sp. ASCD2

Chapla, Digantkumar; Patel, Harshvadan; Singh, Atmika; Madamwar, Datta; Shah, Amita

doi:10.1007/s13213-011-0323-5

Cited by 13 publications

(11 citation statements)

References 35 publications

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“…In correlation with this study, xylanase produced by Paenibacillus sp. ASCD2 was optimally active at 60°C (Chapla et al, 2012) and also Park et al (2012) reported that 50°C was optimum for xylanase produced by Paenibacillus sp. Hpl-002.…”

Section: Resultsmentioning

confidence: 88%

Investigation on lignocellulosic saccharification and characterization of haloalkaline solvent tolerant endo-1,4 β-d-xylanase from Halomonas meridiana APCMST-KS4

Palavesam

2015

Biocatalysis and Agricultural Biotechnology

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Section: Resultsmentioning

confidence: 88%

Investigation on lignocellulosic saccharification and characterization of haloalkaline solvent tolerant endo-1,4 β-d-xylanase from Halomonas meridiana APCMST-KS4

Palavesam

2015

Biocatalysis and Agricultural Biotechnology

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“…Ninawe et al (2008) reported 1.4-fold purity of xylanase from Streptomyces cyaneus SN32 after 60% saturation with ammonium sulphate. Chapla et al (2011) also reported 13.09-fold purification of xylanase from Paenibacillus sp. ASCD2 after ammonium sulphate precipitation followed by dialysis.…”

mentioning

confidence: 91%

“…Single endoxylanase was also purified from Paenibacillus sp. ASCD2 using single-step purification by gel permeation chromatography (Chapla et al, 2011). In maximum cases, the gel filtration chromatography has been used in combination with other chromatography for purification of xylanase, however single use of gel permeation chromatography is also reported (Lee et al, 1993).…”

mentioning

confidence: 99%

Purification of Xylanases

Bajpai¹

2014

Xylanolytic Enzymes

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“…Many bacteria are reported to produce cellulase-free thermostable xylanase such as Bacillus pumilus SV-205 [7], Paenibacillus sp. ASCD2 [8], Bacillus pumilus ASH [4], Bacillus subtilis [9], Geobacillus thermoleovorans [10], and Bacillus pumilus SV-85S [11]. In this paper, we have described the isolation and identification of a potential xylanase producer: Pseudomonas boreopolis LUQ1.…”

Section: Introductionmentioning

confidence: 99%

Bacterial Xylanase in Pseudomonas boreopolis LUQ1 is Highly Induced by Xylose

Lin¹,

Shen²,

Zhu³

et al. 2017

Can J Biotech

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A xylanase producing bacterium was isolated from paper mill sludge in Thunder Bay, Canada. The newly isolated bacterium was identified as Pseudomonas boreopolis according to its 16S rRNA gene sequence and designated as LUQ1. The zymogram analysis indicated that there was one band of protein with xylanase activity, and the molecular weight of the enzyme was about 20 kDa. This xylanase works best at pH 6.0 and 65°C. The xylanase can be induced to express by xylose. The expression was enhanced on increasing the concentration of xylose, which reached its highest activity at 12 mM of xylose in fermentation. Wheat bran was the best carbon source in submerged fermentation. The highest xylanase activity of 25.61 U/ml was obtained at 96 h using wheat bran feedstock. When barley straw and wheat bran were used as feedstocks, the addition of 10 mM of xylose increased xylanase activity by ~50% and ~15%, respectively. The results showed that the strain LUQ1 has a great potential to produce xylanase for industrial applications.

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Production, purification and properties of a cellulase-free thermostable endoxylanase from newly isolated Paenibacillus sp. ASCD2

Cited by 13 publications

References 35 publications

Investigation on lignocellulosic saccharification and characterization of haloalkaline solvent tolerant endo-1,4 β-d-xylanase from Halomonas meridiana APCMST-KS4

Investigation on lignocellulosic saccharification and characterization of haloalkaline solvent tolerant endo-1,4 β-d-xylanase from Halomonas meridiana APCMST-KS4

Purification of Xylanases

Bacterial Xylanase in Pseudomonas boreopolis LUQ1 is Highly Induced by Xylose

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