Production, purification and structural study of an exopolysaccharide from Lactobacillus plantarum BC-25

Zhou, Kang; Zeng, Yiting; Yang, Mei‐Hui; Chen, Shujuan; He, Li; Ao, Xiaolin; Zou, Likou; Liu, Shuliang

doi:10.1016/j.carbpol.2016.02.067

Cited by 100 publications

(41 citation statements)

References 34 publications

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“…Harvesting the EPS at two incubation times allowed observing that the molecular mass of the EPS recovered was higher upon a longer period, but without relevant changes in monosaccharide composition, suggesting that polymerization continues even in the stationary growth phase. Analysis of previous reports about characterization of EPS produced by different strains of L. plantarum, allows concluding that most of the strains produce heteropolysaccharides when grown in media containing glucose or lactose as the carbon source (Ismail & Nampoothiri, 2010;Remus et al,2012;Tallon et al, 2003;Wang et al, 2010;Zhou et al, 2016). On the contrary, L. plantarum 70810 grown in a SD with lactose as unique carbon source produce a galactan (Wang et al, 2014).…”

Section: Discussionmentioning

confidence: 90%

Lactobacillus plantarum CIDCA 8327: An α-glucan producing-strain isolated from kefir grains

Gangoiti

Puertas

Hamet

et al. 2017

Carbohydrate Polymers

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Lactobacillus plantarum CIDCA 8327 is an exopolysaccharide (EPS)-producer strain isolated from kefir with promising properties for the development of functional foods. The aim of the present study was to characterize the structure of the EPS synthesized by this strain grown in skim milk or semidefined medium (SDM). Additionally, genes involved in EPS synthesis were detected by PCR. L. plantarum produces an EPS with a molecular weight of 10Da in both media. When grown in SDM produce an heteropolysaccharide composed mainly of glucose, glucosamine and rhamnose meanwhile the EPS produced in milk was composed exclusively of glucose indicating the influence of the sugar source. FTIR spectra of this EPS showed signals attributable to an α-glucan. Both by H NMR and methylation analysis it was possible to determine that this polysaccharide is a branched α-(1→4)-d-glucan composed of 80% linear α-(1→4)-d-glucopyranosyl units and 19% (1→4)-d-glucopyranosyl units substituted at O-3 by single α-d-glucopyranosil residues.

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Section: Discussionmentioning

confidence: 90%

Lactobacillus plantarum CIDCA 8327: An α-glucan producing-strain isolated from kefir grains

Gangoiti

Puertas

Hamet

et al. 2017

Carbohydrate Polymers

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“…EPS are the “food grade biopolymers” or high molecular weight extracellular biopolymers obtained from natural sources that are formed during the metabolic process of microorganisms, (e.g., bacteria, fungi, and blue-green algae) [ 140 , 141 ]. Among the wide variety of EPS-producing microorganisms, LAB is generally regarded as safe because of the long history of secure applicability in substances for human utilization/consumption [ 142 ].…”

Section: Quality/functional Properties Of Fermented Foodsmentioning

confidence: 99%

“…plantarum K041 isolated from traditional Chinese pickle juice originating from Kaixian possessed high yield potential for EPS production [ 142 ]. There is voluminous research, which has revealed that some EPS produced by LAB have been considered as a potential grade of bioactive natural products in the biochemical and medical applications, such as immunomodulatory, antitumor, and antioxidant effects and cholesterol lowering activities [ 140 , 145 ]. EPS from Lb.…”

Section: Quality/functional Properties Of Fermented Foodsmentioning

confidence: 99%

Lactobacillus plantarum with Functional Properties: An Approach to Increase Safety and Shelf-Life of Fermented Foods

Behera¹,

Ray²,

Zdolec

2018

BioMed Research International

314

202

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Lactobacillus plantarum (widespread member of the genus Lactobacillus) is one of the most studied species extensively used in food industry as probiotic microorganism and/or microbial starter. The exploitation of Lb. plantarum strains with their long history in food fermentation forms an emerging field and design of added-value foods. Lb. plantarum strains were also used to produce new functional (traditional/novel) foods and beverages with improved nutritional and technological features. Lb. plantarum strains were identified from many traditional foods and characterized for their systematics and molecular taxonomy, enzyme systems (α-amylase, esterase, lipase, α-glucosidase, β-glucosidase, enolase, phosphoketolase, lactase dehydrogenase, etc.), and bioactive compounds (bacteriocin, dipeptides, and other preservative compounds). This review emphasizes that the Lb. plantarum strains with their probiotic properties can have great effects against harmful microflora (foodborne pathogens) to increase safety and shelf-life of fermented foods.

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“…plantarum YW32 were mannose, fructose, galactose, and glucose at a ratio of 8.2 : 1 : 4.1 : 4.2. 40) According to another research group, the EPS produced by Lb. plantarum BC-25 was composed of glucose, galactose, and mannose at a molar ratio of 6.0 : 1.79 : 92.21.…”

Section: Eps-biosynthesizing Gene Cluster Detected In the Lb Plantarmentioning

confidence: 99%

Characterization of the SN35N Strain-Specific Exopolysaccharide Encoded in the Whole Circular Genome of a Plant-Derived <i>Lactobacillus plantarum</i>

Noda

Shiraga

Kumagai

et al. 2018

Biological & Pharmaceutical Bulletin

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Lactobacillus plantarum SN35N, which has been previously isolated from pear, secretes exopolysaccharide (EPS). The aim of the present study is to characterize the EPS chemically and to find the EPS-biosynthesizing gene cluster. The present study demonstrates that the strain produces an acidic EPS carrying phosphate residue, which is composed of glucose, galactose, and mannose at a molecular ratio of 15.0 : 5.7 : 1.0. We also show that acidic EPS strongly inhibits the catalytic activity of hyaluronidase (EC 3.2.1.35), promoting an inflammatory reaction. In the present study, we also determined the complete genome sequence of the SN35N strain, demonstrating that the genome is a circular DNA with 3267626 bp, and the number of predicted coding genes is 3146, with a GC content of 44.51%. In addition, the strain harbors four plasmids, designated pSN35N-1, -2, -3, and -4. Although four EPS-biosynthesizing genes, designated lpe1, lpe2, lpe3, and lpe4, are present in the SN35N chromosomal DNA, another EPS gene cluster, lpe5, is located in the pSN35N-3 plasmid, composed of 35425 bp. EPS low-producing mutants, which were obtained by treating SN35N cells with novobiocin, lost the lpe5 gene cluster in the plasmid-curing experiment, suggesting that the gene cluster for the biosynthesis of acidic EPS is present in the plasmid. The present study shows the chemical characterization of the acidic EPS and its inhibitory effect to the hyaluronidase.

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Production, purification and structural study of an exopolysaccharide from Lactobacillus plantarum BC-25

Cited by 100 publications

References 34 publications

Lactobacillus plantarum CIDCA 8327: An α-glucan producing-strain isolated from kefir grains

Lactobacillus plantarum CIDCA 8327: An α-glucan producing-strain isolated from kefir grains

Lactobacillus plantarum with Functional Properties: An Approach to Increase Safety and Shelf-Life of Fermented Foods

Characterization of the SN35N Strain-Specific Exopolysaccharide Encoded in the Whole Circular Genome of a Plant-Derived <i>Lactobacillus plantarum</i>

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