Production Quality Control of Mixed Culture Poly(3-Hydroxbutyrate-co-3-Hydroxyvalerate) Blends Using Full-Scale Municipal Activated Sludge and Non-Chlorinated Solvent Extraction

Werker, Alan; Bengtsson, Stina; Johansson, Peter; Magnusson, Per; Gustafsson, Emma; Hjort, Markus; Anterrieu, Simon; Karabegovic, Lamija; Alexandersson, Tomas; Karlsson, Anton; Morgan-Sagastume, Fernando; Sijstermans, Luc; Tietema, Martin; Wypkema, Etteke; Kooij, Yede van der; Deeke, Alexandra; Uijterlinde, C. A.; Korving, Leon

doi:10.1201/9780429296635-13

Cited by 5 publications

(9 citation statements)

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“…The pulse-wise feeding rate was coupled to the substrate uptake rate based on dissolved oxygen concentration as a proxy for the biomass oxygen uptake rate . The relative area of floc PHA granule coverage with respect to the nonstained biomass increased markedly over the course of the accumulation process.…”

Section: Resultsmentioning

confidence: 99%

“…The PHA content in a dried biomass sample is readily quantified. Biomass PHA content is frequently reported as a part of accumulation performance assessments. , Enrichment accumulation methods at the laboratory scale have achieved a biomass PHA content of 90% gPHA/gVSS . However, biomass PHA content under industrial conditions is often lower and can range between 40 and 80% gPHA/gVSS. ,, In these enrichment cultures, most of the biomass typically stores PHA.…”

Section: Introductionmentioning

confidence: 99%

“…They are stored as intracellular granules and provide intermediate reservoirs for the supply of carbon and energy. − Microorganisms that accumulate PHA do so typically when environmental conditions result in stresses, such as a lack of an essential nutrient, while organic carbon is available in excess. − PHAs recovered from PHA-rich biomass exhibit thermoplastic and mechanical properties similar to petroleum-based polyesters commonly used today. , Distinct from petroleum-derived polymers, PHAs are bio-based and biodegradable. Research and development efforts advance to realize the potential for PHA production using wastewater as feedstock and microbial community-based production methods. , …”

Section: Introductionmentioning

confidence: 99%

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Visualization of Polyhydroxyalkanoate Accumulated in Waste Activated Sludge

Pei

Vicente-Venegas²,

Tomaszewska-Porada³

et al. 2023

Environ. Sci. Technol.

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Polyhydroxyalkanoates (PHAs) can be produced with municipal waste activated sludge from biological wastewater treatment processes. Methods of selective fluorescent staining with confocal laser scanning microscopy (CLSM) were developed and optimized to evaluate the distribution of PHA storage activity in this mixed culture activated sludge microbial communities. Selective staining methods were applied to a municipal activated sludge during pilot scale PHA accumulation in replicate experiments. Visualization of stained flocs revealed that a significant but limited fraction of the biomass was engaged with PHA accumulation. Accumulated PHA granules were furthermore heterogeneously distributed within and between flocs. These observations suggested that the PHA content for the bacteria storing PHAs was significantly higher than the average PHA content measured for the biomass as a whole. Optimized staining methods provided high acuity for imaging of PHA distribution when compared to other methods reported in the literature. Selective staining methods were sufficient to resolve and distinguish between distinctly different morphotypes in the biomass, and these observations of distinctions have interpreted implications for PHA recovery methods. Visualization tools facilitate meaningful insights for advancements of activated sludge processes where systematic observations, as applied in the present work, can reveal underlying details of structure–function relationships.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Visualization of Polyhydroxyalkanoate Accumulated in Waste Activated Sludge

Pei

Vicente-Venegas²,

Tomaszewska-Porada³

et al. 2023

Environ. Sci. Technol.

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“…The VFA composition strongly influences the accumulated co-polymer monomer distribution and, thereby, resultant co-polymer blend crystallinity. Even full-scale waste municipal activated sludge has been shown to be directly applicable to produce commercial quality PHAs [17]. Evidence was given for engineering polymer properties and maintaining quality control with VFA feedstocks produced from fermented sludge and industrial waste at pilot scale.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, so long as the dilute VFA rich feedstock supplies do not require undue transportation, it is only necessary that a sufficient VFA mass is available, even if it is in a dilute form. A sufficient VFA mass is to be delivered in a volumetric flow at industrial scale to exploit the PHA storing potential of a MMC biomass such as surplus municipal activated sludge [17]. Activated sludge thickened (concentrated) biomass solids, with at least 5 to 10% dry solids content, may be brought within reasonable distance to the site of PHA production where available VFA sources exist.…”

Section: Introductionmentioning

confidence: 99%

Modelling Mixed Microbial Culture Polyhydroxyalkanoate Accumulation Bioprocess towards Novel Methods for Polymer Production Using Dilute Volatile Fatty Acid Rich Feedstocks

et al. 2022

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Volatile fatty acid (VFA) rich streams from fermentation of organic residuals and wastewater are suitable feedstocks for mixed microbial culture (MMC) Polyhydroxyalkanoate (PHA) production. However, many such streams have low total VFA concentration (1–10 gCOD/L). PHA accumulation requires a flow-through bioprocess if the VFAs are not concentrated. A flow through bioprocess must balance goals of productivity (highest possible influent flow rates) with goals of substrate utilization efficiency (lowest possible effluent VFA concentration). Towards these goals, dynamics of upshift and downshift respiration kinetics for laboratory and pilot scale MMCs were evaluated. Monod kinetics described a hysteresis between the upshift and downshift responses. Substrate concentrations necessary to stimulate a given substrate uptake rate were significantly higher than the concentrations necessary to sustain the attained substrate uptake rate. A benefit of this hysteresis was explored in Monte Carlo based PHA accumulation bioprocess numerical simulations. Simulations illustrated for a potential to establish continuous flow-through PHA production bioprocesses even at a low (1 gCOD/L) influent total VFA concentration. Process biomass recirculation into an engineered higher substrate concentration mixing zone, due to the constant influent substrate flow, enabled to drive the process to maximal possible PHA production rates without sacrificing substrate utilization efficiency.

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