Productive performance of the dairy cattle Girolando breed mediated by the fat-related genes DGAT1 and LEP and their polymorphisms

Cardoso, Sergio; Queiroz, Luana Benedetti de; Alonso-Goulart, Vivian; Mourão, Gerson Barreto; Benedetti, Edmundo; Goulart, Luíz Ricardo

doi:10.1016/j.rvsc.2011.02.006

Cited by 7 publications

(7 citation statements)

References 31 publications

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“…When the least square mean of the traits was considered (Table 2), genotype KK gives the most effect on milk composition content traits, while genotype AA has the most effect on milk yield and milk composition yield traits. This result was in accordance with the studies of Cardoso et al (2011), Streit et al (2011, Barbosa et al (2010), Banos et al (2008), Bennewitz et al (2004), Gautier et al (2007), Grisart et al (2002), Kuehn et al (2007), Spelman et al (2002), Thaller et al (2003), and Weller et al (2003) that found that this locus, particularly the KK genotype, has the effect of increasing fat content. These results suggest that the effect of the DGAT1 gene does not only affect milk fat content but also affects the milk composition, particularly, milk protein.…”

Section: Resultssupporting

confidence: 92%

“…These results contrast with the study of Schennink et al (2007) and Sun et al (2009). While the studies of Cardoso et al (2011) and Streit et al (2011) found that the K allele of the DGAT1 gene showed a significant association with milk production. Although the results from many studies are inconclusive, it is clear that the DGAT1 gene has a strong association with milk production and milk composition traits.…”

Section: Introductioncontrasting

confidence: 88%

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Effects of Acyl-CoA:diacylglycerol acyl transferase 1 (DGAT1) gene on milk production traits in crossbred Holstein dairy cattle

Molee

Duanghaklang

Na-Lampang

2011

Trop Anim Health Prod

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The objective of this study was to analyze and investigate the genotype frequency and the association between Acyl-CoA:diacylglycerol acyltransferase1 gene, DGAT1 gene, and milk yield (MY), milk composition, protein yield (PY), fat yield (FY), solid not fat yield (SNF), total solid (TS), the content of fat, protein, solid not fat, and total solid, (%Fat,%Prot,%SNF,%TS) in two herds of crossbred Holstein dairy cattle in Thailand. Two hundred and twenty-seven crossbred Holstein cows were used and their blood samples were taken for the study. PCR-RFLP was used to identify the allele and genotype of DGAT1 gene. A general linear model and the least square method were used to estimate the least square mean and additive, and the dominant effect of the gene on the traits and the least significant differences were used to compare the mean of each trait between genotypes. Two alleles (K, A) and three genotypes (AA, KA, KK) were detected, the highest allele and genotype frequencies were A and AA, respectively. The least mean squares of each genotype were compared and significant differences between genotype were detected. Genotype KK has the greatest effect on all milk composition content traits, while genotype AA has the greatest effect on yield traits. Highly significant additive gene effect was detected. From the results, it can be concluded that the DGAT1 gene can be used as a gene marker for assisted selection in milk composition traits.

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Section: Resultssupporting

confidence: 92%

Section: Introductioncontrasting

confidence: 88%

Effects of Acyl-CoA:diacylglycerol acyl transferase 1 (DGAT1) gene on milk production traits in crossbred Holstein dairy cattle

Molee

Duanghaklang

Na-Lampang

2011

Trop Anim Health Prod

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“…Another well described SNP affecting FA profile at 50% concentration [6], is K232A mutation in DGAT1 gene, which is a nonsynonymous non-conservative substitution of lysine to alanine [20]. Bouwman et al [11] observed that between 63rd and 282nd DIM, allele K was connected with a higher percentage of C6:0, C8:0, C16:0, and C16:1 fractions, as well as with a lower percentage of C14:0, C18:1, and CLA fractions.…”

Section: Dgat1 Gene Effectmentioning

confidence: 99%

Impact of SNPs in ACACA, SCD1, and DGAT1 Genes on Fatty Acid Profile in Bovine Milk with Regard to Lactation Phases

Kęsek-Woźniak

Wojtas

Zielak-Steciwko

2020

Animals

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Milk fat is a dietary source of fatty acids (FA), which can be health promoting or can increase risks of some diseases. FA profile composition depends on many factors, among them gene polymorphism. This study analyzed the relation between polymorphism of acetyl-CoA carboxylase α (ACACA), stearoyl-CoA desaturase 1 (SCD1), diacylglycerol acyltransferase 1 (DGAT1) genes with FA profile in milk from Polish Holstein-Friesian cattle and determined changes of FA percentage during lactation with regard to polymorphism. Milk samples were collected twice: during the first phase of lactation (<90 Days in milk; DIM) and at the end of lactation (>210 DIM). During the first milk collection, blood samples were taken to analyze three chosen single nucleotide polymorphisms (SNPs): AJ312201.1g.1488C > G SNP in ACACA gene, A293V SNP in SCD1 gene, and K232A SNP in DGAT1 gene. Increased concentration of FA that are less beneficial for human health and have lower concentration of healthy FA in homozygotes: GG in ACACA, VV in SCD1, and KK in DGAT1 were observed, as well as a strong influence of the analyzed genes on FA with 18C atoms was also found. Moreover, it was demonstrated that lactation phase significantly affected FA percentage in milk depending on the phenotype. These results may contribute their part to knowledge toward obtaining more beneficial milk composition.

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“…The DGAT1 K polymorphism was found to associate with a higher fat percentage, higher fat yield, higher protein percentage, lower protein yield, and lower milk yield (Grisart et al, 2002;Schennink et al, 2007). Many studies have investigated the effect of DGAT1 polymorphism on milk production, with a focus on fat production (e.g., in different cow breeds; Berry et al, 2010;Cardoso et al, 2011;Komisarek et al, 2011;Molee et al, 2012). In addition, a recent paper by Argov-Argaman et al (2013) described the effect of the DGAT1 genotype and fat content on the composition of the milk fat globule membrane (MFGM).…”

Section: Introductionmentioning

confidence: 99%

Effect of the DGAT1 K232A genotype of dairy cows on the milk metabolome and proteome

Lü

Boeren

Hooijdonk

et al. 2015

Journal of Dairy Science

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Diglyceride O-acyltransferase 1 (DGAT1) is the enzyme that catalyzes the synthesis of triglycerides from diglycerides and acyl-coenzyme A. The DGAT1 K232A polymorphism was previously shown to have a significant influence on bovine milk production characteristics (milk yield, protein content, fat content, and fatty acid composition). The mechanism of this influence has, however, not been elucidated. In this study, metabolomics ((1)H-nuclear magnetic resonance) and proteomics (laser chromatography-tandem mass spectrometry) were applied to determine the serum and lipid metabolite composition and milk fat globule membrane proteome of milk samples from cows with the DGAT1 KK and AA genotypes. The milk samples from cows with the DGAT1 KK genotype contained more stomatin, sphingomyelin, choline, and carnitine, and less citrate, creatine or phosphocreatine, glycerol-phosphocholine, mannose-like sugar, acetyl sugar phosphate, uridine diphosphate (UDP)-related sugar, and orotic acid compared with milk samples from cows with the DGAT1 AA genotype. Based on these results, we propose that the differences between the DGAT1 genotypes may be related to stomatin-sphingomyelin lipid rafts as well as structural (cell membrane) differences in epithelial cells of the mammary gland. In conclusion, our study shows that, in addition to previously described changes in triglyceride composition, cows differing in DGAT1 polymorphism differ in their milk proteome and metabolome, which may help in further understanding the effect of the DGAT1 K232A polymorphism on milk production characteristics.

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Productive performance of the dairy cattle Girolando breed mediated by the fat-related genes DGAT1 and LEP and their polymorphisms

Cited by 7 publications

References 31 publications

Effects of Acyl-CoA:diacylglycerol acyl transferase 1 (DGAT1) gene on milk production traits in crossbred Holstein dairy cattle

Effects of Acyl-CoA:diacylglycerol acyl transferase 1 (DGAT1) gene on milk production traits in crossbred Holstein dairy cattle

Impact of SNPs in ACACA, SCD1, and DGAT1 Genes on Fatty Acid Profile in Bovine Milk with Regard to Lactation Phases

Effect of the DGAT1 K232A genotype of dairy cows on the milk metabolome and proteome

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