The aim of the study was to evaluate soybean performance and canonical interrelationships towards spacing between sowing lines. This study was developed in the 2019/2020 crop season, in the county of Lagoa da Confusão, Tocantins State, Brazil. The experimental design used was randomized blocks with four replications, using five spacing between lines (45, 35, 25 21, and 17 centimeters), conserving the same plant population. The plots consisted of four lines ten meters long. There was 30 cm between rows, demonstrating that it is attractive for soybean sowing. It should be noted that the reduction in the spacing of the sowing line leads to an increase in costs and operating time, which can make this recommendation unfeasible. With the objective of carrying out two consecutive plantings in the rainy season, it causes the rural producer to neglect some agronomic practices that increase productivity, especially when they result from increased operational time.