Triticale is a fodder cereal with a high nutritional value of grains. It can also be used for food purposes, so the chemical composition of the grain is important. The aim of the study was to determine the changes in the macroelement content in the grains of winter triticale cultivars under the use of different tillage methods and the percentage of cereals in the crop rotation. The research shows that the chemical composition of grains depends on the weather conditions, tillage method, and the share of cereals in crop rotation, as well as the genetic factor. In the year of the study, with the highest precipitation in the growing period, the highest concentrations of magnesium and phosphorus were found. The accumulation of nitrogen and sodium in triticale grain was higher in the year with lower precipitation. In crop rotation with a lower percentage of cereals, higher concentrations of nitrogen, potassium, phosphorus, and calcium were found. The concentrations of nitrogen, phosphorus and magnesium did not depend on the method of soil tillage, whereas in ploughing conditions, higher concentrations of potassium, calcium and sodium were found. Alekto cultivar was characterized by a higher content of macroelements (nitrogen and sodium) in the grains, while Fredro cultivar, of calcium. The content of phosphorus in grain was similar in Alekto and Cerber cultivars.