Products from Basidiomycetes

Erkel, Gerhard; Anke, Timm

doi:10.1002/9783527620999.ch11g

Cited by 3 publications

(3 citation statements)

References 143 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As indicated in the present study, using standard methods based on cultural, morpho-microscopic, ITS nrDNA gene sequencing and phylogenetic analysis, we have successfully isolated, and characterized P. caribaeo-quercicola as an endophyte from the leaves of I. racemosa. Fungi belonging to the Basidiomycota phylum typically exhibit poor growth under laboratory conditions [34]. Macroscopically the surface texture and colour of P. caribaeo-quercicola INL3-2 strain isolated in the current study were found to be the same as that described for P. caribaeo-quercicola by others [35].…”

Section: Antioxidant Activitysupporting

confidence: 81%

Evaluating the Biocontrol Efficacy and Antioxidant Potential of Phellinus caribaeo‐quercicola—A First Report Dual‐Action Endophyte From Inula racemosa Hook. F.

Majid,

Wani,

Ganai

2024

J Basic Microbiol

View full text Add to dashboard Cite

Phellinus caribaeo‐quercicola is a basidiomycetous fungus, isolated as an endophyte in this study from the healthy and symptomless leaves of Inula racemosa Hook. f., an important medicinal herb growing in Kashmir Himalaya. This study combines morphological, molecular and phylogenetic techniques to identify the fungal endophyte, using the ITS sequence of nrDNA. A detached leaf assay was conducted to assess the pathogenicity of the fungal endophyte suggesting its mutually symbiotic relationship with the host. The authors also investigated the antifungal potential of the isolated endophytic strain to ascertain its use as a biocontrol agent. The study shows that P. caribaeo‐quercicola INL3‐2 strain exhibits biocontrol activity against four key fungal phytopathogens that cause significant agronomic and economic losses: Aspergillus flavus, Aspergillus niger, Fusarium solani, and Fusarium oxysporum. Notably, P. caribaeo‐quercicola INL3‐2 strain is highly effective against A. flavus, with an inhibition percentage of 57.63%. In addition, this study investigates the antioxidant activity of P. caribaeo‐quercicola INL3‐2 strain crude extracts using ethyl acetate and methanol as solvents. The results showed that the methanolic fraction of P. caribaeo‐quercicola exhibits potential as an antioxidant agent, with an IC50 value of 171.90 ± 1.15 µg/mL. This investigation is first of its kind and marks the initial report of this fungal basidiomycete, P. caribaeo‐quercicola, as an endophyte associated with a medicinal plant. The findings of this study highlight the potential of P. caribaeo‐quercicola INL3‐2 strain as a dual‐action agent with both biocontrol and antioxidant properties consistent with the medicinal properties of Inula racemosa. This endophytic fungus could be a promising source of natural compounds for use in agriculture, medicine, and beyond.

show abstract

Section: Antioxidant Activitysupporting

confidence: 81%

Evaluating the Biocontrol Efficacy and Antioxidant Potential of Phellinus caribaeo‐quercicola—A First Report Dual‐Action Endophyte From Inula racemosa Hook. F.

Majid,

Wani,

Ganai

2024

J Basic Microbiol

View full text Add to dashboard Cite

show abstract

“…lineata strains. Interestingly, this clade also includes some uncultured fungi which is not unexpected since a considerable number of fungi belonging to the Basidiomycota phylum frequently show poor growth under laboratory conditions [40]. According to the features described by Reck and Rosa [41], the basidiospores of Grammothele lineata are 2.5–3 μm thick matching with the basidiospores of our strain, SDL-CO-2015-1.…”

Section: Discussionsupporting

confidence: 60%

An endophytic Basidiomycete, Grammothele lineata, isolated from Corchorus olitorius, produces paclitaxel that shows cytotoxicity

et al. 2017

View full text Add to dashboard Cite

Grammothele lineata, an endophyte isolated in our laboratory from jute (Corchorus olitorius acc. 2015) was found to be a substantial paclitaxel producer. Taxol and its related compounds, produced by this endophyte were extracted by growing the fungus in simple nutrient media (potato dextrose broth, PDB). Taxol was identified and characterized by different analytical techniques (TLC, HPLC, FTIR, LC-ESI-MS/MS) following its extraction by ethyl acetate. In PDB media, this fungus was found to produce 382.2 μgL-1 of taxol which is about 7.6 x103 fold higher than the first reported endophytic fungi, Taxomyces andreanae. The extracted taxol exhibited cytotoxic activity in an in vitro culture of HeLa cancer cell line. The fungal extract also exhibited antifungal and antibacterial activities against different pathogenic strains. This is the first report of a jute endophytic fungus harboring the capacity to produce taxol and also the first reported taxol producing species that belongs to the Basidiomycota phylum, so far unknown to be a taxol producer. These findings suggest that the fungal endophyte, Grammothele lineata can be an excellent source of taxol and can also serve as a potential species for chemical and genetic engineering to enhance further the production of taxol.

show abstract

“…Compound 7 can be oxidized and acetylated to the antibacterial sesquiterpenoid naematolon (Figure ). Compound 9 is the precursor of (+)-costunolide, which has been investigated for a wide range of biological activities that are owing to its modulation of various intracellular signaling pathways by targeting intracellular kinases and redox-regulated transcription factors . α-Bisabolol derived from compound 15 has a strong time- and dose-dependent cytotoxic effect on human and rat glioma cells .…”

Section: Discussionmentioning

confidence: 99%

Structural Insights into Three Sesquiterpene Synthases for the Biosynthesis of Tricyclic Sesquiterpenes and Chemical Space Expansion by Structure-Based Mutagenesis

Lou

et al. 2023

J. Am. Chem. Soc.

View full text Add to dashboard Cite

The cyclization of farnesyl diphosphate (FPP) into highly strained polycyclic sesquiterpenes is challenging. We here determined the crystal structures of three sesquiterpene synthases (STSs, namely, BcBOT2, DbPROS, and CLM1) catalyzing the biosynthesis of the tricyclic sesquiterpenes presilphiperfolan-8β-ol (1), Δ6-protoilludene (2), and longiborneol (3). All three STS structures contain a substrate mimic, the benzyltriethylammonium cation (BTAC), in their active sites, providing ideal templates for quantum mechanics/molecular mechanics (QM/MM) analyses toward their catalytic mechanisms. The QM/MM-based molecular dynamics (MD) simulations revealed the cascade reactions toward the enzyme products, and different key active site residues that play important roles in stabilizing reactive carbocation intermediates along the three pathways. Site-directed mutagenesis experiments confirmed the roles of these key residues and concomitantly resulted in 17 shunt products (4–20). Isotopic labeling experiments addressed the key hydride and methyl migrations toward the main and several shunt products. These combined methods provided deep insights into the catalytic mechanisms of the three STSs and demonstrated how the chemical space of STSs can rationally be expanded, which may facilitate applications in synthetic biology approaches toward pharmaceutical and perfumery agents.

show abstract

Products from Basidiomycetes

Cited by 3 publications

References 143 publications

Evaluating the Biocontrol Efficacy and Antioxidant Potential of Phellinus caribaeo‐quercicola—A First Report Dual‐Action Endophyte From Inula racemosa Hook. F.

Evaluating the Biocontrol Efficacy and Antioxidant Potential of Phellinus caribaeo‐quercicola—A First Report Dual‐Action Endophyte From Inula racemosa Hook. F.

An endophytic Basidiomycete, Grammothele lineata, isolated from Corchorus olitorius, produces paclitaxel that shows cytotoxicity

Structural Insights into Three Sesquiterpene Synthases for the Biosynthesis of Tricyclic Sesquiterpenes and Chemical Space Expansion by Structure-Based Mutagenesis

Contact Info

Product

Resources

About