We provide a unified and exact framework for the variance-based uncertainty relations. This unified framework not only recovers some well-known previous uncertainty relations, but also fixes the deficiencies of them. Utilizing the unified framework, we can construct the new uncertainty relations in both product and sum form for two and more incompatible observables with any tightness we require. Moreover, one can even construct uncertainty equalities to exactly express the uncertainty relation by the unified framework, and the framework is therefore exact in describing the uncertainty relation. Some applications have been provided to illustrate the importance of this unified and exact framework. Also, we show that the contradiction between uncertainty relation and non-Hermitian operator, i.e., most of uncertainty relations will be violated when applied to non-Hermitian operators, can be fixed by this unified and exact framework. Quantum uncertainty relations 1-3 , expressing the impossibility of the joint sharp preparation of the incompatible observables 4,5 , are the most fundamental differences between quantum and classical mechanics 6-9. The uncertainty relation has been widely used in the quantum information science 10,11 , such as quantum non-cloning theorem 12,13 , quantum cryptography 14-17 , entanglement detection 18-22 , quantum spins squeezing 23-26 , quantum metrology 27-29 , quantum synchronization 30,31 and mixedness detection 32,33. In general, the improvement in uncertainty relations will greatly promote the development of quantum information science 18,28,34-36. The variance-based uncertainty relations for two incompatible observables A and B can be divided into two forms: the product form ΔA 2 ΔB 2 ≥ LB p 2,3,5,37,38 and the sum form ΔA 2 + ΔB 2 ≥ LB s 39-42 , where LB p and LB s represent the lower bounds of the two forms uncertainty relations, and ΔQ 2 is the variance of Q (To make sure that the quantity measuring the uncertainty will be a real number, the variance is taken as 〈(Q − 〈Q〉) † (Q − 〈Q〉) for non-Hermitian operators. Here the 〈Q〉 represents the expected value of Q). The product form uncertainty relation cannot fully capture the concept of the incompatible observables, because it can be trivial; i.e., the lower bound LB p can be null even for incompatible observables 39,40,43,44. This deficiency is referred to as the triviality problem of the product form uncertainty relation. In order to fix the triviality problem, Maccone and Pati deduced a sum form uncertainty relation with a non-zero lower bound for incompatible observables 44 , firstly showing that the triviality problem can be addressed by the sum form uncertainty relation. Thus, the sum form uncertainty relations were considered to be stronger than the product form uncertainty relations, and since then, lots of effort has been made to investigate the uncertainty relation in the sum form 18,39,45-48. However, most of the sum form uncertainty relations depend on the orthogonal state to the state of the system, and thus are diffic...