Produksi Enzim Fibrinolitik Tempe oleh Rhizopus oryzae FNCC 6078

Abstract

ackground Rhizopus oryzae FNCC 6078 had been evaluated producing fibrinolytic enzyme under solid state fermentation. Soybean had been used to produce fibrinolytic enzyme through fermentation in tempeh. The main purpose of this study was to reveal optimum condition for fermentation. The parameters of the condition were inoculum volume, incubation period and temperature. Optimum condition was defined by maximum fibrinolytic activity. Methode Fibrinolytic activity was measured using spectrophotometry at 274 nm. Result optimum condition for producing fibrinolytic enzyme was 1,5 mL volume of inoculum of Rhizopus oryzae suspension in 25%T, 42 hours for incubation period and 35oC temperature incubation.

Fibrinolytic Protease Production: Impact of Initial pH and Temperature in Solid-State Fermentation by Rhizopus microsporus var. oligosporus FNCC 6010

“…0.000 (< 0.05). Similar research results revealed that Rhizopus oryzae FNCC 6078 for fibrinolytic enzyme production increased with increasing incubation temperature, and the optimum temperature was reached at 35 °C (Sada et al, 2021). Environmental temperature is one of the factors that greatly affect the growth and metabolism of organisms (Srivastava et al, 2019).…”

“…The fibrin media was pasteurized for 3 minutes at 80±2 °C. About 20 mL of fibrin media was poured into a sterile petri dish method modified (Poernomo et al, 2021). Fibrinolytic activity assay was done by pipetting a 40µl sample or control to each hole of fibrin media on a petri dish.…”

“…oligosporus made fibrinolytic enzymes by fermentation (Sarao et al, 2010). Other studies have revealed that Rhizopus species can produce fibrinolytic proteases, including R. oryzae (Sada et al, 2021); Rhizopus chinensis 12 (Xiao-Lan et al, 2005); R. microsporus var. tuberosus (Zhang et al, 2015); and Rhizopus oligosporus (Poernomo et al, 2017).…”

“…During the fermentation process, the incubation temperature has an impact on the metabolic activity of microorganisms and the growth of fungi (Rauf et al, 2010). Temperature also affects the stability of the enzymes produced (Kotb, 2013;Sada et al, 2021). Furthermore, an increase of incubation temperature causes a rise of enzyme activity up to a certain point, and after that, there can be an effect on the growth of microorganisms and allow enzyme denaturation to occur (Vinuthna & Raju, 2022).…”

Fibrinolytic Protease Activity of Crude Enzyme from Fermented Sunflower (Helianthus annuus) and Common Bean (Phaseolus vulgaris) seeds by Rhizopus microsporus var. oligosporus FNCC 6010 in Solid State Fermentation

“…Rhizopus sp. has been reported to produce fibrinolytic proteases, including R. oryzae 9 ; R. chinensis Fermentation is the process of breaking down large organic molecules with the help of microorganisms into simpler molecules 18 that are used to produce microbial metabolites 5 . The SSF process is carried out on a solid substrate with certain environmental conditions 19 .…”