A total of 37 different poplar varieties were grown in a randomized mini-rotation short rotation coppice (SRC) (harvest every three years) on a light sandy soil under continental climatic conditions in the south of the Federal State of Brandenburg, Germany. Along with well-known poplar varieties, newly bred ones that have not yet been approved for commercial use were selected for this study. Survival rates were determined after the first growing season in 2013 as well as at the first and second harvests in 2015 and 2018. Furthermore, the number of shoots, plant height, diameter at breast height, dry matter content and biomass yield of the varieties were recorded. After the second rotation period, only seven poplar varieties yielded more than 11 tadm ha−1 y−1 and can be recommended for commercial use. However, many varieties only reached about 8 tadm ha−1 y−1, and six varieties even had less than 4 tadm ha−1 y−1, among them newly bred varieties. Given the changing climate conditions, the cultivation of these varieties in SRC is not recommended. Our data also show that the biomass yield of several varieties decreased from the first to the second harvests. Since the survival rates were high and no damage by pest species was observed, the site-specific yield capacities of the individual clones are assumed to be the cause for this.