Lack of fertile soil is among the main problems in world agricultural production areas, mainly in vegetable crop production areas. In this sense, we aimed to evaluate the the production of chili (Capsicum annuum L.) using cow urine as an organic fertilizer. The survey was conducted from June to September 2014 at the School State of Primary and Secondary Nossa Senhora da Conceição, Belém de Brejo do Cruz-PB, Brazil. The experimental design was completely randomized (DIC), in 5 x 2 factorial scheme of 40 plants, with seven repetitions. There was a total of 10 treatments. The effects of 5 cow urine doses were studied: 0, 25, 50, 75 and 100 ml, applied to soil combined with fertilizers: A1 = wood powder + cattle manure + sand washed (1:1:1) and A2 = cattle manure + sand washed (2:1)). There was a significant effect at 1% probability in F test for cow urine doses in all variables. Except the fruit length and number of unmarketable fruits, organic fertilizers statistically influenced the level of p <0.01 in all variables. There was no significant response to the interaction doses × organic fertilizers. 100 ml of cow urine provided good results in the production of chili. The organic manure fertilizer bovine + washed sand is great for chili culture. it is not recommended to incorporate wood powder organic fertilizers.