PROFIL METABOLIT BERBAGAI EKSTRAK DAUN Chrysophyllum cainito L. MENGGUNAKAN UPLC-QTOF-MS/MS

Abstract

<jats:p>ABSTRACT&#x0D; Chrysophyllum cainito L. is a plant which empirically used as traditional medicine. The pharmacological effect of C. cainito is caused by secondary metabolite activity contain in the leaves. The aim of this research was to know the metabolites profile in n-hexane extract, ethyl acetate extract, and methanol extract of C. cainito leaves using UPLC-QToF-MS/MS. Dried powder of C. cainito leaves was extracted with n-hexane, ethyl acetate, and methanol with gradual extraction using Ultrasonic Assisted Extraction (UAE). Each extract was prepared with methanol and DCM solvent then injected 5 µl into UPLC-QToF-MS/MS and analyzed with Masslynx 4.1 softwares and Chemspider. The result showed that there were 28 compounds from n-hexane extract with diethyltoluamide as major compound, 47 compounds from ethyl acetate extract with loliolide as major compound, and 34 compounds from methanol extract with eplerenone as major compound. Based on literature study, there were also several compounds that likely having activity as phytoestrogens.&#x0D; Keyword: Metabolite profiling, chrysophyllum cainito L., UPLC QToF-MS/MS&#x0D; &#x0D; &#x0D; ABSTRAK&#x0D; Chrysophyllum cainito L. merupakan tumbuhan yang secara empiris digunakan sebagai obat tradisional. Efek farmakologi tersebut disebabkan adanya aktivitas dari berbagai senyawa metabolit sekunder yang terkandung dalam daun C. cainito. Tujuan dari penelitian ini untuk mengetahui profil metabolit ekstrak n-heksana, ekstrak etil asetat, dan ekstrak metanol daun C. cainito menggunakan UPLC-QToF-MS/MS. Serbuk kering daun C. cainito diekstraksi secara bertingkat menggunakan n-heksana, etil asetat, dan metanol dengan metode UAE. Masing-masing ekstrak dipreparasi dengan metanol dan DCM lalu diinjeksikan sebanyak 5 µl ke dalam UPLC-QToF-MS/MS, kemudian dianalisis dengan software Masslynx 4.1 dan Chemspider. Hasil menunjukkan profil metabolit dari masing-masing ekstrak daun C. cainito, yaitu ekstrak n-heksana dengan 28 senyawa dan diethyltoluamide sebagai senyawa mayor, 47 senyawa terkandung dalam ekstrak etil asetat dengan senyawa mayor loliolide, dan 34 senyawa terkandung dalam ekstrak metanol dengan senyawa mayor yaitu eplerenone. Dari studi literatur diketahui terdapat beberapa senyawa yang memiliki aktivitas sebagai fitoestrogen.&#x0D; Kata Kunci: Metabolite profiling, Chrysophyllum cainito L., UPLC QToF-MS/MS</jats:p>

Effect of Chrysophyllum cainito L. Leaves on Bone Formation In Vivo and In Silico

“…42 In silico study sample was metabolite profiling of 96% ethanol extract of C. cainito leaves with UPLC-QToF-MS/MS, as obtained from previous studies. 43 The screening of 41 compounds from the metabolite profiling analysis was done with SwissADME Webtool to know the physicochemical properties. 44 The compounds analysis program was PyRx 0.8 software with molecular docking and AutoDock Vina as a docking simulator.…”

Green Synthesis of MnO2 Nanoparticles with Aqueous Extract of Star Apple Leaves (Chrysophyllum cainito L.)

“…The aqueous extract of C. cainito leaves was analyzed quantitatively using UPLC-QToF-MS/MS to determine the component compounds contained thereinso we can confirm that the extract can used as chelating and reducing agent in the synthesis of MnO2. Before the analysis, the extract was first prepared using methanol solvent to optimize the separation of the compounds in the extract in order to obtain a spectrum with a higher sensitivity [19]. In addition, UPLC combined with multiple MS can increase the sensitivity and resolution of the analysis so that more accurate data is obtained, as well as increase the speed of analysis, so that it can take place in a shorter time than without a combination of instruments [19,20].…”

“…Before the analysis, the extract was first prepared using methanol solvent to optimize the separation of the compounds in the extract in order to obtain a spectrum with a higher sensitivity [19]. In addition, UPLC combined with multiple MS can increase the sensitivity and resolution of the analysis so that more accurate data is obtained, as well as increase the speed of analysis, so that it can take place in a shorter time than without a combination of instruments [19,20]. From the analysis process using UPLC-QToF -MS/MS, the chromatogram and MS data were obtained, as shown in Fig.…”

Efek Antiinflamasi Ekstrak dan Tablet Daun Kenitu (Chrysophyllum cainito L. ) pada Tikus Wistar (Rattus norvegicus)

“…Masyarakat Indonesia sering menggunakan daun C. cainito sebagai obat tradisional untuk mengatasi berbagai penyakit, antara lain radang saluran pernapasan, demam, dan diare (Ningsih et al, 2016). Selain itu, kandungan fitoestrogen pada daun C. cainito berpotensi digunakan sebagai alternatif pengobatan penyakit akibat defisiensi estrogen, seperti osteoporosis, penyakit neurodegeneratif dan penyakit kardiovaskular (Ma'arif et al, 2019)…”