Profile of metal-binding proteins and heme oxygenase in red carp treated with heavy metals, pesticides and surfactants

Ariyoshi, Toshihiko; Shiiba, Seiichi; Hasegawa, Hiroyuki; Arizono, Koji

doi:10.1007/bf01700889

Cited by 25 publications

(16 citation statements)

References 11 publications

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“…El Roundup®, así como el glifosato grado técnico y los surfactantes acompañantes, inducen una depresión de la función del citocromo P450, citocromo b5; de las enzimas SOD, catalasa (CAT) Glutation-S-transferasa y G-6-P deshidrogenasa, las cuales son vitales para el procesamiento de tóxicos en animales (Acquavella et al, 2004;Ariyoshi et al, 1990;Ariyoshi et al, 1991; Fan et al, 2013;Gehin et al, 2005;Costa et al, 2008; Elie-Caille et al., 2010; Modesto y Martínez, 2010) y puede ser una posible explicación a los cambios oxidativos evidenciados en este trabajo. No obstante, la actividad enzimática antioxidante no fue medida en el presente estudio.…”

Section: Explosión Respiratoriaunclassified

Efectos del Roundup® y Cosmoflux® 411f sobre la respuesta inmune frente al desafio con Aeromonas hydrophila en cachama blanca (Piaractus brachypomus)

et al. 2014

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ResumenLos herbicidas y pesticidas han mostrado afectar organismos no blancos de su acción. Dichos efectos incluyen alteración endocrina, así como teratogénesis, lesiones en sistema respiratorio, digestivo, inmune y nervioso. Particularmente, se ha documentado que las alteraciones del sistema inmune pueden generar efectos importantes sobre la capacidad del pez para sobrevivir en el medio. El presente trabajo evaluó el efecto de la exposición subletal al Roundup® (glifosato) y al surfactante acompañante (Cosmoflux® 411F) sobre la respuesta inmune al desafío bacteriano con Aeromonas hydrophila en cachama blanca (Piaractus brachypomus). Se evidenció que el Roundup®, así como la mezcla de este con el surfactante, disminuyen la resistencia de los animales al desafío con la bacteria y que el periodo de latencia es menor en los animales expuestos respecto al control. De la misma manera se evidenció un aumento de la actividad oxidativa en los animales expuestos. Los animales expuestos a las concentraciones subletales de glifosato, así como a la mezcla con el surfactante evidenciaron una mayor susceptibilidad frente a la infección experimental con A. hydrophila. Dichos efectos fueron mayores en el grupo expuesto a la mezcla, por lo cual se atribuye un efecto sinérgico ejercido por el surfactante en la misma.Palabras clave: cachama blanca, Cosmoflux® 411F, infección experimental, glifosato, inmunotoxicología, respuesta inmune Efectos del Roundup® y Cosmoflux® 411f sobre la respuesta inmune frente al desafio con Aeromonas hydrophila en cachama blanca (Piaractus brachypomus) 38 AbstractIt has been showed herbicides affect organisms which were not the target for their action. Such effects would include endocrine alteration, as well as teratogenesis and damage to the respiratory, digestive, and immune and nervous systems. It has been documented that alterations to the immune system could produce significant effects on a fish's ability to survive in its environment. The present work was aimed at evaluating the effect of sub-lethal exposure to Roundup® (glyphosate) and an accompanying surfactant (Cosmoflux® 411F) on the immune response to challenging white cachama/red-bellied pacu (Piaractus brachypomus) with the heterotrophic, Gram-negative, rod-shaped bacteria Aeromonas hydrophila. It was shown that Roundup®, as well as a mixture of this with the surfactant, reduced the animals' resistance to challenge with the bacteria and that the latency period was shorter in exposed animals regarding control. Likewise, increased oxidative activity was observed in the exposed animals. Animals exposed to sub-lethal concentrations of glyphosate, as well as the mixture with the surfactant, had greater susceptibility to experimental infection with A. hydrophila. Such effects were greater in the group exposed to the mixture, there by attributing a synergic effect to the surfactant. Key words:White cachama/red-bellied pacu, Cosmoflux® 411F, experimental infection, glyphosate, immunotoxicology, immune response. ResumoOs agro-toxicos tem demostra...

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Section: Explosión Respiratoriaunclassified

Efectos del Roundup® y Cosmoflux® 411f sobre la respuesta inmune frente al desafio con Aeromonas hydrophila en cachama blanca (Piaractus brachypomus)

et al. 2014

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“…Metallothioneins, for example, are low molecular mass, cysteine-rich metal-binding polypeptides that production can be induced by mercury exposure (Nordberg & Nordberg, 2000;Yoshida et al, 1999) and occur in a large number of evolutionarily diverse organisms, including fishes (George & Langston, 1994). Metallothioneins have an important role in essential metal homeostasis and in sequestering and detoxication of non-essential metals (Nordberg, 1998), as evidenced by the overexpression of these proteins in the target organs due to metal exposure, including in fish Cyprinus carpio (Ariyoshi et al, 1990).…”

Section: Introductionmentioning

confidence: 99%

Localization of Inorganic and Organic Mercury in the Liver and Kidney of Cyprinus carpio by Autometallography

Prodocimo

Neto²,

Grötzner³

et al. 2024

Ecotoxicol. Environ. Contam.

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Mercury bioaccumulation in different tissues largely depends upon metal chemical form and has important implications on tissue-specific toxicity. Then, simple tools for mercury tissue localization are very useful for understanding the toxicity of mercurial compounds in fishes. In the present study, inorganic and organic mercury distribution was investigated in carp Cyprinus carpio, utilizing autometallography. Juvenile C. carpio were exposed through force-feeding with mercury contaminated pelleted food at doses of 0.5 µg g -1 of Hg +2 or MeHg twice at 15 days interval, and killed 15 days after the second exposure for sampling. Organic mercury was observed inside hepatocytes and in biliary ducts of liver, whereas inorganic form of mercury was observed only in biliary ducts. In the kidney, mercury was observed mainly in renal tubules, with the predominance of inorganic over organic mercury. The present study demonstrated that autometallography is a useful tool for the tissue localization of two mercurial compounds in the liver and kidney of C. carpio after trophic exposure.Keywords: Autometallography, mercury, liver, kidney, Cyprinus carpio. Localização do mercúrio inorgânico e orgânico em fígado e rim de Cyprinus carpio através da autometalografia ResumoA bioacumulação de mercúrio em diferentes tecidos depende da forma química do metal e tem implicações importantes na toxicidade de tecidos específicos. Deste modo, ferramentas simples para a localização de mercúrio em tecidos são úteis para a compreensão da toxicidade de compostos mercuriais em peixes. No presente estudo, a distribuição de mercúrio inorgânico e orgânico foi investigada em carpa Cyprinus carpio, utilizando autometalografia. Exemplares juvenis de C. carpio foram expostos através de alimentação forçada com pellets de ração contaminada com mercúrio em doses de 0.5 µg g -1 Hg +2 ou MeHg duas vezes em um intervalo de quinze dias, e sacrificados 15 dias após a segunda exposição para amostragem. O mercúrio orgânico foi observado no interior dos hepatócitos e nos ductos biliares. No rim, o mercúrio foi observado principalmente nos túbulos renais, com predominância do mercúrio inorgânico sobre o orgânico. O presente estudo demonstrou que a autometalografia é uma ferramenta útil para a localização tecidual de dois compostos mercuriais em fígado e rim de C. carpio após exposição trófica.Palavras-chave: Autometalografia, mercúrio, fígado, rim, Cyprinus carpio.

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“…Cytochrome P-450's have shown excellent potential as biomarkers of PAH, PCB, and dioxin exposure (Melancon et al, 1987;Stegeman et al, 1987;Hahn et al, 1989). In addition, delta-aminolevulinic acid dehydratase activity (Hodson, et al, 1984), heme oxygenase (Ariyoshi et al, 1990;Mitani et al, 1990), and metallothionein (Jenkins et al, 1982;Sanders et al, 1983) have shown potential to serve as biomarkers of metal exposure.…”

Section: Introductionmentioning

confidence: 99%

“…For instance, synthesis of low molecular weight stress proteins, 20-40 kD, have been shown to be induced by oxidizing agents, arsenite, and heavy metal exposure (Shibahara et al, 1987;Keyse and Tyrell, 1989;Ariyoshi et al, 1990;Mitani et al, 1990). Thus, the SPR has potential to also serve as a chemical specific indicator.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of the Efficacy of the Stress Protein Response as a Biochemical Water Quality Biomonitoring Method

Dyer¹,

Dickson²,

Zimmerman³

1990

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To evaluate the potential use of the SPR as a biomonitoring tool, a stepwise plan was utilized that proceeded through various physical and chemical laboratory exposures and culminated with a field validation study. The goals of the laboratory exposures were threefold: 1) determine the time required for induction of the SPR; 2) determine the dose-responsiveness of the SPR; and 3) compare the increased syntheses and accumulations of stress proteins to classical toxicological endpoints (i.e. percent mortality, LC50, LC1, etc).Brain, gill, and striated muscle tissues of fathead minnows (Pimephales promelas) exposed to heat shock, sodium arsenite, sodium chromate, lindane, and diazinon elicited tissue and stressor specific translation patterns. Increased syntheses and accumulations of several proteins were found to be concentration dependent at environmentally relevant levels. All tissues under all exposure regimes elicited a 70 kD protein. This protein was determined to be indicative of general stress. Two proteins of 20 and 30 kD were only found in fish exposed to arsenite and chromate. Thus, these two proteins may serve as metal indicators.Gill of fathead minnows exposed in situ to a toxicity gradient in Soldier Creek, Midwest City, Oklahoma were found to accumulate stress protein 70 in a manner that reflected in stream fish species richness.The results of this research demonstrates that the stress protein response has potential as a rapid and environmentally relevant biomonitoring method for assessing both general and stressor specific perturbations in fish. Figure 3. A. A fluorogram of striated muscle protein from fathead minnows exposed to 34 0 C for 1-hour and then transferred to 25 0 C for 11 hours and sampled at 0, 1, 3, 7, and 11 hours post heat shock exposure. Molecular weight markers of 97, 66, 42, 31, 21, and 14 kD, respectively, are designated on the left. Arrows on the right denote heat shock proteins of 90, 78 and 70 kD. B. Median intensities of 90, 78, and 70 kD proteins in fluorograms from replicate samples (n = 3) of experiment described in A. Intensities were determined densito metrically and are presented as arbitrary units (see Materials and methods Unfortunately, their clinical use is lacking (Bouk, 1984). Clinical use implies that the method has been validated by extensive testing-both to identify the biological significance of the parameter and the limitations in applying the test to the species in question.Much of the early biomarker work involved measurements of blood and enzyme activity as well as biochemical composition of blood and tissues (Mazeud and Mazeud, 1981;Neff, 1985), methods primarily transplanted from human and veterinary medicine. Unfortunately, many of these parameters were not applicable across phyla and measured secondary effects, symptoms, of an underlying problem rather than highly conserved parameters intimately involved in the protection and/or defense of the cell. Thus, biomarkers that correspond to events that are coupled to cellular protection and defens...

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Profile of metal-binding proteins and heme oxygenase in red carp treated with heavy metals, pesticides and surfactants

Cited by 25 publications

References 11 publications

Efectos del Roundup® y Cosmoflux® 411f sobre la respuesta inmune frente al desafio con Aeromonas hydrophila en cachama blanca (Piaractus brachypomus)

Efectos del Roundup® y Cosmoflux® 411f sobre la respuesta inmune frente al desafio con Aeromonas hydrophila en cachama blanca (Piaractus brachypomus)

Localization of Inorganic and Organic Mercury in the Liver and Kidney of Cyprinus carpio by Autometallography

Evaluation of the Efficacy of the Stress Protein Response as a Biochemical Water Quality Biomonitoring Method

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