Profile of Multidrug Resistance and Methicillin-Resistant Staphylococcus aureus (MRSA) isolated from cats in Surabaya, Indonesia

Abstract

Abstract. Afnani DA, Fatih N, Effendi MH, Tyasningsih W, Kairullah AR, Kurniawan SC, Silaen OSM, Ramandianto SC, Widodo A, Hendriana K, Riwu KHP. 2022. Profile of Multidrug Resistance and Methicillin-Resistant Staphylococcus aureus (MRSA) isolated from cats in Surabaya, Indonesia. Biodiversitas 23: 5703-5709. Methicillin-Resistant Staphylococcus aureus (MRSA) is the name given to Staphylococcus aureus that has multidrug-resistance (MDR) characteristics and is resistant to ?-lactam drugs. Compared to other livestock, companion animals have been mentioned as potential MRSA reservoirs more frequently. This study aimed to identify MDR and detects MRSA strains from cats in Surabaya. A total of 150 nasal swab of cats were taken from several clinics and veterinary hospital. Samples were swabbed using Amies Medium Transport then identified using microbiological standard method. Kirby-Bauer diffusion method was performed for S. aureus antibiotic resistance profile on five different antibiotic discs. As a confirmatory test for MRSA, S. aureus isolates that were resistant to cefoxitin continued to grow on Oxacillin Resistance Screening Agar Base (ORSAB). The findings of the isolation and identification and process revealed 18 (12%) S. aureus isolates. The antibiotic resistance test revealed 3 (2%) MDR S. aureus isolates and 4 (2.6%) MDR S. aureus isolates that were ORSAB positive. It has been discovered that there are MDR and MRSA S. aureus isolates. It could be used as an indicator of irrational usage of antibiotics and it explained that cats could be source of infection to public health.

Detection of extended-spectrum β-lactamase-producing Escherichia coli genes isolated from cat rectal swabs at Surabaya Veterinary Hospital, Indonesia

“…Antibiotics are administered to animals at veterinary hospitals alone, in combination with other antibiotics, or in conjunction with chemotherapeutics [ 58 ]. Extended-spectrum β-lactamase-producing E. coli is among the bacteria that are frequently discovered to be resistant to β-lactam antibiotics, aminoglycosides, TEs, and C, which are frequently used in veterinary hospitals [ 59 ].…”

Detection mecA gene and Staphylococcus aureus resistance to several antibiotics isolated from cat ear swabs at a veterinary hospital located at Surabaya – Indonesia

“…These findings align with those of Waruwu et al (2023), who discovered higher MDR S. aureus infections in cats at a rate of 19.51% (16/82). Afnani et al (2022) found a 38.89% (7/18) MDR of S. aureus in cats. This could account for the three isolates from 100 cat nose swab samples representing Surabaya's remaining low-MDR S. aureus infections.…”

“…It is recognized that S. aureus bacteria can spread from pets to people or vice-versa (Afnani et al, 2022). Given that pets are frequently treated as family members, it is impractical to enable physical interaction between people and animals, including skin-to-skin contact, aerosols from coughing, sneezing, and saliva (Damborg et al, 2016).…”

Kinship analysis of mecA gene of methicillin-resistant Staphylococcus aureus isolated from milk and risk factors from the farmers in Blitar, Indonesia

“…First, a milk sample and farmer's hand swab were taken using a loop, then spread in a zigzag manner Available at www.veterinaryworld.org/Vol.17/January-2024/25.pdf on NA media (Oxoid, UK) and incubated for 24 h at 37°C. Bacterial isolates grown on nutrient agar media were collected using a loop, streaked in a zigzag manner on mannitol salt agar (MSA) media (Oxoid), and incubated for 24 h at 37°C [27]. Suspected S. aureus colonies (golden-yellow) were further characterized using standard microbiology techniques, such as Gram staining, coagulase, catalase, β-galactosidase, pyrrolidonyl arylamidase, and acetoin production tests [28].…”