Profile of Progesteron, Estrogen and Pregnancy Level of Pasundan Cattle after Estrus Synchronization with Prostaglandin and Releasing Gonadothropin Hormone

Abstract

Twenty Pasundan cows were used in this study in order to determine the concentration of the hormones estrogen, progesterone, and the level of pregnancy. The cows were randomly divided into two groups of 10 each. The first group was synchronized with a prostaglandin double injection (Lutalyse TM, Upjohn, Kalmozoo USA, containing 25 mg of Dinoprost Tromethamin) at a dose of 5 ml/head intramuscularly 2 (two) times with an interval of 11 days. The second group was injected with prostaglandins (PGF2α) at a dose of 5 ml/head intramuscularly 2 (two) times with an interval of 11 days, but on the 9th day they were injected with gonadotropin realizing hormone (Fertagyl, Intervet Animal Health UK Ltd, Cambridge; which contained 100 µg Gonadorelin which is synthetic GnRH)), Cows in heat are artificially inseminated twice with an interval of 6 hours. The variables observed were concentrations of estrogen and progesterone during estrus and 2 months of pregnancy, service preconception, and calving rate. The data were processed with analysis of variance and descriptive. The results showed that cows that were injected with PGF2 α + GnRH, had an average plasma concentration of the hormone progesterone 2.68 ± 0.19 ng/ml and 26.65 ± 2.09 pg/ml estrogen, service preconception 1.0, a calving rate 90% higher than those injected with α PGF2 including progesterone. 2.68 ± 0.19 ng / ml and estrogen 26.65 ± 2.09 pg / ml, service preconception 1.2 and calving rate 90%. The results of the analysis of variance showed that the plasma concentrations of progesterone and estrogen when the cows were 60 days pregnant when they were injected with the PGF2α + GnRH combination had a very significant effect (P <0.01) higher than the control (PGF2α). It was concluded that the progesterone, estrogen profile, pregnancy rate results from estrus synchronization using a combination of PGF2 α and HnRH higher than single PGF2α.