Profiles and morphology of peripheral blood cells in three bat species of Karelia during hibernation

Uzenbaeva, L. B.; Belkin, Vladimir; Ilyukha, V. A.; Kizhina, A. G.; Yakimova, Alina

doi:10.1134/s0022093015040109

Cited by 6 publications

(2 citation statements)

References 9 publications

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“…However, in some lipopolysaccharide (LPS)-stimulated insectivores, no leukocytosis or fever was observed [ 48 ]. Another feature of leukocyte kinetics reported in Vespertilionidae bats in Karelia is the clear leukopenia generated during hibernation in bats [ 49 ]. These authors concluded that the differences in the proportion of lymphocytes may be related to the biological, ecological, and physiological peculiarities of the bats studied, which shows the need for further studies to determine a correlation between bat infection with ZIKV and leukocyte kinetics of Artibeus .…”

Section: Discussionmentioning

confidence: 99%

Experimental infection of Artibeus lituratus bats and no detection of Zika virus in neotropical bats from French Guiana, Peru, and Costa Rica suggests a limited role of bats in Zika transmission

Aguilar-Setién,

Salas-Rojas,

Gálvez-Romero

et al. 2023

PLoS Negl Trop Dis

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Bats are important natural reservoir hosts of a diverse range of viruses that can be transmitted to humans and have been suggested to play an important role in the Zika virus (ZIKV) transmission cycle. However, the exact role of these animals as reservoirs for flaviviruses is still controversial. To further expand our understanding of the role of bats in the ZIKV transmission cycle in Latin America, we carried out an experimental infection in wild-caught Artibeus lituratus bats and sampled several free-living neotropical bats across three countries of the region. Experimental ZIKV infection was performed in wild-caught adult bats (4 females and 5 males). The most relevant findings were hemorrhages in the bladder, stomach and patagium. Significant histological findings included inflammatory infiltrate consisting of a predominance of neutrophils and lymphocytes, in addition to degeneration in the reproductive tract of males and females. This suggests that bat reproduction might be at some level affected by ZIKV. Leukopenia was also observed in some inoculated animals. Hemorrhages, genital alterations, and leukopenia are suggested to be caused by ZIKV; however, since these were wild-caught bats, we cannot exclude other agents. Detection of ZIKV by qPCR was observed at low concentrations in only two urine samples in two inoculated animals. All other animals and tissues tested were negative. Finally, no virus-neutralizing antibodies were found in any animal. To determine ZIKV infection in nature, the blood of a total of 2056 bats was sampled for ZIKV detection by qPCR. Most of the sampled individuals belonged to the genus Pteronotus sp. (23%), followed by the species Carollia sp. (17%), Anoura sp. (14%), and Molossus sp. (13.7%). No sample of any tested species was positive for ZIKV by qPCR. These results together suggest that bats are not efficient amplifiers or reservoirs of ZIKV and may not have an important role in ZIKV transmission dynamics.

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Section: Discussionmentioning

confidence: 99%

Experimental infection of Artibeus lituratus bats and no detection of Zika virus in neotropical bats from French Guiana, Peru, and Costa Rica suggests a limited role of bats in Zika transmission

Aguilar-Setién,

Salas-Rojas,

Gálvez-Romero

et al. 2023

PLoS Negl Trop Dis

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“…Usually, 4-5 types of WBCs are present in bats' blood smears: Lymphocytes, monocytes, neutrophils, eosinophils, and sometimes basophils [22,26,27]. Additional types of WBC were observed in some studies [21,28,29]. Some researchers found it difficult to stain the bat blood smears in field investigations.…”

Section: Discussionmentioning

confidence: 99%

Comparative staining of Rhinolophus spp. white blood cells in blood smears

et al. 2022

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Background and Aim: A drawback of studies on bat blood smears in the field is the lack of time for fixation because blood sampling using a non-lethal method often provides less time for fixation in smear preparations due to the small volume of blood collected. Usually, there is insufficient blood for another smear preparation, so it is necessary to use blood smears as rationally as possible, especially for rare bats. Many stains are used for staining peripheral blood smears, and they have advantages and disadvantages. This study aimed to examine commonly used stains for blood smears to select the best stain for staining peripheral blood smears in bats. Materials and Methods: In this study, 48 blood smears of Rhinolophus spp. bats were examined using several blood staining methods. Four methods that showed the best results were used in further experiments: Romanowsky-Giemsa, Pappenheim, hematoxylin-eosin, and eosin methylene blue. Results: Comparative analysis of different methods for staining bat blood smears revealed that the most convenient method for analyzing blood cells is Pappenheim method. Conclusion: Staining blood smears using Pappenheim method yield the least number of unsuccessful blood smear stains and are quite effective for the morphological analysis of blood cells.

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