Profiles of antioxidant gene expression and physiological changes by thermal and hypoosmotic stresses in black porgy (Acanthopagrus schlegeli)

An, Kwang Wook; Kim, Na Na; Shin, Hyun Suk; Kil, Gyung‐Suk; Choi, Cheol Young

doi:10.1016/j.cbpa.2010.02.013

Cited by 46 publications

(17 citation statements)

References 33 publications

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“…To date, no study has been conducted on the intracorporeal oxidative stress due to low temperature. ROS or oxygen free radicals seem to be produced in the body because of oxidative stress when marine organisms are exposed to temperatures other than their normal habitat temperature, and the expression and activation of antioxidant enzyme genes subsequently increase to eliminate these oxygen free radicals (Abele et al 1998;An et al 2010). Although no deaths were recorded during the experiment, the results led to the speculation that cinnamon clownfish continuously experience oxidative stress in hypoosmotic or low-temperature environments.…”

Section: Discussionmentioning

confidence: 95%

“…The reason for this is that cinnamon clownfish, which live in subtropical environments, experience stress when exposed to low temperatures, whereby oxidative stress also increases. Although previous studies have examined antioxidant enzyme gene expression under different water temperatures, they included fish such as goldfish and olive flounder that were exposed to high-temperature environments (Bagnyukova et al 2007;An et al 2010;Shin et al 2010a). To date, no study has been conducted on the intracorporeal oxidative stress due to low temperature.…”

Section: Discussionmentioning

confidence: 99%

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Effect of hypoosmotic and thermal stress on gene expression and the activity of antioxidant enzymes in the cinnamon clownfish,Amphiprion melanopus

Park

Shin

Choi

et al. 2011

Animal Cells and Systems

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We studied oxidative stress in cinnamon clownfish exposed to hypoosmotic (35 psu 0 17.5 psu and 17.5 psu with prolactin (PRL)) and low temperature (288C 0 248C and 208C) conditions by measuring the expression and activity of Cu/Zn-superoxide dismutase (Cu/Zn-SOD), catalase (CAT), and glutathione peroxidase (GPX). The expression and activity of the antioxidant enzymes were significantly higher after the fish were exposed to 248C, 208C, and 17.5 psu, and expression was repressed by PRL treatment. Furthermore, we measured H 2 O 2 and lipid peroxidation levels and found that they were significantly higher after exposure to the hypoosmotic and low-temperature environments. Additionally, we investigated changes in plasma AST and ALT levels after exposure to low temperature and hypoosmotic stress. These levels increased upon exposure of the clownfish to 248C, 208C, and 17.5 psu, but the levels of these parameters decreased in the 17.5 psu with PRL treatment during a salinity change. The results indicate that hypoosmotic and low-temperature conditions induce oxidative stress in cinnamon clownfish and that the parameters tested in this study may be indices of oxidative stress in the cinnamon clownfish.

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Effect of hypoosmotic and thermal stress on gene expression and the activity of antioxidant enzymes in the cinnamon clownfish,Amphiprion melanopus

Park

Shin

Choi

et al. 2011

Animal Cells and Systems

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“…Thus, temperature-induced oxidative stress appears to be widespread. Increases in tissue oxidative damage following challenges with either acute cold or acute heat has been observed in a variety of taxa (marine: Parihar et al, 1996;Heise et al, 2006;Bagnyukova et al, 2007;An et al, 2010;Ibarz et al, 2010;Castro et al, 2012;Rosa et al, 2012;birds: Mujahid and Furuse, 2009;Constantini et al, 2012;insects: Lalouette et al, 2011;reptiles: Ballen et al, 2012). Changes in enzymatic antioxidant production and activity during thermal stress also occur, but are highly variable and context dependent (Constantini, 2014).…”

Section: Introductionmentioning

confidence: 99%

Temperature experienced during incubation affects antioxidant capacity but not oxidative damage in hatchling red-eared slider turtles (Trachemys scripta elegans)

Treidel

Carter

Bowden

2015

Journal of Experimental Biology

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Our understanding of how oxidative stress resistance phenotypes are affected by the developmental environment is limited. One component of the developmental environment, which is likely central to early life oxidative stress among ectothermic and oviparous species, is that of temperature. We investigated how incubation temperature manipulations affect oxidative damage and total antioxidant capacity (TAC) in red-eared slider turtle (Trachemys scripta elegans) hatchlings. First, to determine whether temperature fluctuations elicit oxidative stress, eggs from clutches were randomly assigned to either a constant (29.5°C) or daily fluctuating temperature incubation (28.7±3°C) treatment. Second, to assess the effect of temperature fluctuation frequency on oxidative stress, eggs were incubated in one of three fluctuating incubation regimes: 28.7±3°C fluctuations every 12 h (hyper), 24 h (normal) or 48 h (hypo). Third, we tested the influence of average incubation temperature by incubating eggs in a daily fluctuating incubation temperature regime with a mean temperature of 26.5°C (low), 27.1°C (medium) or 27.7°C (high). Although the accumulation of oxidative damage in hatchlings was unaffected by any thermal manipulation, TAC was affected by both temperature fluctuation frequency and average incubation temperature. Individuals incubated with a low frequency of temperature fluctuations had reduced TAC, while incubation at a lower average temperature was associated with enhanced TAC. These results indicate that although sufficient to prevent oxidative damage, TAC is influenced by developmental thermal environments, potentially because of temperature-mediated changes in metabolic rate. The observed differences in TAC may have important future consequences for hatchling fitness and overwinter survival.

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“…It is mainly referred to as the main biomarker of stress, which leads to oxidative changes [6]. However, a correlation between plasma hydrocortisone and Cu/Zn SOD activity has been recorded in rhesus monkeys, and both have been found to have a similar diurnal rhythm [7].…”

Section: Introductionmentioning

confidence: 99%

The effect of indomethacin, myeloperoxidase, and certain steroid hormones on bactericidal activity: an ex vivo and in vivo experimental study

Stark

Varga

Ghidán

et al. 2014

Ann Clin Microbiol Antimicrob

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BackgroundThe role of myeloperoxidase (MPO) is essential in the killing of phagocytosed bacteria. Certain steroid hormones increase MPO plasma concentration. Our aim was to test the effect of MPO, its inhibitor indomethacin, and certain steroid hormones on bactericidal activity.MethodsHuman polymorphonuclear leukocytes (PMN) were incubated with opsonised Escherichia coli and either MPO, indomethacin, estradiol, or hydrocortisone. Intracellular killing capacity was evaluated with UV microscopy after treatment with fluorescent dye. Next, an in vivo experiment was performed with nine groups of rats: in the first phase of the study indomethacin treatment and Pasteurella multocida infection (Ii), indomethacin treatment without infection (I0), untreated control with infection (Mi) and untreated control without infection (M0); in the second phase of the study rats with infection and testosterone treatment (NT), castration, infection and testosterone treatment (CT), castration, infection and estradiol treatment (CE), non-castrated infected control (N0), and castrated infected control (C0). After treatment bacteria were reisolated from the liver and heart blood on agar plates, and laboratory parameters were analyzed. For the comparison of laboratory results ANOVA or Kruskal-Wallis test and LSD post hoc test was used.ResultsIndomethacin did not have a remarkable effect on the bacterial killing of PMNs, while the other compounds increased bacterial killing to various degrees. In the animal model indomethacin and infection caused a poor clinical state, a great number of reisolated bacteria, elevated white blood cell (WBC) count, decreased C-reactive protein (CRP) and serum albumin levels. Testosterone treatment resulted in less bacterial colony numbers in group NT, but not in group CT compared to respective controls (N0, C0). Estradiol treatment (CE) decreased colony numbers compared to control (C0). Hormone administration resulted in lower WBC counts, and in group CE, a decreased CRP.ConclusionsMPO, estradiol, and hydrocortisone improve bacterial killing activity of PMNs. Indomethacin treatment and castration weaken immune responses and clinical state of infected rats, while testosterone and estradiol have a beneficial effect.

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Profiles of antioxidant gene expression and physiological changes by thermal and hypoosmotic stresses in black porgy (Acanthopagrus schlegeli)

Cited by 46 publications

References 33 publications

Effect of hypoosmotic and thermal stress on gene expression and the activity of antioxidant enzymes in the cinnamon clownfish,Amphiprion melanopus

Effect of hypoosmotic and thermal stress on gene expression and the activity of antioxidant enzymes in the cinnamon clownfish,Amphiprion melanopus

Temperature experienced during incubation affects antioxidant capacity but not oxidative damage in hatchling red-eared slider turtles (Trachemys scripta elegans)

The effect of indomethacin, myeloperoxidase, and certain steroid hormones on bactericidal activity: an ex vivo and in vivo experimental study

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