Profiles of fragments after pulsed-field gel electrophoresis of cleaved genomic DNA from strains of Taylorella equigenitalis isolated from horses in Norway

Matsuda, Motoo; Miyazawa, T.; Ishida, Yasumasa; Moore, John E.

doi:10.1016/s0944-5013(97)80015-8

Cited by 7 publications

(7 citation statements)

References 11 publications

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“…In particular, the distinct genotypes between the strain NCTC11184 T and Kentucky188 were clearly demonstrated by the PFGE pro¢les as described previously (Matsuda et al, 1998(Matsuda et al, , 2000. The present results showing only minor sequence di¡erences between the ISRs of the strains of NCTC11184 T and Kentucky188 suggest that the ISRs could be similarly useful for the discrimination among isolates of T. equigenitalis by the PFGE procedure if the procedure of sequencing ISRs is employed.…”

Section: Discussionsupporting

confidence: 85%

Nucleotide Sequencing and Analysis of 16S rDNA and 16S-23S rDNA Internal Spacer Region (ISR) of Taylorella equigenitalis, as an Important Pathogen for Contagious Equine Metritis (CEM)

et al. 2006

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The primer set for 16S rDNA amplified an amplicon of about 1500 bp in length for three strains of Taylorella equigenitalis (NCTC11184(T), Kentucky188 and EQ59). Sequence differences of the 16S rDNA among the six sequences, including three reference sequences, occurred at only a few nucleotide positions and thus, an extremely high sequence similarity of the 16S rDNA was first demonstrated among the six sequences. In addition, the primer set for 16S-23S rDNA internal spacer region (ISR) amplified two amplicons about 1300 bp and 1200 bp in length for the three strains. The ISRs were estimated to be about 920 bp in length for large ISR-A and about 830 bp for small ISR-B. Sequence alignment of the ISR-A and ISR-B demonstrated about 10 base differences between NCTC11184(T) and EQ59 and between Kentucky188 and EQ59. However, only minor sequence differences were demonstrated between the ISR-A and ISR-B from NCTC11184(T) and Kentucky188, respectively. A typical order of the intercistronic tRNAs with the 29 nucleotide spacer of 5'-16S rDNA-tRNA(Ile)-tRNA(Ala)-23S rDNA-3' was demonstrated in the all ISRs. The ISRs may be useful for the discrimination amongst isolates of T. equigenitalis if sequencing is employed.

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Section: Discussionsupporting

confidence: 85%

Nucleotide Sequencing and Analysis of 16S rDNA and 16S-23S rDNA Internal Spacer Region (ISR) of Taylorella equigenitalis, as an Important Pathogen for Contagious Equine Metritis (CEM)

et al. 2006

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“…These genotypic and phenotypic data, coupled with epidemiological information, confirmed relationships between strains isolated from horses with positive test results within each CEM incident in the United States and also showed a lack of strain relationships between CEM incidents. This study and previous studies (2,8,9,10,11,12,13,16) have demonstrated that PFGE is valuable in discriminating strains of T. equigenitalis. Isolates from horses with positive test results in U.S. outbreaks and isolates from imported horses with positive test results were tested using PFGE, resulting in 15 distinct genotypes.…”

Section: Discussionsupporting

confidence: 69%

“…Testing of 18 strains isolated in Ireland identified three distinct genotypes, and tests of 8 U.S. strains indicated four distinct genotypes (12). In Norway, the profiles of eight strains of T. equigenitalis were indistinguishable (13). Testing of field strains of T. equigenitalis isolated between 1979 and 1980 in Australia produced three different genotypes (10).…”

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confidence: 96%

“…Molecular genotyping of T. equigenitalis strains by the use of pulsed-field gel electrophoresis (PFGE) or crossed-field gel electrophoresis (CFGE), a variation of PFGE, has been previously performed on isolates from Europe (8), Japan (11,16), Ireland and the United States (12), Norway (13), and Australia (10) by the use of restriction enzymes ApaI and NotI. In Europe, 46 isolates of T. equigenitalis from six countries were classified into 18 genotypes (8).…”

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confidence: 99%

“…In Japan, testing of 130 strains of T. equigenitalis isolated between 1980 and 1996 identified only a single genotype (11,16). Testing of 18 strains isolated in Ireland identified three distinct genotypes, and tests of 8 U.S. strains indicated four distinct genotypes (12). In Norway, the profiles of eight strains of T. equigenitalis were indistinguishable (13).…”

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confidence: 99%

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Pulsed-Field Gel Electrophoresis Genotyping of Taylorella equigenitalis Isolates Collected in the United States from 1978 to 2010

Aalsburg¹,

Erdman²

2011

J Clin Microbiol

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Taylorella equigenitalis is the etiologic agent of contagious equine metritis (CEM), a venereal disease of horses. A total of 82 strains of T. equigenitalis isolated in the United States were analyzed by pulsed-field gel electrophoresis (PFGE) after digestion of genomic DNA with restriction enzyme ApaI. Twenty-eight of those strains isolated from horses in the 2009 U.S. outbreak (CEM09) were further analyzed with NotI and NaeI enzymes. When ApaI alone was used for analysis, the 82 isolates clustered into 15 different genotypes that clearly defined groups of horses with known epidemiological connections. The PFGE profiles of the CEM09 isolates were indistinguishable after digestion with ApaI, NotI, and NaeI and did not match those of isolates from previous U.S. outbreaks in 1978 and 2006 or of any other isolate from the National Veterinary Services Laboratories (NVSL) culture library. Coupled with the fact that the CEM09 isolates are epidemiologically related, these results suggest a common source for the outbreak not linked to previous occurrences of CEM in the United States.

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Genotyping of German and Austrian Taylorella equigenitalis isolates using repetitive extragenic palindromic (REP) PCR and pulsed-field gel electrophoresis (PFGE)

Sting

Seeh

Mauder

et al. 2016

Research in Veterinary Science

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Profiles of fragments after pulsed-field gel electrophoresis of cleaved genomic DNA from strains of Taylorella equigenitalis isolated from horses in Norway

Cited by 7 publications

References 11 publications

Nucleotide Sequencing and Analysis of 16S rDNA and 16S-23S rDNA Internal Spacer Region (ISR) of Taylorella equigenitalis, as an Important Pathogen for Contagious Equine Metritis (CEM)

Nucleotide Sequencing and Analysis of 16S rDNA and 16S-23S rDNA Internal Spacer Region (ISR) of Taylorella equigenitalis, as an Important Pathogen for Contagious Equine Metritis (CEM)

Pulsed-Field Gel Electrophoresis Genotyping of Taylorella equigenitalis Isolates Collected in the United States from 1978 to 2010

Genotyping of German and Austrian Taylorella equigenitalis isolates using repetitive extragenic palindromic (REP) PCR and pulsed-field gel electrophoresis (PFGE)

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