Profiles of Gonadal Development, Sex Steroids, Aromatase Activity, and Gonadotropin II in the Controlled Sex Change of Protandrous Black Porgy, Acanthopagrus schlegeli Bleeker

Lee, Yan‐Horn; Lee, Fang-Yi; Yueh, Wen-Shiun; Tacon, Philippe; Du, Jin-Lien; Chang, Cheng‐Nan; Jeng, Shan-Ru; Tanaka, Hideki; Chang, Ching‐Fong

doi:10.1006/gcen.2000.7499

Cited by 76 publications

(65 citation statements)

References 24 publications

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“…Correlative support for a role of E 2 in sex change has been revealed in numerous hermaphroditic fish species (Devlin & Nagahama 2002, and references therein; Kroon & Liley 2000;Lee et al 2001;Godwin et al 2003;Kroon et al 2003). E 2 administration has induced male-to-female sex change in some species (Devlin & Nagahama 2002, and references therein; Lee et al 2000Lee et al , 2001, while the administration of an aromatase inhibitor induced sex change in a protogynous goby (Kroon & Liley 2000) and grouper (Bhandari et al 2004), but blocked natural sex change in a protandrous porgy (Lee et al 2001). Moreover, elevated aromatase activity in the brain and gonads was associated with maleto-female sex change in this porgy (Chang & Lin 1998;Lee et al 2000).…”

Section: Introductionmentioning

confidence: 94%

“…E 2 administration has induced male-to-female sex change in some species (Devlin & Nagahama 2002, and references therein; Lee et al 2000Lee et al , 2001, while the administration of an aromatase inhibitor induced sex change in a protogynous goby (Kroon & Liley 2000) and grouper (Bhandari et al 2004), but blocked natural sex change in a protandrous porgy (Lee et al 2001). Moreover, elevated aromatase activity in the brain and gonads was associated with maleto-female sex change in this porgy (Chang & Lin 1998;Lee et al 2000). Thus, it appears that the aromatase pathway is involved in mediating sex change in hermaphroditic fishes, possibly by modulating E 2 concentrations that help control gonadal cell allocation.…”

Section: Introductionmentioning

confidence: 99%

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Aromatase pathway mediates sex change in each direction

et al. 2005

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The enzyme aromatase controls the androgen/oestrogen ratio by catalysing the irreversible conversion of testosterone into oestradiol (E 2 ). Therefore, the regulation of E 2 synthesis by aromatase is thought to be critical in sexual development and differentiation. Here, we demonstrate for the first time that experimental manipulation of E 2 levels via the aromatase pathway induces adult sex change in each direction in a hermaphroditic fish that naturally exhibits bidirectional sex change. Our results demonstrate that a single enzymatic pathway can regulate both female and male sexual differentiation, and that aromatase may be the key enzyme that transduces environmental, including social, cues to functional sex differentiation in species with environmental sex determination.

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Section: Introductionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Aromatase pathway mediates sex change in each direction

et al. 2005

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“…On the contrary, hormones and environmental factors play very important roles. Among these factors, estrogen plays key roles in ovarian differentiation in non-mammalian vertebrates, including fish (Chang et al 1997, Kwon et al 2000, Lee et al 2000, Kobayashi et al 2003, amphibians (Yu et al 1993), and birds (Elbrecht & Smith 1992, Smith & Sinclair 2004. As the key enzyme catalyzing the conversion of endogenous androgen to estrogen, aromatase is highly related to gonad malformation, sterility, intersex, or sex reversal.…”

Section: Regulatory Action Of Estrogen On Dmrt1s Foxl2 and Cyp19s Imentioning

confidence: 99%

Molecular cloning of doublesex and mab-3-related transcription factor 1, forkhead transcription factor gene 2, and two types of cytochrome P450 aromatase in Southern catfish and their possible roles in sex differentiation

Liu¹,

Wu²,

Jiao³

et al. 2007

Journal of Endocrinology

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To address the roles of doublesex and mab-3-related transcription factor 1 (Dmrt1), forkhead transcription factor gene 2 (Foxl2), and aromatase in sex differentiation of Southern catfish, the cDNA sequences of these genes were isolated from the gonads. Dmrt1a and Dmrt1b were found to be expressed in the gonads, being higher in the testis. A low expression level of Dmrt1b was also detected in the intestine and kidney of the male. Foxl2 was found to be expressed extensively in the brain (B), pituitary (P), gill and gonads (G), with the highest level in the ovary, indicating the possible involvement of Foxl2 in the B-P-G axis. Cytochrome P450 (Cyp)19b was found to be expressed in the brain, spleen, and gonads, while Cyp19a was only expressed in the gonads and spleen. All-female Southern catfish fry were treated with fadrozole (F), tamoxifen (TAM), and 17b-estradiol (E 2 ) respectively, from 5 to 25 days after hatching (dah).The expression levels of these genes were measured at 65 dah. In the F-, TAM-, and FCTAM-treated groups, Dmrt1a and Dmrt1b were up-regulated in the gonad, whereas Foxl2 and Cyp19a were down-regulated, while the expression of Cyp19b in the gonad remained unchanged. Furthermore, downregulation of Foxl2 and Cyp19b was also detected in the brain.In the E 2 -treated group, Dmrt1a and Dmrt1b were downregulated to an undetectable level in the gonad, whereas Foxl2 and Cyp19b were up-regulated in the brain. Consistent with the observed changes in the expressions of these genes, 56, 70, and 80% sex-reversed male individuals were obtained in the F-, TAM-, and FCTAM-treated groups respectively. These results indicate the significant roles of Dmrt1, Foxl2, and Cyp19 in the sex differentiation of Southern catfish.

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“…In the sequential hermaphrodite fish, sex change, the transformation of an individual from one sex to the other in adulthood, occurs naturally in its life cycle. It has been shown that estradiol (E 2 ) administration induced male to female sex change of protandrous black porgy (Lee et al 2000(Lee et al , 2001, while inhibition of aromatase, the enzyme complex for catalyzing the conversion of androgens to estrogens, induced sex change from female to male in the protogynous goby (Kroon & Liley 2000) and grouper (Bhandari et al 2004), but blocked natural sex change in the protandrous porgy (Lee et al 2001). In Gobiodon erythrospilus, a hermaphroditic fish that naturally exhibits bidirectional sex change, manipulation of E 2 levels via the aromatase pathway induced adult sex change in each direction (Kroon et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Two cytochrome P450 aromatase genes in the hermaphrodite ricefield eel Monopterus albus: mRNA expression during ovarian development and sex change

Zhang

Yang

et al. 2008

Journal of Endocrinology

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Previously, the ricefield eel (Monopterus albus) was speculated to have only one cytochrome p450 aromatase gene. In this study, however, the cDNAs encoding two distinct cytochrome p450 aromatases, cyp19a1a and cyp19a1b, were isolated. The genomic organizations of both cyp19 genes were conserved when compared with other teleosts. Northern blot detected an abundant expression of cyp19a1a in the ovary, and cyp19a1b in the hypothalamus. RT-PCR coupled with Southern blot showed that cyp19a1a was expressed predominantly in the gonads of both sexes, with higher levels in the ovary than testis, while cyp19a1b was expressed in all the tissues examined in the male, but only in the brain and pituitary in the female. The levels of cyp19a1a mRNA in the ovary were increased significantly during vitellogenesis, but decreased significantly at mature stage. The levels of cyp19a1b mRNA in the brain and pituitary did not vary significantly during vitellogenesis. As ovarian development shifted from vitellogenesis to maturation, the levels of cyp19a1b mRNA was decreased significantly in the brain, but increased significantly in the pituitary. During natural sex change from female to male, the levels of cyp19a1a mRNA in the gonad were significantly decreased. The levels of cyp19a1b mRNA in the hypothalamus were significantly increased at the early intersexual phase, whereas the expression levels in the pituitary were significantly decreased at the intersexual phases. Taken together, these results showed a novel sexual dimorphism of cyp19a1b mRNA tissue distribution, and both CYP19 genes were associated with the ovarian development and natural sex change of the ricefield eel.

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Profiles of Gonadal Development, Sex Steroids, Aromatase Activity, and Gonadotropin II in the Controlled Sex Change of Protandrous Black Porgy, Acanthopagrus schlegeli Bleeker

Cited by 76 publications

References 24 publications

Aromatase pathway mediates sex change in each direction

Aromatase pathway mediates sex change in each direction

Molecular cloning of doublesex and mab-3-related transcription factor 1, forkhead transcription factor gene 2, and two types of cytochrome P450 aromatase in Southern catfish and their possible roles in sex differentiation

Two cytochrome P450 aromatase genes in the hermaphrodite ricefield eel Monopterus albus: mRNA expression during ovarian development and sex change

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