Profiles of Insulin-Like Growth Factor Binding Proteins and the Protease Activity in the Maternal Circulation and Its Local Regulation Between Placenta and Decidua.

Sakai, Kiyofumi; Iwashita, Mitsutoshi; Takeda, Yoshihiko

doi:10.1507/endocrj.44.409

Cited by 21 publications

(17 citation statements)

References 35 publications

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“…IGFBP-3 protease activity largely disappears after parturition suggesting that much of IGFBP-3 protease arises from the placenta, decidual tissue, or trophoblasts (8,22,23). In late pregnancy serum, protease activity directed against IGFBP-3 is present in fractions containing proteins of 50 -100 kDa (5).…”

Section: Discussionmentioning

confidence: 99%

ADAM 12, a Disintegrin Metalloprotease, Interacts with Insulin-like Growth Factor-binding Protein-3

Shi¹,

Xu²,

Loechel³

et al. 2000

Journal of Biological Chemistry

178

128

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Insulin-like growth factor-binding protein (IGFBP)-3 binds the insulin-like growth factors with high affinity and modulates their actions. Proteolytic cleavage of IG-FBP-3 may regulate insulin-like growth factor bioavailability. IGFBP-3 is extensively degraded in serum during pregnancy; however, as yet the pregnancy-specific protease, or proteases, have not been identified. We utilized a yeast two-hybrid assay and a human placental cDNA library to investigate IGFBP-3-interacting proteins. A disintegrin and metalloprotease-12 (ADAM 12), a member of a family of metalloprotease disintegrins that is highly expressed in placental tissue, was identified as interacting with IGFBP-3. This interaction involved the cysteine-rich domain of ADAM 12. Unlike other members of this family of disintegrin metalloproteases that are membrane proteins, ADAM 12 exists as an alternatively spliced soluble secreted protein. To verify the interaction between ADAM 12 and IGFBP-3, an expression construct containing an ADAM 12-S cDNA was transfected into COS-1 cells. Co-precipitation was observed when conditioned medium was analyzed by immunoprecipitation with an antibody against either ADAM 12 or IGFBP-3 followed by Western blotting with anti-IG-FBP-3 or anti-ADAM 12. Although minimal proteolysis of IGFBP-3 was observed in conditioned medium from control cells, this was increased ϳ4-fold in conditioned medium from ADAM 12-S-transfected cells. Recombinant ADAM 12-S partially purified from conditioned medium on a heparin-Sepharose column also proteolyzed IG-FBP-3. The degradation pattern was similar to that seen with pregnancy serum, and the presence of ADAM 12-S in serum during pregnancy was confirmed. The data suggest that ADAM 12-S has IGFBP-3 protease activity, and it may contribute to the IGFBP-3 protease activity present in pregnancy serum.

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Section: Discussionmentioning

confidence: 99%

ADAM 12, a Disintegrin Metalloprotease, Interacts with Insulin-like Growth Factor-binding Protein-3

Shi¹,

Xu²,

Loechel³

et al. 2000

Journal of Biological Chemistry

178

128

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“…In non-mammalian species, IGF-II does not bind to the mannose-6-phosphate receptor (Clairmont & Czech 1989). In the human, both type I and type II receptors have been identified in a wide range of tissues including ovarian follicles (Hernandez et al 1992), the endometrium (Ghahary & Murphy 1989) and the placenta (Han et al 1996, Sakai et al 1997. However, fetal tissues have greater type I and type II IGF binding activities than adult tissues (Ocrant et al 1988).…”

Section: Igfs and Igfbpsmentioning

confidence: 99%

IGFs and IGF-binding proteins in the regulation of human ovarian and endometrial function

Hs¹,

Chard²

1999

Journal of Endocrinology

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“…The serum concentrations of IGFBP-3 increase gradually during pregnancy [4, 10], while those of IGFBP-1 are increased in the first trimester and remain high until delivery [4, 10, 12, 13]. In addition, during pregnancy, IGFBP-3 is degraded by proteases to a considerable extent [13,14,15], possibly produced by the decidua [14]. It is conceivable that this results in a decreased affinity of IGFBP-3 for IGF-I and consequently in increased amounts of ‘free’ IGFs available for receptors in various maternal tissues as well as in the placenta [16].…”

Section: Introductionmentioning

confidence: 99%

Longitudinal Study of the Maternal Insulin-Like Growth Factor System before, during and after Pregnancy in Relation to Fetal and Infant Weight

et al. 2007

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Background: The maternal insulin-like growth factor (IGF) system is considered to be involved in fetal growth regulation. However, available data linking this system to fetal growth are contradictory and incomplete. Aims: To measure components of the IGF system before, during and after pregnancy in healthy women and to relate these results, and their changes during pregnancy, to fetal weight (gestational week 31) and birth weight. Methods: Serum concentrations of IGF-I, IGF-II, IGF-binding protein (IGFBP)-1, IGFBP-3 and IGFBP-3 protease activity were assessed in 23 women before conception, at weeks 8, 14, 20, 32 and 35 of pregnancy and 2 weeks postpartum. The data were analyzed using simple and multiple linear regression. Results: One third of the variability in fetal weight was explained by IGF-I in combination with IGFBP-3 protease activity, both assessed at gestational week 32 (p = 0.013). Birth weight was negatively correlated (r = –0.43 to –0.59) with IGFBP-1 at gestational week 20 (p = 0.041), 32 (p = 0.012) and 35 (p = 0.003). Conclusion: We propose there is a finely tuned balance among the components of the IGF system, providing a means for fetal growth regulation.

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Profiles of Insulin-Like Growth Factor Binding Proteins and the Protease Activity in the Maternal Circulation and Its Local Regulation Between Placenta and Decidua.

Abstract: Abstract.Insulin-like growth factors (IGF) and their specific binding proteins (IGFBPs) are believed to be important regulators of fetal growth.

Cited by 21 publications

References 35 publications

ADAM 12, a Disintegrin Metalloprotease, Interacts with Insulin-like Growth Factor-binding Protein-3

ADAM 12, a Disintegrin Metalloprotease, Interacts with Insulin-like Growth Factor-binding Protein-3

IGFs and IGF-binding proteins in the regulation of human ovarian and endometrial function

Longitudinal Study of the Maternal Insulin-Like Growth Factor System before, during and after Pregnancy in Relation to Fetal and Infant Weight

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