Profilin is a marker of severity in allergic respiratory diseases

Ruíz-Hornillos, Javier; López-Matas, M. Ángeles; Jimeno, Pilar Berges; Henríquez, Aythamy; Blanco, S.; Seoane‐Rodríguez, Marta; Mahíllo, Ignacio; Carnés, Jerónimo

doi:10.1111/all.14140

Cited by 15 publications

(9 citation statements)

References 31 publications

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“…This is congruent to other recent studies eg from Ruiz-Hornillos et al who found in a prospective case-control study that sensitization to profilin seems to be a marker of severity in patients with rhinoconjunctivitis and asthma mediated by pollen. 20 Previous observations also have suggested the importance of profilin as a marker of evolution and early marker of asthma severity which is in accordance to our results with a higher rate of self-reported asthma among patients sensitized to profilin. 16 , 21 , 22 …”

Section: Discussionsupporting

confidence: 93%

Clinical Relevance of Profilin Sensitization Concerning Oral Allergy Syndrome in Birch Pollen Sensitized Patients

Högerle

Nicoló

Gellrich

et al. 2022

JAA

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Purpose Many plant food allergens contain homologs to the major birch allergen Bet v 1; therefore, the majority of patients with birch pollen allergy suffer from oral allergy syndrome. We studied the relevance of sensitization of Bet v 1 and co-sensitization with profilin concerning the clinical manifestations of an oral allergy syndrome. Patients and Methods A total of 1119 patients with a positive skin prick test to birch and diagnostic for specific IgE to the allergen components Bet v 1 and profilin were analyzed retrospectively regarding complaints in the upper and lower airways, with demographic data as well as oral and gastrointestinal allergy symptoms. Results Patients with simultaneous specific IgE to the allergen components Bet v 1 and profilin have to deal more often with an oral allergy syndrome and intestinal reactions. They also suffer more frequently and severely from rhinitis symptoms and show a higher tendency for asthma. No increased number of systemic reactions was seen. Conclusion Patients with birch allergy recognizing both allergens have more probabilities of suffering from an oral allergy syndrome, and allergologists should be aware of this in order to give better advice to their patients.

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Section: Discussionsupporting

confidence: 93%

Clinical Relevance of Profilin Sensitization Concerning Oral Allergy Syndrome in Birch Pollen Sensitized Patients

Högerle

Nicoló

Gellrich

et al. 2022

JAA

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“…Profilins [20] and polcalcins are important respiratory panallergens. Despite their ubiquity in the plant kingdom, both panallergens are not recognized as major allergens and their clinical relevance remains poorly understood.…”

Section: Discussionmentioning

confidence: 99%

Ole e 3, a Candidate for in vivo Diagnosis of Polcalcin Sensitization

Moya¹,

González-Ruiz²,

Beitia³

et al. 2021

Int Arch Allergy Immunol

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Introduction: Polcalcins belong to the family of calcium-binding proteins. They are ubiquitous in the plant kingdom and highly conserved, which leads to these panallergens showing a high degree of inter-cross-reactivity. They are responsible for allergic polysensitization, and therefore, their diagnosis is necessary for correct selection of immunotherapy. The objectives were to develop a method to purify native polcalcin with intact allergenic properties and to validate its use for diagnosis of polcalcin sensitization. Methods: Ole e 3 was purified by immunoaffinity chromatography using anti-rChe a 3 polyclonal antibodies and identified by mass spectrometry. Calcium-binding assays were performed in immunoblot and ELISA assays. Diagnostic capacity of Ole e 3 was analyzed by ELISA and compared to ImmunoCAP with sera from a pollen-sensitized population. Cross-reactivity with other polcalcins was investigated by ImmunoCAP inhibition. Results: Immunogenicity of purified Ole e 3 was not affected by the addition of calcium. However, the presence of a calcium chelator agent completely inhibited IgG binding by immunoblot and produced a 32.3% reduction in IgE binding by ELISA. Ole e 3 enabled diagnosis of polcalcin-sensitized patients, and a good correlation was revealed with ImmunoCAP. A 50% inhibition in IgE binding was obtained with 2.8 ng of Ole e 3 for rBet v 4 and 3.9 ng for rPhl p 7. Discussion/Conclusion: Native Ole e 3 was purified by maintaining its allergenic properties. This innovative method enables obtaining this active native allergen to be used for in vivo diagnosis of polcalcin sensitization.

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“…1398 Further, recent studies showed that in certain geographic areas profilin sensitization represents a marker of more severe respiratory allergy in patients with pollen-mediated rhino-conjunctivitis and asthma, probably because in most cases it occurs in patients with multiple primary sensitizations to different sources. 1399 B) As a plant food allergen (Table 71)…”

Section: -Clinical Relevance Of Profilinmentioning

confidence: 99%

EAACIMolecular Allergology User's Guide 2.0

Dramburg

Hilger

Santos

et al. 2023

Pediatric Allergy Immunology

121

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Since the discovery of immunoglobulin E (IgE) as a mediator of allergic diseases in 1967, our knowledge about the immunological mechanisms of IgE‐mediated allergies has remarkably increased. In addition to understanding the immune response and clinical symptoms, allergy diagnosis and management depend strongly on the precise identification of the elicitors of the IgE‐mediated allergic reaction. In the past four decades, innovations in bioscience and technology have facilitated the identification and production of well‐defined, highly pure molecules for component‐resolved diagnosis (CRD), allowing a personalized diagnosis and management of the allergic disease for individual patients. The first edition of the “EAACI Molecular Allergology User's Guide” (MAUG) in 2016 rapidly became a key reference for clinicians, scientists, and interested readers with a background in allergology, immunology, biology, and medicine. Nevertheless, the field of molecular allergology is moving fast, and after 6 years, a new EAACI Taskforce was established to provide an updated document. The Molecular Allergology User's Guide 2.0 summarizes state‐of‐the‐art information on allergen molecules, their clinical relevance, and their application in diagnostic algorithms for clinical practice. It is designed for both, clinicians and scientists, guiding health care professionals through the overwhelming list of different allergen molecules available for testing. Further, it provides diagnostic algorithms on the clinical relevance of allergenic molecules and gives an overview of their biology, the basic mechanisms of test formats, and the application of tests to measure allergen exposure.

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Profilin is a marker of severity in allergic respiratory diseases

Cited by 15 publications

References 31 publications

Clinical Relevance of Profilin Sensitization Concerning Oral Allergy Syndrome in Birch Pollen Sensitized Patients

Clinical Relevance of Profilin Sensitization Concerning Oral Allergy Syndrome in Birch Pollen Sensitized Patients

Ole e 3, a Candidate for in vivo Diagnosis of Polcalcin Sensitization

EAACIMolecular Allergology User's Guide 2.0

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