Profiling bone morphogenetic proteins and transforming growth factor-βs by hTGF-β3 pre-treated coral-derived macroporous bioreactors: The power of one

Ripamonti, Ugo; Dix-Peek, Thérèse; Parak, Ruqayya; Milner, Brenda; Duarte, Raquel

doi:10.1016/j.biomaterials.2015.01.058

Cited by 22 publications

(92 citation statements)

References 27 publications

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“…The same group, has also shown that if the same coral material is loaded with hTGF-3 both the adjacent muscle and the macroporous bioreactor show upregulation of BMP-2 upregulation. 39 This finding correlates with the observation of bone formation occurring at the periphery of the graft but also could be the result of the recruiting of osteoprogenitor cells from the adjacent soft tissues.…”

Section: Animal Studiessupporting

confidence: 87%

“…32 Analyzing further the way that bone formation occurs within the corals it is of interest to mention the work of Ripamonti et al group. 38,39 A partially converted corral, composed of 7% hydroxyapatite and calcium carbonate was preloaded with verapamil (calcium channel blocker) or bipsosphonate zoledronate (osteoclast inhibitor) and implanted intramuscularly in baboons.…”

Section: Animal Studiesmentioning

confidence: 99%

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Is there a role of coral bone substitutes in bone repair?

Pountos

Giannoudis

2016

Injury

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Xenogeneic bone graft materials are an alternative to autologous bone grafting. Among such implants, coralline-derived bone grafts substitutes have a long track record as safe, biocompatible and osteoconductive graft materials. In this review, we present the available literature surrounding their use with special focus on the commercially available graft materials. Corals thanks to their chemical and structural characteristics similar to those of the human cancellous bone have shown great potential but clinical data presented to date is ambiguous with both positive and negative outcomes reported.Correct formulation and design of the graft to ensure adequate osteo-activity and resorption appears intrinsic to a successful outcome.

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Section: Animal Studiessupporting

confidence: 87%

Section: Animal Studiesmentioning

confidence: 99%

Is there a role of coral bone substitutes in bone repair?

Pountos

Giannoudis

2016

Injury

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“…Experimentation reporting the induction of bone formation by the hTGF-β 3 isoform has shown that the human recombinant protein is the most powerful inductive morphogen so far tested in primates (Figure 2; Ripamonti et al, 2008a,b, 2010, 2014, 2015). The Chacma baboon P. ursinus displayed unique results when compared to rodents, lagomorphs and canines.…”

Section: Redundancy and The Induction Of Bone Formaton By Multiple Homentioning

confidence: 99%

“…(Figure 2). Until recently, this question still remained unanswered and needed to be assigned (Klar et al, 2014; Ripamonti et al, 2014, 2015). …”

Section: Redundancy and The Induction Of Bone Formaton By Multiple Homentioning

confidence: 99%

“…Strikingly, the three mammalian isoforms, foremost the hTGF-β 3 isoform, are powerful inducers of endochondral bone formation when implanted in the rectus abdominis muscle of the Chacma baboon P. ursinus when combined with either insoluble collagenous bone matrices (Figure 2; Ripamonti et al, 2008a) or coral-derived macroporous bioreactors (Figure 3; Klar et al, 2014; Ripamonti et al, 2014, 2015). …”

Section: Redundancy and The Induction Of Bone Formaton By Multiple Homentioning

confidence: 99%

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Redundancy and Molecular Evolution: The Rapid Induction of Bone Formation by the Mammalian Transforming Growth Factor-β3 Isoform

Ripamonti

Duarte

Parak³

et al. 2016

Front. Physiol.

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The soluble osteogenic molecular signals of the transforming growth factor-β (TGF-β) supergene family are the molecular bases of the induction of bone formation and postnatal bone tissue morphogenesis with translation into clinical contexts. The mammalian TGF-β3 isoform, a pleiotropic member of the family, controls a vast array of biological processes including the induction of bone formation. Recombinant hTGF-β3 induces substantial bone formation when implanted with either collagenous bone matrices or coral-derived macroporous bioreactors in the rectus abdominis muscle of the non-human primate Papio ursinus. In marked contrast, the three mammalian TGF-βs do not initiate the induction of bone formation in rodents and lagomorphs. The induction of bone by hTGF-β3/preloaded bioreactors is orchestrated by inducing fibrin-fibronectin rings that structurally organize tissue patterning and morphogenesis within the macroporous spaces. Induced advancing extracellular matrix rings provide the structural anchorage for hyper chromatic cells, interpreted as differentiating osteoblasts re-programmed by hTGF-β3 from invading myoblastic and/or pericytic differentiated cells. Runx2 and Osteocalcin expression are significantly up-regulated correlating to multiple invading cells differentiating into the osteoblastic phenotype. Bioreactors pre-loaded with recombinant human Noggin (hNoggin), a BMPs antagonist, show down-regulation of BMP-2 and other profiled osteogenic proteins' genes resulting in minimal bone formation. Coral-derived macroporous constructs preloaded with binary applications of hTGF-β3 and hNoggin also show down-regulation of BMP-2 with the induction of limited bone formation. The induction of bone formation by hTGF-β3 is via the BMPs pathway and it is thus blocked by hNoggin. Our systematic studies in P. ursinus with translational hTGF-β3 in large cranio-mandibulo-facial defects in humans are now requesting the re-evaluation of “Bone: formation by autoinduction” in primate models including humans.

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Mechanistic insights into the spontaneous induction of bone formation

Ripamonti,

Duarte

2024

Biomaterials Advances

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Profiling bone morphogenetic proteins and transforming growth factor-βs by hTGF-β3 pre-treated coral-derived macroporous bioreactors: The power of one

Cited by 22 publications

References 27 publications

Is there a role of coral bone substitutes in bone repair?

Is there a role of coral bone substitutes in bone repair?

Redundancy and Molecular Evolution: The Rapid Induction of Bone Formation by the Mammalian Transforming Growth Factor-β3 Isoform

Mechanistic insights into the spontaneous induction of bone formation

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