Profiling candidate genes involved in wax biosynthesis in Arabidopsis thaliana by microarray analysis

Costaglioli, Patricia; Joubès, Jérôme; Garcia, Christel; Stef, Marianne; Arveiler, Benoı̂t; Lessire, René; Garbay, Bertrand

doi:10.1016/j.bbalip.2005.04.002

Cited by 72 publications

(60 citation statements)

References 65 publications

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“…Very surprising was the relatively high expression of 24 genes with a suggested function in lignin biosynthesis (Ehlting et al, 2005) and 13 genes implicated in suberin biosynthesis (CER3, CER6, and 11 LTP genes; Costaglioli et al, 2005) in T. caerulescens (Table IV). CER3 is known to be expressed in Arabidopsis roots, but the expression of CER6 in T. caerulescens roots is very different from the expression in Arabidopsis (Hannoufa et al, 1996;Hooker et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

Large Expression Differences in Genes for Iron and Zinc Homeostasis, Stress Response, and Lignin Biosynthesis Distinguish Roots of Arabidopsis thaliana and the Related Metal Hyperaccumulator Thlaspi caerulescens

et al. 2006

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The micronutrient zinc has an essential role in physiological and metabolic processes in plants as a cofactor or structural element in 300 catalytic and noncatalytic proteins, but it is very toxic when available in elevated amounts. Plants tightly regulate their internal zinc concentrations in a process called zinc homeostasis. The exceptional zinc hyperaccumulator species Thlaspi caerulescens can accumulate up to 3% of zinc, but also high amounts of nickel and cadmium, without any sign of toxicity. This should have drastic effects on the zinc homeostasis mechanism. We examined in detail the transcription profiles of roots of Arabidopsis thaliana and T. caerulescens plants grown under deficient, sufficient, and excess supply of zinc. A total of 608 zincresponsive genes with at least a 3-fold difference in expression level were detected in A. thaliana and 352 in T. caerulescens in response to changes in zinc supply. Only 14% of these genes were also zinc responsive in A. thaliana. When comparing A. thaliana with T. caerulescens at each zinc exposure, more than 2,200 genes were significantly differentially expressed ($5-fold and false discovery rate , 0.05). While a large fraction of these genes are of yet unknown function, many genes with a different expression between A. thaliana and T. caerulescens appear to function in metal homeostasis, in abiotic stress response, and in lignin biosynthesis. The high expression of lignin biosynthesis genes corresponds to the deposition of lignin in the endodermis, of which there are two layers in T. caerulescens roots and only one in A. thaliana.

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Section: Discussionmentioning

confidence: 99%

Large Expression Differences in Genes for Iron and Zinc Homeostasis, Stress Response, and Lignin Biosynthesis Distinguish Roots of Arabidopsis thaliana and the Related Metal Hyperaccumulator Thlaspi caerulescens

et al. 2006

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“…The FAR-produced aldehyde intermediates could be chain-shortened by a process distinct from α-oxidation. As an activity that could support a direct decarbonylative reaction, CER1 has been suggested to be an aldehyde decarbonylase in Arabidopsis, however, the assignment of this activity is far from clear [149,153]. Biochemical activities for aldehyde decarbonylation have been clearly observed in preparations from P. sativa leaves and the green algae Botryococcus braunii [154,155].…”

Section: α-Oxidation-while α-Oxidationmentioning

confidence: 98%

Biosynthesis and function of polyacetylenes and allied natural products

Minto

Blacklock

2008

Progress in Lipid Research

296

228

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Polyacetylenic natural products are a substantial class of often unstable compounds containing a unique carbon-carbon triple bond functionality, that are intriguing for their wide variety of biochemical and ecological functions, economic potential, and surprising mode of biosynthesis. Isotopic tracer experiments between 1960 and 1990 demonstrated that the majority of these compounds are derived from fatty acid and polyketide precursors. During the past decade, research into the metabolism of polyacetylenes has swiftly advanced, driven by the cloning of the first genes responsible for polyacetylene biosynthesis in plants, moss, fungi, and actinomycetes, and the initial characterization of the gene products.The current state of knowledge of the biochemistry and molecular genetics of polyacetylenic secondary metabolic pathways will be presented together with an up-to-date survey of new terrestrial and marine natural products, their known biological activities, and a discussion of their likely metabolic origins.

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“…Two genes encoding condensing enzymes of these complexes are present in our library, a KCS and a b-ketoacyl CoA reductase (KCR). These two genes show, respectively, high similarity to At5g43760 (91% similarity) and At1g67730 (79% similarity), which have been identified as possible candidates for cuticular wax biosynthesis (Costaglioli et al, 2005). Mutations in KCS (KCS1 [Todd et al, 1999]; FIDDLEHEAD [Yephremov et al, 1999;Pruitt et al, 2000]; CUT1 [Millar et al, 1999]; CER6 [Fiebig et al, 2000]) and KCR (Dietrich et al, 2005) have a pronounced effect on cuticular wax deposition.…”

Section: Synthesis Of Aliphatic Monomersmentioning

confidence: 99%

A Genomic Approach to Suberin Biosynthesis and Cork Differentiation

Soler

Serra²,

Molinas³

et al. 2007

Plant Physiology

143

139

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Cork (phellem) is a multilayered dead tissue protecting plant mature stems and roots and plant healing tissues from water loss and injuries. Cork cells are made impervious by the deposition of suberin onto cell walls. Although suberin deposition and cork formation are essential for survival of land plants, molecular studies have rarely been conducted on this tissue. Here, we address this question by combining suppression subtractive hybridization together with cDNA microarrays, using as a model the external bark of the cork tree (Quercus suber), from which bottle cork is obtained. A suppression subtractive hybridization library from cork tree bark was prepared containing 236 independent sequences; 69% showed significant homology to database sequences and they corresponded to 135 unique genes. Out of these genes, 43.5% were classified as the main pathways needed for cork biosynthesis. Furthermore, 19% could be related to regulatory functions. To identify genes more specifically required for suberin biosynthesis, cork expressed sequence tags were printed on a microarray and subsequently used to compare cork (phellem) to a non-suberin-producing tissue such as wood (xylem). Based on the results, a list of candidate genes relevant for cork was obtained. This list includes genes for the synthesis, transport, and polymerization of suberin monomers such as components of the fatty acid elongase complexes, ATP-binding cassette transporters, and acyltransferases, among others. Moreover, a number of regulatory genes induced in cork have been identified, including MYB, No-Apical-Meristem, and WRKY transcription factors with putative functions in meristem identity and cork differentiation.

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Profiling candidate genes involved in wax biosynthesis in Arabidopsis thaliana by microarray analysis

Cited by 72 publications

References 65 publications

Large Expression Differences in Genes for Iron and Zinc Homeostasis, Stress Response, and Lignin Biosynthesis Distinguish Roots of Arabidopsis thaliana and the Related Metal Hyperaccumulator Thlaspi caerulescens

Large Expression Differences in Genes for Iron and Zinc Homeostasis, Stress Response, and Lignin Biosynthesis Distinguish Roots of Arabidopsis thaliana and the Related Metal Hyperaccumulator Thlaspi caerulescens

Biosynthesis and function of polyacetylenes and allied natural products

A Genomic Approach to Suberin Biosynthesis and Cork Differentiation

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