Profiling dendritic cell subsets in the patients with active pulmonary tuberculosis

Lu, Yuan; Xiao, De Qian; Liang, Kui Di; Zhang, Jun Ai; Wang, Wan Dang; Yu, Shi Yan; Zheng, Bi Ying; Gao, Yu; Dai, You Chao; Jia, Yan; Chen, Chen; Zhuang, Ze Gang; Wang, Xin; Fu, Xiao; Zhou, Yong; Zhong, Jixin; Chen, Zheng W.; Xu, Jun

doi:10.1016/j.molimm.2017.08.007

Cited by 13 publications

(10 citation statements)

References 39 publications

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“…Together, these results project pDCs as a key immune cell in the lungs of PTB macaques where they are primed for rapid ll Article type I IFN production, prolonged survival, and may contribute to suppression and exhaustion of T cell responses. Finally, circulating pDCs have been reportedly depleted in PTB patients when compared with healthy controls (Lu et al, 2017). These results are consistent with our data from humans and macaques where peripheral pDCs are depleted specifically during PTB, suggesting that pDCs are actively recruited to the Mtb-inflamed lung from the circulation to propagate the type I IFN signaling cascade.…”

Section: Discussionsupporting

confidence: 91%

The immune landscape in tuberculosis reveals populations linked to disease and latency

Esaulova

Das

Singh

et al. 2021

Cell Host & Microbe

138

126

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Section: Discussionsupporting

confidence: 91%

The immune landscape in tuberculosis reveals populations linked to disease and latency

Esaulova

Das

Singh

et al. 2021

Cell Host & Microbe

138

126

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“…Mustafa et al found that the level of soluble FasL was significantly higher in children than that in adults, and FasL may function in immune modulation and pathogenesis of TB [ 29 ]. Lu et al found that CD83 and CCR7 are highly expressed in myeloid dendritic cell (DC) from active pulmonary tuberculosis patients, but low expressed in plasmacytoid DC [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

Identification of Potential Biomarkers and Related Transcription Factors in Peripheral Blood of Tuberculosis Patients

Xie

Chao

Teng

et al. 2020

IJERPH

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Tuberculosis (TB), one major threat to humans, can infect one third of the worldwide population, and cause more than one million deaths each year. This study aimed to identify the effective diagnosis and therapy biomarkers of TB. Hence, we analyzed two microarray datasets (GSE54992 and GSE62525) derived from the Gene Expression Omnibus (GEO) database to find the differentially expressed genes (DEGs) of peripheral blood mononuclear cell (PBMC) between TB patients and healthy specimens. Functional and pathway enrichment of the DEGs were analyzed by Metascape database. Protein-protein interaction (PPI) network among the DEGs were constructed by STRING databases and visualized in Cytoscape software. The related transcription factors regulatory network of the DEGs was also constructed. A total of 190 DEGs including 36 up-regulated genes and 154 down-regulated genes were obtained in TB samples. Gene functional enrichment analysis showed that these DEGs were enriched in T cell activation, chemotaxis, leukocyte activation involved in immune response, cytokine secretion, head development, etc. The top six hub genes (namely, LRRK2, FYN, GART, CCR7, CXCR5, and FASLG) and two significant modules were got from PPI network of DEGs. Vital transcriptional factors, such as FoxC1 and GATA2, were discovered with close interaction with these six hub DEGs. By systemic bioinformatic analysis, many DEGs associated with TB were screened, and these identified hub DEGs may be potential biomarkers for diagnosis and treatment of TB in the future.

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“…The expression of BTLA, DC maturation markers CD83 and CCR7, DC antigen presenting molecules HLA-DR, and costimulatory molecules CD80 and CD86 on tDCs and DC subsets was determined by flow cytometry (15,26,27). Heparin sodiumanticoagulated whole blood (100 µL) was probed with antibodies for 30 min, followed by lysis of red blood cells.…”

Section: Flow Cytometry Analysismentioning

confidence: 99%

“…It is suggested that DCs play a pivotal role in immune responses to TB (14). In fact, we recently demonstrated that the absolute number of total DCs (tDCs), mDCs, and pDCs in individuals with active pulmonary tuberculosis (APT) was decreased compared with healthy controls (HCs); this decrease was associated with prolonged and complicated TB, TB burden, and over-reactive inflammation (15). There was also an altered expression of the maturation surface markers (CD83 and CCR7), the antigen presenting molecule HLA-DR, and the co-stimulatory molecules (CD80 and CD86) of DCs in APT patients (15).…”

Section: Introductionmentioning

confidence: 99%

“…In fact, we recently demonstrated that the absolute number of total DCs (tDCs), mDCs, and pDCs in individuals with active pulmonary tuberculosis (APT) was decreased compared with healthy controls (HCs); this decrease was associated with prolonged and complicated TB, TB burden, and over-reactive inflammation (15). There was also an altered expression of the maturation surface markers (CD83 and CCR7), the antigen presenting molecule HLA-DR, and the co-stimulatory molecules (CD80 and CD86) of DCs in APT patients (15). These data suggest that Mtb might modulate the DC functions during induction or development of TB, but further studies are needed to define the TBinduced changes in DC function and to understand the underlying mechanism.…”

Section: Introductionmentioning

confidence: 99%

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BTLA-Expressing Dendritic Cells in Patients With Tuberculosis Exhibit Reduced Production of IL-12/IFN-α and Increased Production of IL-4 and TGF-β, Favoring Th2 and Foxp3+ Treg Polarization

Zhang

Wang

et al. 2020

Front. Immunol.

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Little is known about how tuberculosis (TB) impairs dendritic cell (DC) function and anti-TB immune responses. We previously showed that the B and T lymphocyte attenuator (BTLA), an immune inhibitory receptor, is involved in TB pathogenesis. Here, we examined whether BTLA expression in TB affects phenotypic and functional aspects of DCs. Active TB patients exhibited higher expression of BTLA in myeloid dendritic cells (mDCs) and plasmacytoid DCs (pDCs) subsets compared with healthy controls (HCs). BTLA expression was similarly high in untreated TB, TB relapse, and sputum-bacillus positive TB, but anti-TB therapy reduced TB-driven increases in frequencies of BTLA + DCs. BTLA + DCs in active TB showed decreased expression of the DC maturation marker CD83, with an increased expression of CCR7 in mDCs. BTLA + DCs in active TB displayed a decreased ability to express HLA-DR and to uptake foreign antigen, with a reduced expression of the co-stimulatory molecule CD80, but not CD86. Functionally, BTLA + DCs in active TB showed a decreased production of IL-12 and IFN-α as well as a reduced ability to stimulate allogeneic T-cell proliferative responses. BTLA + mDCs produced larger amounts of IL-4 and TGF-β than BTLA − mDCs in both HCs and APT patients. BTLA + DCs from active TB patients showed a reduced ability to stimulate Mtb antigen-driven Th17 and Th22 polarizations as compared to those from HCs. Conversely, these BTLA + DCs more readily promoted the differentiation of T regulatory cells (Treg) and Th2 than those from HCs. These findings suggest that TB-driven BTLA expression in DCs impairs the expression of functional DC surrogate markers and suppress the ability of DCs to induce anti-TB Th17 and Th22 response while promoting Th2 and Foxp3 + Tregs.

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Profiling dendritic cell subsets in the patients with active pulmonary tuberculosis

Cited by 13 publications

References 39 publications

The immune landscape in tuberculosis reveals populations linked to disease and latency

The immune landscape in tuberculosis reveals populations linked to disease and latency

Identification of Potential Biomarkers and Related Transcription Factors in Peripheral Blood of Tuberculosis Patients

BTLA-Expressing Dendritic Cells in Patients With Tuberculosis Exhibit Reduced Production of IL-12/IFN-α and Increased Production of IL-4 and TGF-β, Favoring Th2 and Foxp3+ Treg Polarization

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