Profiling fungal cultures in situ via the droplet-LMJ-SSP coupled with UPLC-PDA-HRMS-MS/MS

Sica, VP; Raja, HA; El‐Elimat, Tamam; Kertész, Vilmos; Berkel, GJ Van; Pearce, CJ; Oberlies, NH

doi:10.1055/s-0035-1556370

Cited by 1 publication

(1 citation statement)

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“…The key to these techniques is that little-to-no sample preparation is needed before analysis. To date, in vivo mass spectrometric characterization has been applied to chemically characterize foods, biological fluids, plants, fungi, and some tissues (e.g., skin). , In these types of analyses, minimal damage or perturbation to the physiological functions of the living system can be maintained. However, the impact of such MS characterization methods remains detrimental for smaller systems like biology-on-a-chip devices, which are more susceptible to perturbations.…”

Section: Introductionmentioning

confidence: 99%

In Situ Chemical Monitoring and Imaging of Contents within Microfluidic Devices Having a Porous Membrane Wall Using Liquid Microjunction Surface Sampling Probe Mass Spectrometry

Cahill

Khalid

Retterer

et al. 2020

J. Am. Soc. Mass Spectrom.

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The ability to observe dynamic chemical processes (e.g., signaling, transport, etc.) in vivo or in situ using nondestructive chemical imaging opens a new door to understanding the complex dynamics of developing biological systems. With the advent of “biology-on-a-chip” devices has come the ability to monitor dynamic chemical processes in a controlled environment, using these engineered habitats to capture key features of natural systems while allowing visual observation of system development. Having the capability to spatially and temporally map the chemical signals within these devices may yield new insights into the forces that drive biosystem development. Here, a porous membrane sealed microfluidic device was designed to allow normal microfluidic operation while enabling continuous, location specific sampling and chemical characterization by liquid microjunction surface sampling probe mass spectrometry (LMJ-SSP MS). LMJ-SSP was used to extract fluids with nL-to-μL/min flow rates directly from selected areas of the microfluidic device without negatively impacting the device function. These extracts were subsequently characterized using MS. This technique was used to acquire MS images of the entirety of several multi-input microfluidic devices having different degrees of fluid mixing. LMJ-SSP MS imaging visualized the spatial distribution of chemical components within the microfluidic channels and could visualize chemical reactions occurring in the device. These microfluidic devices with a porous membrane wall are wholly compatible with the construction of biology-on-a-chip devices. This ultimately would enable correlation of biosystem physical structure with an evolving chemical environment

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