Profiling Humoral Immunity After Mixing and Matching COVID-19 Vaccines Using SARS-CoV-2 Variant Protein Microarrays

Kuo, Ho‐Chang; Kuo, Kuang-Che; Du, Pin-Xian; Keskin, Batuhan Birol; Su, Wenyu; Ho, Tzong Shiann; Tsai, Pei‐Shan; Pau, Chi Ho; Shih, Hsi-Chang; Huang, Ying‐Hsien; Weng, Ken-Pen; Syu, Guan‐Da

doi:10.1016/j.mcpro.2023.100507

Cited by 6 publications

(4 citation statements)

References 43 publications

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“…RBD and ECD specific antibody binding profiles were different, which were observed in our previous and other studies. 35–38 As BNT162b2 was developed targeting the RBD region, more robust neutralization was observed within SARS-CoV-2 RBDs, and antibodies bound to full-length spike proteins might cause steric hindrance, preventing distinctive binding. 39…”

Section: Discussionmentioning

confidence: 99%

Profiling humoral responses to COVID-19 immunization in Kawasaki disease using SARS-CoV-2 variant protein microarrays

Keskin¹,

Li²,

Du³

et al. 2023

Analyst

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Section: Discussionmentioning

confidence: 99%

Profiling humoral responses to COVID-19 immunization in Kawasaki disease using SARS-CoV-2 variant protein microarrays

Keskin¹,

Li²,

Du³

et al. 2023

Analyst

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“…The pre-made CoVariant protein arrays were removed from the −80°C refrigerator and defrosted at room temperature. The detailed assay procedures were described previously [ 8 ]. Briefly, arrays were blocked, incubated with 50 μl of 50× diluted serum samples in tris-buffered saline with 0.1% tween 20 (TBST) with 0.1% BSA for 1 h, washed three times, incubated with Cy3-labelled anti-human IgG/IgA/IgM and Cy5-labelled ACE2 cocktails for 1 h, washed three times, and dried.…”

Section: Methodsmentioning

confidence: 99%

“…The raw signal intensity of each spot was extracted by GenePix Pro software and analysed and exported as the foreground minus background. The surrogate neutralizing activities were measured using the Cy5 fluorescence: 1 − (ACE2 signals with serum/ACE2 signals without serum) × 100% [ 7 , 8 ]. For binding antibodies, the signals of the Cy3-labelled anti-human were adjusted according to anti-His signals to normalize the amount of protein and presented as the relative fluorescence intensity (RFI).…”

Section: Methodsmentioning

confidence: 99%

Rituximab, but not other biologics, impairs humoral immunity in patients with rheumatoid arthritis—a study using CoVariant protein arrays

Lin,

Du,

Tsai

et al. 2023

Rheumatology Advances in Practice

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Objectives Rheumatoid arthritis (RA) is an autoimmune disease characterized by chronic inflammation and joint destruction. Biologics are crucial to achieving treat-to-target goals in patients with RA. The global spread and continuous variation of SARS-CoV-2 necessitate monitoring variant-specific humoral responses post-vaccination. This study aims to investigate how different biologic treatments for vaccinated RA patients may affect their neutralizing antibodies against multiple SARS-CoV-2 variants. Methods We recruited RA patients who had received three doses of conventional SARS-CoV-2 vaccines and were treated with various biologics, e.g., TNF inhibitor (etanercept), IL-6 inhibitor (tocilizumab), CTLA4-Ig (abatacept) or anti-CD20 (rituximab). Serum samples were used to profile the binding and neutralizing antibodies using our own SARS-CoV-2 variant (CoVariant) protein array, developed previously. Results Compared to healthy controls, only RA therapy with rituximab showed a reduction in neutralizing antibodies capable of targeting spike proteins in SARS-CoV-2 wild-type and most variants. This reduction is not observed in binding antibodies against SARS-CoV-2 wild-type or its variants. Conclusions After receiving three doses of SARS-CoV-2 vaccination, RA patients who underwent Rituximab treatment generated sufficient antibodies but exhibited lower neutralizing activities against wild-type and multiple variants, including current Omicron. Other biological DMARDs, e.g., TNF inhibitor, IL-6 inhibitor, and CTLA4-Ig, did not show obvious inhibition. Lay summary What does this mean for patients ? This research examined the impact of various biologic treatments (a type of drug) on the immune response of rheumatoid arthritis (RA) patients to COVID-19 vaccination. The study revealed that RA patients treated with rituximab had significantly lower levels of neutralizing antibodies against various SARS-CoV-2 variants, including Omicron, even after receiving three vaccine doses. Neutralizing antibodies defend the body from organisms that cause disease, such as viruses, by preventing them from entering our cells. In contrast, RA patients receiving other biologic therapies, including TNF inhibitors, IL-6 inhibitors, or CTLA4-Ig, did not experience the same reduction in neutralizing antibodies. No significant difference was found in serum antibodies, regardless of the specific biologic therapy (TNF inhibitors, IL-6 inhibitors, CTLA4-Ig, or anti-CD20). This study highlights the different immune responses to COVID-19 vaccination in patients with RA treated with various biologics. Therefore, individuals with RA who are considering or are currently undergoing biologic treatment should engage in thorough discussions with their healthcare providers regarding vaccination strategies. In summary, this study underscores the necessity for personalized vaccination strategies for RA patients based on their specific treatment regimens to ensure robust protection against evolving COVID-19 variants.

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“…Sistem Pendukung Keputusan dapat dijelaskan sebagai proses pemilihan alternatif terbaik dari beberapa alternatif secara sistematis untuk digunakan sebagai suatu cara pemecahan masalah dengan memanfaat-kan teknologi atau sistem tertentu. Pada penelitian sistem pendukung keputusan dijabarkan sebagai sistem informasi interaktif yang menyediakan informasi, pemodelan, dan pemanipulasian data yang membantu dalam pengambilan keputusan [7], [8].…”

Section: Pendahuluanunclassified

Perhitungan Metode Profile Matching Dalam Seleksi Rumah Singgah Pada Komunitas Akhwat Sholehah (Koas)

Maharani,

Anggraeni,

Nurwati

2024

JOCSTEC

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Metode Profile Matching merupakan salah satu dari Sistem Pendukung Keputusan, yaitu suatu system berbasis komputer guna membantu para pengambil keputusan dalam mengatasi permasalahannya untuk mendapatkan atau menghasilkan keputusan yang tepat. [spk] perhitungan dari metode profile matching cukup sederhana, yaitu dengan membandingkan GAP dengan nilai alternative dan kriteria. Ada beberapa hal yang penting yang perlu diketahui mengenai analisis GAP, yaitu table nilai bobot GAP [1]. Selain itu, analisis GAP juga harus memiliki konsep skala prioritas untuk stiap kriteria Karena kemudahan perhitungan dari metode profile matching serta kebutuhan dan tujuan penelitian yang juga sederhana maka peneliti mengambil judul implementasi perhitungan metode profile matching untuk membantu peneliti memberikan jawaban pada komutias akhwat sholeha khususnya kisaran dalam mendapatkan rumah singgah dakwah yang tepat atau cocok untuk dihuni. Adapun tahapan perhitungan dari Profile Matching adalah Penentuan Bobot Nilai GAP, lalu perhitungan dan pengelompokkan core factor dan secoundary factor, selanjutnya perhitungan nilai total tiap kompetensi kriteria dan terakhir perhitungan rangking [2], [3], [4]. Setelah mendapatkan hasil perhitungan yang tepat terhadap rumah singgah dakwah, perhitungan dihentikan dan hasil seleksi diberikan kepada founder KoAS. Hasil dari seleksi rumah singgah dakwah pada Komunitas akhwat Sholehah (KoAS) Kisaran berupa nama dan nilai.

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Profiling Humoral Immunity After Mixing and Matching COVID-19 Vaccines Using SARS-CoV-2 Variant Protein Microarrays

Cited by 6 publications

References 43 publications

Profiling humoral responses to COVID-19 immunization in Kawasaki disease using SARS-CoV-2 variant protein microarrays

Profiling humoral responses to COVID-19 immunization in Kawasaki disease using SARS-CoV-2 variant protein microarrays

Rituximab, but not other biologics, impairs humoral immunity in patients with rheumatoid arthritis—a study using CoVariant protein arrays

Perhitungan Metode Profile Matching Dalam Seleksi Rumah Singgah Pada Komunitas Akhwat Sholehah (Koas)

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