Profiling of a few immune responsive genes expressed in postlarvae of Fenneropenaeus indicus challenged with Vibrio harveyi D3

Nayak, Sagar; Ajay, K.M.; Ramaiah, N.; Meena, Ramavatar; Sreepada, R.A.

doi:10.1016/j.jip.2011.04.001

Cited by 14 publications

(11 citation statements)

References 19 publications

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“…Four of the five categories with the highest fold enrichment include genes primarily involved in production of energy in the mitochondria, indicating an increased energy demand to mount an effective immune response (Nayak et al, 2011) or general stress response. Herein, genes involved in the production of reactive oxygen species (ROS) (NADH-ubiquinone oxidoreductase chain 1 and cytochrome c oxidase subunits) were among enriched processes in sea fans exposed to the Aplanochytrium .…”

Section: Discussionmentioning

confidence: 99%

Immune response of the Caribbean sea fan, Gorgonia ventalina, exposed to an Aplanochytrium parasite as revealed by transcriptome sequencing

Burge¹,

Mouchka²,

Harvell³

et al. 2013

Front. Physiol.

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Coral reef communities are undergoing marked declines due to a variety of stressors including disease. The sea fan coral, Gorgonia ventalina, is a tractable study system to investigate mechanisms of immunity to a naturally occurring pathogen. Functional studies in Gorgonia ventalina immunity indicate that several key pathways and cellular components are involved in response to natural microbial invaders, although to date the functional and regulatory pathways remain largely un-described. This study used short-read sequencing (Illumina GAIIx) to identify genes involved in the response of G. ventalina to a naturally occurring Aplanochytrium spp. parasite. De novo assembly of the G. ventalina transcriptome yielded 90,230 contigs of which 40,142 were annotated. RNA-Seq analysis revealed 210 differentially expressed genes in sea fans exposed to the Aplanochytrium parasite. Differentially expressed genes involved in immunity include pattern recognition molecules, anti-microbial peptides, and genes involved in wound repair and reactive oxygen species formation. Gene enrichment analysis indicated eight biological processes were enriched representing 36 genes, largely involved with protein translation and energy production. This is the first report using high-throughput sequencing to characterize the host response of a coral to a natural pathogen. Furthermore, we have generated the first transcriptome for a soft (octocoral or non-scleractinian) coral species. Expression analysis revealed genes important in invertebrate innate immune pathways, as well as those whose role is previously un-described in cnidarians. This resource will be valuable in characterizing G. ventalina immune response to infection and co-infection of pathogens in the context of environmental change.

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Section: Discussionmentioning

confidence: 99%

Immune response of the Caribbean sea fan, Gorgonia ventalina, exposed to an Aplanochytrium parasite as revealed by transcriptome sequencing

Burge¹,

Mouchka²,

Harvell³

et al. 2013

Front. Physiol.

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“…Higher levels of ferritin have already been shown on our previous study on F. indicus (Nayak et al . ). From this it can be hypothesized that higher expression levels of the ferritin gene signify its defence against invading bacteria, as shown earlier by Marcelis ().…”

Section: Discussionmentioning

confidence: 97%

“…Lacking immunological memory, the invertebrate immune system is dependent on specific innate molecules as a defence against external pathogens (Lee & Soderhall 2000). In a previous study (Nayak et al 2011), it was observed that the two most important immune-related genes, viz. translationally controlled tumour protein (TCTP) and ferritin, were substantially upregulated in F. indicus post-larvae (PL) following bath challenge with V. harveyi D3.…”

Section: Introductionmentioning

confidence: 96%

“…; Nayak et al . ). In view of the general lack of molecular information on immune‐related genes from this commercially valuable shrimp species, full‐length cloning [using rapid amplification of complementary DNA ends (RACE)] of both TCTP and ferritin genes, and sequencing, were carried out to investigate their possible functional role(s) and their phylogenetic relatedness/diversity.…”

Section: Introductionmentioning

confidence: 97%

“…In a previous study (Nayak et al . ), it was observed that the two most important immune‐related genes, viz. translationally controlled tumour protein ( TCTP ) and ferritin, were substantially upregulated in F. indicus post‐larvae (PL) following bath challenge with V. harveyi D3.…”

Section: Introductionmentioning

confidence: 99%

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Full‐length cloning and phylogenetic analyses of translationally controlled tumour protein and ferritin genes from the Indian white prawn, Fenneropenaeus indicus (H. Milne Edwards)

Nayak

Ramaiah

Meena

et al. 2013

Journal of Fish Diseases

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Elucidation, through molecular analyses, of bacterial afflictions in commercially important aquaculture-reared shrimps is pivotal for the prevention and/or control of disease outbreaks. In this study, we examined the phylogenetic relatedness and compared the possible immune-related functional roles of both translationally controlled tumour protein (TCTP) and ferritin genes with previous studies. Both TCTP and ferritin genes were substantially upregulated in the Indian white prawn, Fenneropenaeus indicus (H. Milne Edwards), post-larvae following bath challenge with the virulent strain of bacteria, Vibrio harveyi D3. Full-length cloning of these genes by rapid amplification of complementary DNA ends -polymerase chain reaction (RACE-PCR) yielded 727-base pair (bp)-long TCTP and 1212-bp-long ferritin gene sequences. Their open reading frames (ORFs) were 507 and 510 bp, respectively. The TCTP-ORF coded for 168 amino acids with three substitutions at positions 37, 141, 155, and the ferritin ORF coded for 170 amino acids with no species-specific substitutions. Phylogenetic analysis suggested the closest relatedness of both TCTP and ferritin from F. indicus to Chinese white prawn, Fenneropenaeus chinensis (Osbeck). In addition to reporting the full-length sequences of these immune-relevant genes, this study highlighted their conserved natures, which perhaps make them important defence-related proteins in the innate immune system of F. indicus.

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Ontogenetic and expression of different genes involved in the Toll pathway of black tiger shrimp (Penaeus monodon) following immersion challenge with Vibrio harveyi and white spot syndrome virus (WSSV)

et al. 2018

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Profiling of a few immune responsive genes expressed in postlarvae of Fenneropenaeus indicus challenged with Vibrio harveyi D3

Cited by 14 publications

References 19 publications

Immune response of the Caribbean sea fan, Gorgonia ventalina, exposed to an Aplanochytrium parasite as revealed by transcriptome sequencing

Immune response of the Caribbean sea fan, Gorgonia ventalina, exposed to an Aplanochytrium parasite as revealed by transcriptome sequencing

Full‐length cloning and phylogenetic analyses of translationally controlled tumour protein and ferritin genes from the Indian white prawn, Fenneropenaeus indicus (H. Milne Edwards)

Ontogenetic and expression of different genes involved in the Toll pathway of black tiger shrimp (Penaeus monodon) following immersion challenge with Vibrio harveyi and white spot syndrome virus (WSSV)

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