Profiling of chicken adipose tissue gene expression by genome array

Wang, Hongbao; Li, Hui; Wang, Qigui; Zhang, Xin Yu; Wang, Shouzhi; Wang, Yuxiang; Wang, Xiuping

doi:10.1186/1471-2164-8-193

Cited by 84 publications

(86 citation statements)

References 32 publications

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“…In the present study, despite exhibiting similar trends in expression level over the embryonic period (from E7 to E21), significant differences in MSTN mRNA expression level were observed between the Fat and Lean lines, particularly on E13, when levels were higher in the Fat line. These differences may be attributed to the divergent selection for abdominal fat percentage, plasma VLDL concentration and body weight at 7 weeks of age (Wang et al, 2007a;Wang et al, 2009).…”

Section: Discussionmentioning

confidence: 96%

“…These lines have been selected divergently using abdominal fat percentage, plasma very low-density lipoprotein (VLDL) concentration and body weight as selection criteria, since 1996 (Wang et al, 2007a;Wang et al, 2009). The percentages of abdominal fat in the two lines have diverged extensively after twelve generations, reaching 4.28% in the Fat line and 1.22% in the Lean line at 7 weeks of age, with no difference between lines in body weight (Wang et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

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Changes to daily feed intake during the laying period alters embryonicMSTNandMYOGgene expression in genetically fat and lean lines of chickens

Shan

et al. 2013

British Poultry Science

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1. Broiler breeders are unable to self-regulate feed intake to a level that permits reproductive function and restricted feeding is normal for broiler parent stock. The aim of this study was to determine whether myostatin (MSTN) and myogenin (MYOG) mRNA expression could be altered in relation to differences in body composition of the embryos and the hen's diet. 2. A 2 × 2 factorial experiment was conducted with two dietary intake levels (100% and 75% of nutrition recommendation) and two broiler genotypes (Fat line and Lean line). Hens (384 of each line) were randomly divided at 23 weeks of age into 4 treatments, with each treatment represented by 12 replicates of 16 birds each. The experiment started when the rate of lay reached 5% and continued until 54 weeks of age. 3. There were significant effects of line and dietary intake, and interactions between them on MSTN and MYOG mRNA expression levels in embryonic tissues. The trend in MSTN mRNA expression level over the embryonic period was similar between lines. Low daily feed intake did not alter the trend in MSTN mRNA expression levels in either line, but significantly changed the peak values. MYOG mRNA expression varied by line and over time, with a delay in the Lean line. Low daily feed intake influenced the trend in MYOG mRNA expression levels in both lines and delayed its onset.

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Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Changes to daily feed intake during the laying period alters embryonicMSTNandMYOGgene expression in genetically fat and lean lines of chickens

Shan

et al. 2013

British Poultry Science

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“…The Northeast Agricultural University (NEAU) broiler lines divergently selected for abdominal fat content (NEAUHLF) in lean and fat chicken lines (Wang et al, 2007a) derived from a commercial Arbor Acres grandsire line were created at the NEAU Animal Breeding Center in 1996. The two lines chicken has been selected divergently using percentage of abdominal fat weight (%AFW) and plasma very low-density lipoprotein (VLDL) concentration as selection criteria.…”

Section: Lean and Fat Lines Of Chickensmentioning

confidence: 99%

Microarray analysis of genes differentially expressed in the liver of lean and fat chickens

Wang

Zhang

et al. 2010

Animal

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Excessive accumulation of lipids in the adipose tissue is one of the main problems faced by the broiler industry nowadays. In chicken, lipogenesis occurs essentially in the liver, in which much of the triglycerides that accumulate in avian adipose tissue are synthesized. In order to better understand the gene expression and its regulation in chicken liver, the gene expression profiles of liver at developmental stages of chicken (1 week, 4 weeks and 7 weeks of age) were investigated and differentially expressed genes between lean and fat chicken lines divergently selected for abdominal fat content for eight generations were screened. Our data indicated that 4 weeks of age was a very important stage on chicken liver lipogenesis compared to 1 week and 7 weeks of age, and the glycometabolism in chicken liver could be related to lipid metabolism and the difference of glycometabolism could be another potential reason for the fat and lean phenotype occurrence besides the difference of lipogenesis in chicken liver. Our result have established groundwork for further study of the basic genetic control of chicken obesity and will benefit chicken research communities as well as researches that use chicken as a model organism for developmental biology and human therapeutics.

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“…Even though many global surveys have been performed for the visceral tissues (Wang et al, 2007), this study is the first to examine gene expression profiles in breast tissue using 3 individual pigments in broiler diets. The data obtained here indicate that the differentially expressed genes are associated with broiler muscles (Tables 2 and 3), although some important genes involved in the regulatory mechanism of pigments were common among the treatment groups, and many other genes, most in fact, were not differentially expressed between the groups.…”

Section: Discussionmentioning

confidence: 99%

Identification of genes involved in regulatory mechanism of pigments in broiler chickens

Tarique¹,

Yang²,

Mohsina³

et al. 2014

Genet. Mol. Res.

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ABSTRACT.Chicken is an important model organism that unites the evolutionary gap between mammals and other vertebrates and provide major source of protein from meat and eggs for all over the world population. However, specific genes underlying the regulatory mechanism of broiler pigmentation have not yet been determined. In order to better understand the genes involved in the mechanism of pigmentation in the muscle tissues of broilers, the Affymetrix microarray hybridization experiment platform was used to identify gene expression profiles at 7 weeks of age. Broilers fed canthaxanthin, natural lutein, and orangeII pigments (100 mg/kg) were used to explore gene expression profiles). Our data showed that the 7th week of age was a very important phase with regard to gene expression profiles. We identified a number of differentially expressed genes; in canthaxanthin, natural lutein, and orangeII, there were 54 (32 upregulated and 22 downregulated), 23 (15 upregulated and 8 downregulated), and 7 (5 upregulated and 2 downregulated) known genes, respectively. Our data indicate that the numbers of differentially expressed genes were more upregulated than downregulated, and several genes showed conserved signaling to previously known functions. Thus, functional characterization of differentially expressed genes revealed several categories that are involved in important biological processes, including pigmentation, growth, molecular mechanisms, fat metabolism, cell proliferation, immune response, lipid metabolism, and protein synthesis and degradation. The results of the present study demonstrate that the genes associated with canthaxanthin, natural lutein, and orangeII are key regulatory genes that control the regulatory mechanisms of pigmentation.

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Profiling of chicken adipose tissue gene expression by genome array

Cited by 84 publications

References 32 publications

Changes to daily feed intake during the laying period alters embryonicMSTNandMYOGgene expression in genetically fat and lean lines of chickens

Changes to daily feed intake during the laying period alters embryonicMSTNandMYOGgene expression in genetically fat and lean lines of chickens

Microarray analysis of genes differentially expressed in the liver of lean and fat chickens

Identification of genes involved in regulatory mechanism of pigments in broiler chickens

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