Profiling of different bioactive compounds in functional drinks by high-performance liquid chromatography

Abstract

12In the present work, an HPLC method is proposed to simultaneously detect and quantify 13 water and fat soluble vitamins, phenolic compounds, carotenoids and chlorophylls in a 14 single run, by using an ultradeactivated C18 column and gradient separation using 15 trifluoroacetic acid, water and methanol. It is shown that the HPLC method provides 16 baseline separation of all these compounds with good resolution values in 40 min. 17Moreover, other figures of merit of the method show a good linear response and low 18 detection limits for all the compounds considered in the present study. Furthermore, the 19 usefulness of this method is demonstrated via its successful application to the analysis 20 of different beverages from different natural origin (orange, strawberry, apple, peach 21 pineapple, plum and blackcurrant juices, soybean milk, beers) without the need of any 22 previous sample preparation. A good correlation is also found by comparing the total 23 phenol content (measured by Folin-Ciocalteau method) with the sum of total phenolic 24 compounds obtained using the proposed HPLC method. By using statistical tools, the 25 main compounds associated with antioxidant activity of the extracts (measured by 26 DPPH radical scavenging) were assessed. 27 can play an important role in the associated bioactivity of a food product and, therefore, 56 their determination can also be of great importance for food and nutrition studies. These 57 food components have the common property of being labile and so degraded by light, 58 heat or oxygen, also they are extremely complex, with dozens of very similar 59 components or isomers which makes its separation extremely difficult as next 60 discussed. 61The basic feature of all polyphenols is the presence of one or more hydroxylated 62 aromatic rings, which seemed to be, in fact, responsible for their properties as radical 63 scavengers [10][11][12][13][14][15][16] carotenoids are efficient free-radical scavengers, and they enhance the immune system 70 [20][21][22][23][24][25][26]. On the other hand, some biological effects have also been associated to 71 chlorophylls [20]. The analysis of carotenoids and chlorophylls is commonly done by 72 HPLC and several methods have been developed to simultaneously determine these 73 type of compounds [27][28][29]. 74The goal of this work was to develop an HPLC method (using diode array detection) 75 The antioxidant activity of beverages was measured using the DPPH method. All the 138 measurements were carried out according to a procedure previously used in juices by 139Klimczak et al. [30]. Briefly, 0.1 mL of juice sample (filtered using syringe nylon filter 140 of 0.45 μm and diluted with distilled water 10:1) was added to 2.46 mL of 1,1-diphenyl-141 2-picrylhydrazyl radical (DPPH  ; 0.025 g·L −1 in 50% ethanol) and mixed by vortex for 142 1 min. The absorbance of the samples was measured at 515 nm every 5 min for 30 min 143 using the spectrophotometer Genesys (Thermo Finnigan, Madrid, Spain). For each 144 sample, three separ...

Physicochemical and antioxidant properties of Bangladeshi honeys stored for more than one year

“…The color intensity of the honey samples ranged from 254 to 2034 mAU (Table 2), which is comparable to those reported by other authors [5,6,27,28]. …”

“…ABS 450 was used because it has previously been reported that anthocyanins, a class of flavonoids present in functional drinks [27], and carotenoids have absorption maxima at 450 nm. In this study, we found that three dark amber samples (BDH-4, 7 and 8) also contained high levels of phenolic and flavonoid compounds.…”

Antimicrobial Effect of Malpighia Punicifolia and Extension of Water Buffalo Steak Shelf‐Life

“…A modified HPLC method, previously developed in CIAL laboratory (Madrid, Spain) was used (Mendiola and others ). Shortly, analytes were separated on an ACE‐100 Å C18 (150 mm × 4.6 mm, 3 μm particle size; Advanced Chromatographic Technologies, Aberdeen, U.K.) in a single run, using combined isocratic and linear gradient elution with a mobile phase consisting of 0.010% TFA (solvent A, pH 3.9) and methanol (solvent B) at the flow rate of 0.7 mL/min using an Agilent 1100 HPLC apparatus.…”

“…The most suitable detection wavelength for simultaneous vitamin‐polyphenol determination was 280 nm. Quantification of the different families of compounds was performed by building a calibration curve for each of them, as described by Mendiola and others ().…”

Changes on phenolic and carotenoid composition of high intensity pulsed electric field and thermally treated fruit juice–soymilk beverages during refrigerated storage

“…It is well known that carotenoid distribution in citrus is extremely complex, with the largest number of carotenoids founded in any fruit (Meléndez-Martínez, Vicario, & Heredia, 2003). Fruit juices, drinks and mixed beverages have widely different carotenoid content, which mainly depend on the ingredients of the samples under study (Mendiola et al, 2008;. b-Carotene normally constitutes between 25% and 30% of total carotenoids in fruits (Bitton & Hornero-Méndez, 2001); however, unripe fruits usually show high contents of lutein (Gross, 1987).…”