Profiling of Differentially Expressed Genes in Peri-implantitis and Periodontitis in vivo by Microarray Analysis

Roediger, Matthias; Miró, Xavier; Geffers, Robert; Irmer, Malte; Huels, Alfons; Miosge, Nicolai; Gersdorff, Nikolaus

doi:10.1016/s1349-0079(09)80018-2

Cited by 5 publications

(3 citation statements)

References 41 publications

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“…Importantly, the leading-edge analyses revealed important differentially activated genes that are involved in multiple inflammations and immune responses related to biological processes or molecular pathways, including IL-1β, CDK3, IL-27 and CD86 in the FI vs. NI comparison, in addition to CXCL6, CXCL1, CCL7, CCL13 and IL18 in the SI vs. NI comparison. Previous transcriptomics studies profiling the differentially expressed genes in peri-implantitis have pointed out that the significantly altered pathways were related to extracellular matrix molecules [26], matrix-degrading enzymes [27] and inflammatory pathways including the RANKL/OPG pathway and the cyclooxygenase2 pathway [28].…”

Section: Discussionmentioning

confidence: 99%

Titanium Particles Modulate Lymphocyte and Macrophage Polarization in Peri-Implant Gingival Tissues

Kheder

Bouzid

Venkatachalam

et al. 2023

IJMS

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Titanium dental implants are one of the modalities to replace missing teeth. The release of titanium particles from the implant’s surface may modulate the immune cells, resulting in implant failure. However, little is known about the immune microenvironment that plays a role in peri-implant inflammation as a consequence of titanium particles. In this study, the peri-implant gingival tissues were collected from patients with failed implants, successful implants and no implants, and then a whole transcriptome analysis was performed. The gene set enrichment analysis confirmed that macrophage M1/M2 polarization and lymphocyte proliferation were differentially expressed between the study groups. The functional clustering and pathway analysis of the differentially expressed genes between the failed implants and successful implants versus no implants revealed that the immune response pathways were the most common in both comparisons, implying the critical role of infiltrating immune cells in the peri-implant tissues. The H&E and IHC staining confirmed the presence of titanium particles and immune cells in the tissue samples, with an increase in the infiltration of lymphocytes and macrophages in the failed implant samples. The in vitro validation showed a significant increase in the level of IL-1β, IL-8 and IL-18 expression by macrophages. Our findings showed evidence that titanium particles modulate lymphocyte and macrophage polarization in peri-implant gingival tissues, which can help in the understanding of the imbalance in osteoblast–osteoclast activity and failure of dental implant osseointegration.

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Section: Discussionmentioning

confidence: 99%

Titanium Particles Modulate Lymphocyte and Macrophage Polarization in Peri-Implant Gingival Tissues

Kheder

Bouzid

Venkatachalam

et al. 2023

IJMS

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“…Because bacteria and i-TiPs require similar membrane internalization events, it is not possible to disaggregate differences due to endocytosis and intracellular processing of i-TiPs in peri-implant disease. One study comparing transcriptome expression patterns between peri-implantitis and periodontitis showed that certain genes involved in actin regulation, endosomal processing, and antigen presentation are expressed differently between the groups, furthering the idea that upregulation of these processes may be unique to peri-implantitis (Roediger et al 2009). These findings are crucial because some compounds (CK-666, CK-869) have been shown to inhibit Arp2/3 actin nucleation activity in vitro and thus potentially function to reduce inflammation and other pathology instigated by i-TiPs in peri-implantitis (Hetrick et al 2013).…”

Section: Discussionmentioning

confidence: 99%

“…We identified gene expression differences between groups that pinpoint molecules and processes that may be responsible for the inflammation and bone degradation related to the endosomal-lysosomal pathway. Existing transcriptomics studies have pointed to altered gene expression pathways related to extracellular matrix molecules (Roediger et al 2009), matrixdegrading enzymes (Roediger et al 2009;Cho et al 2020), and inflammatory pathways such as the cyclooxygenase 2 pathway (Liu et al 2020) and the RANKL/OPG pathway (Becker et al 2014;Liu et al 2020). Importantly, when Becker et al ( 2014) compared genome-wide transcriptome profiles of 7 patients with peri-implantitis versus 7 with periodontitis, they observed drastic The top of the chart signifies relatedness of individual samples, with bottom groups showing the highest degree of similarity and top groups showing less similarity.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome-wide Gene Expression Analysis in Peri-implantitis Reveals Candidate Cellular Pathways

Martin

Zhou

Singh

et al. 2021

JDR Clinical & Translational Research

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Objective: Peri-implantitis is a condition resulting in destructive inflammation in the peri-implant soft tissue barrier. Clinically, it demonstrates vast clinical differences to periodontitis that suggest distinct inflammatory mechanisms. Implant-derived titanium particles (i-TiPs) frequently found around diseased implants appear to alter the microenvironment and confer resistance to antibiotic treatments. Studies in orthopedic implants have demonstrated potent inflammatory responses to i-TiPs involving a variety of cell types in aseptic conditions. Nonetheless, the genetic programs of cells surveilling and supporting the peri-implant soft tissue barrier in response to the combined challenges of biomaterial degradation products and oral bacteria are poorly defined. Thus, we studied gene expression specific to oral peri-implant inflammatory disease. Methods: Peri-implant tissues were collected from healthy or diseased implants (N = 10) according to the 2018 classification criteria. Following RNA extraction and purification, a gene-level view of the transcriptome was obtained via a next-generation transcriptome-wide microarray profiling workflow (Clariom S; Applied Biosystems) that covers >20,000 well-annotated genes. A discovery analysis assessed global differential expression of genes and identified pathways in peri-implant health versus disease. Results: Genes involved in the endosomal-lysosomal pathway, such as actin polymerization, were strongly upregulated in diseased tissues (P < .05), proposing increased intracellular activities in response to bacteria and i-TiPs. Cellular respiration pathways involved in oxidative stress were highly transcribed in all peri-implant samples, suggesting that implant-specific factors may trigger a constant state of oxidative stress. Conclusion: Within the limitations of this discovery study, expressive upregulation of genes in the endosomal-lysosomal and oxidative stress pathway suggests that inflammation related to receptor-driven responses to extracellular signals, such as i-TiPs and pathogens, may have a crucial role in peri-implantitis. Results warrant external replication in validation cohorts. Knowledge Transfer Statement: Our findings regarding physiologic processes affected by peri-implantitis could advance knowledge of the mechanisms and consequences of the disease. Understanding the cellular programs that partake in peri-implant inflammation has the potential to translate to novel treatment strategies for patients with peri-implantitis.

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Dental implant material related changes in molecular signatures in peri-implantitis – A systematic review and integrative analysis of omics in-vitro studies

et al. 2023

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Profiling of Differentially Expressed Genes in Peri-implantitis and Periodontitis in vivo by Microarray Analysis

Cited by 5 publications

References 41 publications

Titanium Particles Modulate Lymphocyte and Macrophage Polarization in Peri-Implant Gingival Tissues

Titanium Particles Modulate Lymphocyte and Macrophage Polarization in Peri-Implant Gingival Tissues

Transcriptome-wide Gene Expression Analysis in Peri-implantitis Reveals Candidate Cellular Pathways

Dental implant material related changes in molecular signatures in peri-implantitis – A systematic review and integrative analysis of omics in-vitro studies

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