Profiling of DNA damage and repair pathways in small cell lung cancer reveals a suppressive role in the immune landscape

Jin, Renjing; Liu, Bin; Yu, Mengjun; Song, Lan; Gu, Meng; Wang, Ziyu; Li, Xiaobo; Zhang, Xu; Wang, Jinghui; Ma, Teng

doi:10.1186/s12943-021-01432-5

Cited by 11 publications

(8 citation statements)

References 15 publications

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“…Furthermore, Jin et al. ( 36 ) explored the relationship between repair of DNA damage and the immune microenvironment in small cell lung carcinoma. They found that homologous DNA pairing and chain exchange links in DNA double strand break homologous recombination repair correlated negatively with the immune microenvironment in small cell lung carcinoma.…”

Section: Resultsmentioning

confidence: 99%

“…Lin et al (27) divided liver cancer patients into DDR-activated subtype and DDR-suppressed subtype according to various clinical and molecular characteristics, and the results of single-cell sequencing showed a significant difference in the number of T cells between the two groups. Furthermore, Jin et al (36) explored the relationship between repair of DNA damage and the immune microenvironment in small cell lung carcinoma. They found that homologous DNA pairing and chain exchange links in DNA double strand break homologous recombination repair correlated negatively with the immune microenvironment in small cell lung carcinoma.…”

Section: Analysis Of Research Using Single-cell Sequencing On Dna Dam...mentioning

confidence: 99%

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Bibliometric analysis of single-cell sequencing researches on immune cells and their application of DNA damage repair in cancer immunotherapy

Zhao

Xiao

et al. 2023

Front. Oncol.

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IntroductionIn recent decades, single-cell sequencing technology has developed rapidly and used widely in various fields of life sciences, especially for the detection of immune cells. A bibliometric analysis of single-cell sequencing research work on immune cells published during the 2011-2021 period should provide new insight on the use of single-cell sequencing.MethodsWe screened 1,460 publications on single-cell sequencing on immune cells according to the publication date, article type, language, and country.ReultsThe United States published the first and largest number of articles, while China’s research started relatively late, but ranked second in the number of publications. T cells were the most commonly studied immune cells by single-cell sequencing, followed by mononuclear macrophages. Cancer biology was the most common field of immune cell research by single-cell sequencing. Single-cell sequencing studies using γδ T cells were mainly in the fields of cancer biology and cell development, and focused over time from cell surface receptor to cell function. Through in-depth analysis of the articles on single-cell sequencing of T cells in the oncology field, our analysis found that immunotherapy and tumor microenvironment were the most popular research directions in recent years.DiscussionThe combination of DNA damage repair and immunotherapy seems to provide a new strategy for cancer therapy.

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Section: Resultsmentioning

confidence: 99%

Section: Analysis Of Research Using Single-cell Sequencing On Dna Dam...mentioning

confidence: 99%

Bibliometric analysis of single-cell sequencing researches on immune cells and their application of DNA damage repair in cancer immunotherapy

Zhao

Xiao

et al. 2023

Front. Oncol.

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“…In addition to PD-L1, the identification of other accurate and predictive biomarkers is still essential for the selection of appropriate ICI treatment. The preferred treatment for NSCLC is radiotherapy and/or cisplatin chemotherapy; however, a large number of patients diagnosed with NSCLC are at risk of organ metastasis and resistance to radiotherapy/chemotherapy, leading to treatment failure [17]. Therefore, it is necessary to further study the causes of lung cancer treatment failure and provide a more accurate basis for lung cancer treatment.…”

Section: Discussionmentioning

confidence: 99%

Study on the expression of RAD51 in non-small cell lung cancer based on bioinformatics

WU¹,

ZHANG

ZHANG³

et al. 2023

Preprint

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Objective RAD51 is a DNA repair protein, which participates in the resistance of tumor cells to radiotherapy/chemotherapy and reduces the therapeutic effect. Based on the research status of RAD51 at home and abroad and the analysis of online databases, the purpose of this study was to explore the relationship between RAD51 expression and clinical patient survival and prognosis. It is expected to provide a new theoretical basis for the clinical treatment of lung cancer patients, help identify new molecular markers, and provide new targets for the biological therapy of lung cancer patients. Methods the RNA Seq data of NSCLC in TCGA database were downloaded, and the expression of RAD51 gene in NSCLC and normal tissues were analyzed by R studio software. Clinical correlation analysis revealed its correlation with the clinicopathological characteristics of non-small cell lung cancer. Survival analysis was used to evaluate the relationship between the expression level and the prognosis of patients. CIBERSORT and TIMER were used to evaluate the correlation between the expression level of CIBERSORT and immune cells in the tumor microenvironment. The protein expression level of RAD51 in non-small cell lung cancer was evaluated by HPA. Results RAD51 was highly expressed in lung cancer (p<0.05), which was significantly associated with poor prognosis of lung adenocarcinoma patients (p=0.0026), but not with lung squamous cell carcinoma (p=0.76). The expression level of RAD51 mRNA was associated with different pathological stages of lung adenocarcinoma (p=0.000528), but not with different pathological stages of lung squamous cell carcinoma (p=0.326). RAD51 was positively correlated with the expression of TP53, BRAF, EGFR, MYC, PD-L1, and KRAS (p<0.001). In lung adenocarcinoma, lung cancer cells were positively correlated with CD4+memory T cells, CD8+T cells, and M1 macrophages (p<0.001). In lung squamous cell carcinoma, tumor cells were positively correlated with M1 macrophages (p<0.05), but not with CD4+T memory cells, CD8+T cells, M2 macrophages, and Tregs cells (p>0.05). The HPA database indicated that RAD51 protein was positive in non-small cell lung cancer. Conclusion RAD51 is highly expressed in non-small cell lung cancer and is associated with poor prognosis. RAD51 can be used as a biomarker related to the prognosis of non-small cell lung cancer and is expected to become a target for the diagnosis and treatment of non-small cell lung cancer.

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“…However, the role of MDA5-MAVS pathway in tumor immunity is still very complex, and many questions remain unanswered. Recently, increasing evidences have illustrated how the innate immune pathway in the tumor microenvironment changes tumorigenesis in different tumors, thus affecting the subsequent design of effective immunotherapy trials ( 83 , 84 ).…”

Section: Discussionmentioning

confidence: 99%

The intersection molecule MDA5 in Cancer and COVID-19

Jin

Cao

et al. 2022

Front. Immunol.

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The connections between pattern recognition receptors (PRRs) and pathogen-associated molecular patterns (PAMPs) constitutes the crucial signaling pathways in the innate immune system. Cytoplasmic nucleic acid sensor melanoma differentiation-associated gene 5 (MDA5) serves as an important pattern recognition receptor in the innate immune system by recognizing viral RNA. MDA5 also plays a role in identifying the cytoplasmic RNA from damaged, dead cancer cells or autoimmune diseases. MDA5’s recognition of RNA triggers innate immune responses, induces interferon (IFN) response and a series of subsequent signaling pathways to produce immunomodulatory factors and inflammatory cytokines. Here we review the latest progress of MDA5 functions in triggering anti-tumor immunity by sensing cytoplasmic dsRNA, and recognizing SARS-CoV-2 virus infection for antiviral response, in which the virus utilizes multiple ways to evade the host defense mechanism.

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Profiling of DNA damage and repair pathways in small cell lung cancer reveals a suppressive role in the immune landscape

Cited by 11 publications

References 15 publications

Bibliometric analysis of single-cell sequencing researches on immune cells and their application of DNA damage repair in cancer immunotherapy

Bibliometric analysis of single-cell sequencing researches on immune cells and their application of DNA damage repair in cancer immunotherapy

Study on the expression of RAD51 in non-small cell lung cancer based on bioinformatics

The intersection molecule MDA5 in Cancer and COVID-19

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