Profiling of Heterobranchia Sea Slugs from Portuguese Coastal Waters as Producers of Anti-Cancer and Anti-Inflammatory Agents

Gomes, Nelson G.M.; Fernandes, Francisco Maximino; Madureira-Carvalho, Áurea; Valentão, Patrı́cia; Lobo-da-Cunha, Alexandre; Calado, Gonçalo; Andrade, Paula B.

doi:10.3390/molecules23051027

Cited by 12 publications

(11 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…TCP FAs, 24:5n-6 and 24:6n-3, found in high proportions (each is more than 10% of total FAs) in the tritonid nudibranch Tochuina tetraquetra, originate undoubtedly from soft corals of the subclass Alcyonaria, which it feeds on [73]. FAs of the nudibranch Armina maculate, which feeds on a pennatulacean commonly named "Sea Pen", Veretillum cynomorium, constituted predominantly 16:0, 18:0, 20:4n-6 and 20:5n-3 (62% of total FA); thus, evidencing a similarity with the FA profile of "Sea Pen" represented by the same major compounds, whereas FAs of the cephalaspidean Aglaja tricolorata, presumably feeding on foraminiferans from sandy bottoms, is rich in EPA and DHA (27% of total FAs) [81]. Moreover, the studied nudibranchs exhibit one more specific feature: their lipids are rich in n-6 PUFA, and their level is much higher than that of n-3 PUFAs.…”

Section: Gastropodamentioning

confidence: 96%

Fatty Acids of Marine Mollusks: Impact of Diet, Bacterial Symbiosis and Biosynthetic Potential

Zhukova

2019

Biomolecules

View full text Add to dashboard Cite

The n-3 and n-6 polyunsaturated fatty acid (PUFA) families are essential for important physiological processes. Their major source are marine ecosystems. The fatty acids (FAs) from phytoplankton, which are the primary producer of organic matter and PUFAs, are transferred into consumers via food webs. Mollusk FAs have attracted the attention of researchers that has been driven by their critical roles in aquatic ecology and their importance as sources of essential PUFAs. The main objective of this review is to focus on the most important factors and causes determining the biodiversity of the mollusk FAs, with an emphasis on the key relationship of these FAs with the food spectrum and trophic preference. The marker FAs of trophic sources are also of particular interest. The discovery of new symbioses involving invertebrates and bacteria, which are responsible for nutrition of the host, deserves special attention. The present paper also highlights recent research into the molecular and biochemical mechanisms of PUFA biosynthesis in marine mollusks. The biosynthetic capacities of marine mollusks require a well-grounded evaluation.Biomolecules 2019, 9, 857 2 of 25 consumers and food consumed, and, therefore, FA can be used as efficient and useful biomarkers for the study of trophic interactions between organisms in aquatic ecosystems [5,6].However, information about the endogenous mechanisms of marine invertebrates responsible for synthesis of n-3 and n-6 PUFAs is still being accumulated. Recent researches have shown the potential of some marine mollusks for endogenous synthesis of long chain PUFAs (LC-PUFAs) [7][8][9]. Based on the transcriptome and genome sequences, as well as various publicly available databases, a number of novel fatty acyl desaturases (Fad) and elongations of very long-chain fatty acid (Elovl) genes have been identified from the major orders of the phylum Mollusca, suggesting that many mollusks possess most of the required enzymes for the synthesis of long chain LC-PUFAs [10]. The question whether these findings of the desaturase sequences in invertebrate species really cast doubt on the idea that the organic matter is transferred along the food chains, and thus the existence of trophic links between primary producers and consumers and the relationship of the FA composition of animals and the FA composition of food, are currently under discussion [8].Mollusk FA have attracted the attention of researchers that has been driven by their critical roles in aquatic ecology and in trophic food webs, as well as by their importance as sources of essential FAs with important impacts on human health [11]. Among marine animals, mollusks are especially important as a source of PUFAs (after fish). Many members of the phylum Mollusca, commonly known as clams and snails, are traditional seafood items in human diets, and rich in essential PUFAs. The edible mollusks are commercially harvested and cultured [12]. Marine bivalve mollusks are highly appreciated, partly because of their positive effects on human health ...

show abstract

Section: Gastropodamentioning

confidence: 96%

Fatty Acids of Marine Mollusks: Impact of Diet, Bacterial Symbiosis and Biosynthetic Potential

Zhukova

2019

Biomolecules

View full text Add to dashboard Cite

show abstract

“…Each taxonomic group of nudibranchs’ preys is characterized by specific low-molecular weight compounds, which are consumed by nudibranchs and can be recognized in their tissues 12,13 . Lipids are the most important part of the low-molecular weight compounds in the diet of consumers and cover a considerable part of its energy demands.…”

Section: Introductionmentioning

confidence: 99%

Lipidomic study of the influence of dietary fatty acids on structural lipids of cold-water nudibranch molluscs

Imbs

Grigorchuk

2019

Sci Rep

View full text Add to dashboard Cite

Nudibranch molluscs occur in marine ecosystems worldwide and prey on numerous invertebrate species. During feeding, dietary fatty acids (FAs) unusual for nudibranchs are transferred to their lipids. Normal biomembrane functions require stable composition of structural polar lipids (PL), but the pathways of dietary FA utilization to PL in nudibranchs still remain unknown. A combination of chromatography and tandem high-resolution mass spectrometry was used to determine total lipid, PL, FA, and PL molecular species composition of two cold-water species of Dendronotus, which then were compared with those of Tritonia tetraquetra. The use of FA trophic markers showed that Dendronotus sp. and T. tetraquetra prey on different soft corals, while D. robustus may consumes hydrocorals and bryozoans. Nudibranch FA profiles were strongly modified by dietary FAs but their PL profilers were similar. Dietary FAs are not included in ceramide aminoethylphosphonate and inositol glycerophospholipids, but directed to ethanolamine, choline, and serine glycerophospholipids and, in some cases, form isobaric molecular species with different FA chain lengths. For such isobaric species, nudibranchs reduce the length of alkyl groups when very-long-chain FAs are obtained with diet. This molecular mechanism may explain the adaptation of nudibranch membrane structure to dietary input of unusual FAs.

show abstract

“…Then the cells were subcultured in 96-well plates (1.5 × 10 4 cells well −1 ), grown until 80% confluence, and subsequently exposed to the extract for 24 h at 37 °C. Interference with the mitochondrial activity was studied 24 h later by the MTT reduction assay as described in Gomes et al [50]. Viable cells were calculated as a percentage of the negative control cells set at 100%, results corresponding to the mean ± SEM of three independent experiments performed in triplicate.…”

Section: Methodsmentioning

confidence: 99%

Flavonoid Composition of Salacia senegalensis (Lam.) DC. Leaves, Evaluation of Antidermatophytic Effects, and Potential Amelioration of the Associated Inflammatory Response

et al. 2019

Self Cite

View full text Add to dashboard Cite

Predominantly spread in West Tropical Africa, the shrub Salacia senegalensis (Lam.) DC. is known because of its medicinal properties, the leaves being used in the treatment of skin diseases. Prompted by the ethnomedicinal use, a hydroethanolic extract obtained from the leaves of the plant was screened against a panel of microbial strains, the majority of which involved in superficial infections. The extract was found to be active against the dermatophytes Trichophyton rubrum and Epidermophyton floccosum. Notable results were also recorded regarding the attenuation of the inflammatory response, namely the inhibitory effects observed against soybean 5-lipoxygenase (IC50 = 71.14 μg mL−1), no interference being recorded in the cellular viability of RAW 264.7 macrophages and NO levels. Relevantly, the extract did not lead to detrimental effects against the keratinocyte cell line HaCaT, at concentrations displaying antidermatophytic and anti-inflammatory effects. Flavonoid profiling of S. senegalensis leaves was achieved for the first time, allowing the identification and quantitation of myricitrin, three 3-O-substituted quercetin derivatives, and three other flavonoid derivatives, which may contribute, at least partially, to the observed antidermatophytic and anti-inflammatory effects. In the current study, the plant S. senegalensis is assessed concerning its antidermatophytic and anti-inflammatory properties.

show abstract

Profiling of Heterobranchia Sea Slugs from Portuguese Coastal Waters as Producers of Anti-Cancer and Anti-Inflammatory Agents

Cited by 12 publications

References 48 publications

Fatty Acids of Marine Mollusks: Impact of Diet, Bacterial Symbiosis and Biosynthetic Potential

Fatty Acids of Marine Mollusks: Impact of Diet, Bacterial Symbiosis and Biosynthetic Potential

Lipidomic study of the influence of dietary fatty acids on structural lipids of cold-water nudibranch molluscs

Flavonoid Composition of Salacia senegalensis (Lam.) DC. Leaves, Evaluation of Antidermatophytic Effects, and Potential Amelioration of the Associated Inflammatory Response

Contact Info

Product

Resources

About