Profiling of HSPB9 gene sequences in Donggala (Bos indicus) and Polled Bali (Bos javanicus) cattle breeds of Indonesia

Abstract

HSPB9 gene plays important role to protect cells from heat stress in mammals. This research aim to describe the DNA sequence of HSPB9 gene in Donggala (Bos indicus) and Bali (Bos javanicus) bulls using forward sequencing method. A total of 10 blood samples from 6 Donggala and 4 Polled Bali were used in this study for DNA analysis. The bulls were kept at the breeding station at Central Sulawesi and South Sulawesi for Donggala and Bali, respectively. Results showed that a novel nonsence mutation of c.216C>T (p.L68L) was detected in the exon 1 of Bali HSPB9 gene. Commonly, the HSPB9 gene was monomorphic in Donggala and Bali cattle. In conclusion, the mutation of c.216C>T might be used as the genetic marker to discriminate Donggala and Bali cattle.