2010
DOI: 10.1007/s00213-010-2032-3
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Profiling of hypothalamic and hippocampal gene expression in chronically stressed rats treated with St. John’s wort extract (STW 3-VI) and fluoxetine

Abstract: In conclusion, microarray analysis proved to be a valuable tool to identify a large number of genes and resulting pathways that may serve as novel drug targets or predict drug responsiveness for SJW or fluoxetine. Based on our comprehensive analysis, it was possible to identify similarities and differences between SJW and fluoxetine which may help to better understand their molecular action and, in addition, help to find novel treatment strategies for stress-related depression.

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Cited by 28 publications
(15 citation statements)
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“…Proteins such as ALDOAA, PGAM1B, PHB2, SLC25A5, SNCB, TPI1B, VDAC3, alpha globin, PPIAB and IDH2 were found to be upregulated, whereas ALDOCB, CKM, EEF1A, CH211236K19.6, NME2B, PVALB5 and CKB were found to be downregulated relative to their levels in control brain samples (Table 4). We found that some proteins, such as aldolase C, synuclein B and PVALB2, which have been implicated in mood disorders in rodents [34][36] and in other psychiatric disorders in humans [37][39], were also dysregulated in zebrafish. The interesting protein ependymin (EP), a fish neurotrophin involved in neuroplasticity, was also observed in our proteome data; it was down-regulated (1.5-fold) in the brains of fish exhibiting CUS-induced anxiety and related disorders.…”
Section: Resultsmentioning
confidence: 87%
“…Proteins such as ALDOAA, PGAM1B, PHB2, SLC25A5, SNCB, TPI1B, VDAC3, alpha globin, PPIAB and IDH2 were found to be upregulated, whereas ALDOCB, CKM, EEF1A, CH211236K19.6, NME2B, PVALB5 and CKB were found to be downregulated relative to their levels in control brain samples (Table 4). We found that some proteins, such as aldolase C, synuclein B and PVALB2, which have been implicated in mood disorders in rodents [34][36] and in other psychiatric disorders in humans [37][39], were also dysregulated in zebrafish. The interesting protein ependymin (EP), a fish neurotrophin involved in neuroplasticity, was also observed in our proteome data; it was down-regulated (1.5-fold) in the brains of fish exhibiting CUS-induced anxiety and related disorders.…”
Section: Resultsmentioning
confidence: 87%
“…Studies based on microarray analyses of gene expression following antidepressant treatment usually compare region-specific gene expression changes either after different ADs treatment (Altar et al 2004; Boehm et al 2006; Conti et al 2007; Drigues et al 2003; Jungke et al 2011; Knuuttila et al 2004; Palotas et al 2004; Ploski et al 2006; Sillaber et al 2008; Takahashi et al 2006; Wong et al 2004) or in different animal models of depression (Andrus et al 2010; Bergstrom et al 2007; Orsetti et al 2008; Surget et al 2009). In our study, we broadened the perspective by evaluating the time-course of gene expression changes during antidepressant treatment.…”
Section: Discussionmentioning
confidence: 99%
“…Six animals from each experimental group were used for microarray analysis. To reduce noise and individual variability and increase statistical power (Krishnan et al,2007; Jungke et al,2011), two total RNA sample, processed as described above, were pooled to obtain a unique sample to be hybridized on a single Gene Chip Rat Exon Array 1.0 (henceforth referred to as Exon Array ; Affymetrix, Santa Clara, CA). We processed 1 μg of total RNA to sample with the GeneChip Whole Transcript (WT) Sense Target Labeling Assay (Affymetrix), according to the manufacturer's protocol, to hybridize on GeneChip Rat Exon 1.0 Array.…”
Section: Methodsmentioning
confidence: 99%