Profiling of Plant Derived Natural Constituents by Using Magnetic Resonance Techniques

Anand, Anupama; Sharma, Anshu; Saini, Harpreet Kaur; Sharma, Somesh; Sharma, Ruchi; Thakur, Chahat; Priyanka,; Atanassova, Maria; Caruso, Gianluca; Pasdaran, Ardalan

doi:10.1155/2022/5705637

Cited by 5 publications

(8 citation statements)

References 95 publications

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“…However, NMR requires several milligrams of the compound and is difficult to achieve by Prep‐TLC 44 . Although 1–2 mg may be enough for NMR (600 MHz), it is suggested to use more than 5 mg to identify an unknown compound, due to its low sensitivity 45 . However, nowadays, the use of molecular networking that connects mass spectra based on the similarity of their fragmentation patterns 46 allows an efficient and reliable identification with a small amount of sample (< 1 mg).…”

Section: Resultsmentioning

confidence: 99%

“…44 Although 1-2 mg may be enough for NMR (600 MHz), it is suggested to use more than 5 mg to identify an unknown compound, due to its low sensitivity. 45 However, nowadays, the use of molecular networking that connects mass spectra based on the similarity of their fragmentation patterns 46 allows an efficient and reliable identification with a small amount of sample (< 1 mg). Thus, some web databases, such as GNPS, MoNA, and FooDB, are open access and facilitate the identification step considerably.…”

Section: Identification By Uhplc-ms/ms Analysismentioning

confidence: 99%

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Application of effect‐directed analysis using TLC–bioautography for rapid isolation and identification of antidiabetic compounds from the leaves of Annona cherimola Mill.

Galarce‐Bustos,

Obregón,

Vallejos‐Almirall

et al. 2023

Phytochemical Analysis

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IntroductionType 2 diabetes mellitus is a globally prevalent chronic disease characterised by hyperglycaemia and oxidative stress. The search for new natural bioactive compounds that contribute to controlling this condition and the application of analytical methodologies that facilitate rapid detection and identification are important challenges for science. Annona cherimola Mill. is an important source of aporphine alkaloids with many bioactivities.ObjectiveThe aim of this study is to isolate and identify antidiabetic compounds from alkaloid extracts with α‐glucosidase and α‐amylase inhibitory activity from A. cherimola Mill. leaves using an effect‐directed analysis by thin‐layer chromatography (TLC)–bioautography.MethodologyGuided fractionation for α‐glucosidase and α‐amylase inhibitors in leaf extracts was done using TLC–bioassays. The micro‐preparative TLC was used to isolate the active compounds, and the identification was performed by mass spectrometry associated with web‐based molecular networks. Additionally, in vitro estimation of the inhibitory activity and antioxidant capacity was performed in the isolated compounds.ResultsFive alkaloids (liriodenine, dicentrinone, N‐methylnuciferine, anonaine, and moupinamide) and two non‐alkaloid compounds (3‐methoxybenzenepropanoic acid and methylferulate) with inhibitory activity were isolated and identified using a combination of simple methodologies. Anonaine, moupinamide, and methylferulate showed promising results with an outstanding inhibitory activity against both enzymes and antioxidant capacity that could contribute to controlling redox imbalance.ConclusionsThese high‐throughput methodologies enabled a rapid isolation and identification of seven compounds with potential antidiabetic activity. To our knowledge, the estimated inhibitory activity of dicentrinone, N‐methylnuciferine, and anonaine against α‐glucosidase and α‐amylase is reported here for the first time.

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Section: Resultsmentioning

confidence: 99%

Section: Identification By Uhplc-ms/ms Analysismentioning

confidence: 99%

Application of effect‐directed analysis using TLC–bioautography for rapid isolation and identification of antidiabetic compounds from the leaves of Annona cherimola Mill.

Galarce‐Bustos,

Obregón,

Vallejos‐Almirall

et al. 2023

Phytochemical Analysis

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“…One major concern about natural colorant and pigments is their quality and purity. Different hyphenated techniques and equipment, including HPLC (high‐performance liquid chromatography), HPTLC (high‐performance thin‐layer chromatography), GC‐MS (gas chromatography‐mass spectrometry), LC–MS (liquid chromatography‐mass spectrometry), and NMR (nuclear magnetic resonance), may be applied for quality control of natural products [809–812] . Moreover, ICP‐MS (inductively coupled plasma mass spectrometry) and AAS (atomic absorption spectroscopy) can be applied to detect and quantify the presence of heavy metals in natural colorant extracts, drugs, and cosmetics [813–815] …”

Section: Resultsmentioning

confidence: 99%

“…Different hyphenated techniques and equipment, including HPLC (high-performance liquid chromatography), HPTLC (high-performance thin-layer chromatography), GC-MS (gas chromatography-mass spectrometry), LC-MS (liquid chromatography-mass spectrometry), and NMR (nuclear magnetic resonance), may be applied for quality control of natural products. [809][810][811][812] Moreover, ICP-MS (inductively coupled plasma mass spectrometry) and AAS (atomic absorption spectroscopy) can be applied to detect and quantify the presence of heavy metals in natural colorant extracts, drugs, and cosmetics. [813][814][815] It is essential to consider that there are limitations on solvent residues and the type of solvents that are allowed to be used for extraction of the colorants and pigments.…”

Section: Phytoglobins and Peptides (Soy Leghemoglobin)mentioning

confidence: 99%

A Review of the Chemistry and Biological Activities of Natural Colorants, Dyes, and Pigments: Challenges, and Opportunities for Food, Cosmetics, and Pharmaceutical Application

Pasdaran

Zare

Hamedi

et al. 2023

Chemistry & Biodiversity

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Natural pigments are important sources for the screening of bioactive lead compounds. This article reviewed the chemistry and therapeutic potentials of over 570 colored molecules from plants, fungi, bacteria, insects, algae, and marine sources. Moreover, related biological activities, advanced extraction, and identification approaches were reviewed. A variety of biological activities, including cytotoxicity against cancer cells, antioxidant, anti‐inflammatory, wound healing, anti‐microbial, antiviral, and anti‐protozoal activities, have been reported for different pigments. Considering their structural backbone, they were classified as naphthoquinones, carotenoids, flavonoids, xanthones, anthocyanins, benzotropolones, alkaloids, terpenoids, isoprenoids, and non‐isoprenoids. Alkaloid pigments were mostly isolated from bacteria and marine sources, while flavonoids were mostly found in plants and mushrooms. Colored quinones and xanthones were mostly extracted from plants and fungi, while colored polyketides and terpenoids are often found in marine sources and fungi. Carotenoids are mostly distributed among bacteria, followed by fungi and plants. The pigments isolated from insects have different structures, but among them, carotenoids and quinone/xanthone are the most important. Considering good manufacturing practices, the current permitted natural colorants are: Carotenoids (canthaxanthin, β‐carotene, β‐apo‐8′‐carotenal, annatto, astaxanthin) and their sources, lycopene, anthocyanins, betanin, chlorophyllins, spirulina extract, carmine and cochineal extract, henna, riboflavin, pyrogallol, logwood extract, guaiazulene, turmeric, and soy leghemoglobin.

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“…Furthermore, the comparison of metabolomic profiling on open-field cultivation versus greenhouse-grown crops requires detailed and in-depth exploration. Plants are a rich source of metabolites that are used in medicines, food additives, nutraceuticals, flavourings, and other commercial applications [ 19 ]. The small molecules, intermediates, and compounds produced during metabolism are known as metabolites.…”

Section: Introductionmentioning

confidence: 99%

1H NMR-Based Metabolomics Profile of Green and Red Amaranthus Grown in Open Field versus Greenhouse Cultivation System

Nemadodzi,

Managa

2023

Metabolites

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Traditionally, indigenous African leafy vegetables such as Amaranthus, blackjack, jute mallow, cleome monophyla, and spider plants have been conventionally and organically grown as weeds in open fields. However, the lack of land space due to the increase in population has resulted in unconventional, modern, and advanced agricultural farming. The introduction of a greenhouse has recently become the second most popular growing system alongside shade net and glasshouse to increase productivity and meet consumers’ demand. Several studies on Amaranthus species have solely focused on physiological parameters and nutritional composition, leaving a huge gap on their metabolomic profile of the leaves which is crucial to comprehend when growing Amaranthus species in different cropping systems. Therefore, the study aimed to determine the influence of different cropping systems on the release of metabolites of two commonly consumed Amaranthus species in South Africa. H1 -Nuclear Magnetic Resonance (NMR) tool was used to profile the untargeted metabolites of green (Amaranthus graecizans L.) and red (Amaranthus cruentus L.) species. A total of 12 metabolites—trehalose, betaine, glutamine, choline, sucrose, caprate, adenosine, asparagine, carnitine, caffeine, aspartate, and alanine—were detected in green amaranth grown in open fields. Except for caffeine, aspartate, and caprate, which were found in the green amaranth grown in open fields, all the other metabolites were detected in the greenhouse grown once. Interestingly, allantoin, which serves as an allelochemical, was the sole distinct metabolite detected in greenhouse cultivated green amaranth. On the contrary, seven similar metabolites were quantified in red amaranth grown in both open fields and greenhouses, apart from caffeine, which was only detected in greenhouse-cultivated red amaranth.

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Profiling of Plant Derived Natural Constituents by Using Magnetic Resonance Techniques

Cited by 5 publications

References 95 publications

Application of effect‐directed analysis using TLC–bioautography for rapid isolation and identification of antidiabetic compounds from the leaves of Annona cherimola Mill.

Application of effect‐directed analysis using TLC–bioautography for rapid isolation and identification of antidiabetic compounds from the leaves of Annona cherimola Mill.

A Review of the Chemistry and Biological Activities of Natural Colorants, Dyes, and Pigments: Challenges, and Opportunities for Food, Cosmetics, and Pharmaceutical Application

1H NMR-Based Metabolomics Profile of Green and Red Amaranthus Grown in Open Field versus Greenhouse Cultivation System

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