Profiling of Polyphenolic Compounds of Leontopodium alpinum Cass Callus Cultures Using UPLC/IM-HRMS and Screening of In Vitro Effects

Abstract

Leontopodium alpinum Cass. (edelweiss) is recognized as a frequent constituent of anti-aging skin care products, providing increased antioxidant and anti-inflammatory defense. Considering the growing demand and the protected status of edelweiss in many countries, alternative methods of production have been developed, one of them being callus culturing. This study reports the phytochemical composition of a methanolic extract of L. alpinum callus cultures, characterized by liquid chromatography coupled to ion-mobility high resolution mass spectrometry (UPLC/IM-HRMS). The methanolic extract exhibited strong free radical scavenging activity (122.19 ± 7.28 mg AAE/g dw), while the quantitative evaluation revealed that four major constituents (phenylpropanoid derivatives) represent 57.13% (m/m) of the extract. Consequently, a screening of antiproliferative effects was performed on ten cancer cell lines, representative of prostate, colon, lung and breast cancer, showing inhibition of colony formation in all cases. These results provide a comprehensive phytochemical characterization of L. alpinum callus cultures using advanced IM-HRMS, while the in vitro explorations confirmed the potent antioxidant properties of edelweiss which are worth exploring further in cancer prevention.

Improvement in Yield of Extracellular Vesicles Derived from Edelweiss Callus Treated with LED Light and Enhancement of Skin Anti-Aging Indicators

“…results confirm the composition of Edelweiss cultured as simple callus, but LED was not applied, and, furthermore, EV production was not studied [33].…”

“…There are reports on the simple callus culture and component analysis of Edelweiss, but research on investigating LEDs for the increase in bioactive substances and enhanced EV production is lacking [33]. Our study findings are derived from the observation that the application of specific wavelengths to Panax vietnamensis significantly enhanced callus growth and influenced ginsenoside production, particularly under specific LED conditions [25].…”

Agastache Species (Lamiaceae) as a Valuable Source of Volatile Compounds: GC–MS Profiling and Investigation of In Vitro Antibacterial and Cytotoxic Activities

“…All reagents required for cell culture were purchased from Gibco (Dublin, Ireland). Cell lines included in this study were purchased from American Type Culture Collection (ATCC) and provided by the laboratory biobank [46].…”

“…The absorbance of viable cells was measured at λ = 570 nm using a SPARK10M multiplate reader (Tecan, Männedorf, Switzerland). Each plate contained a blank control (medium alone) and a positive control (DMSO 100%) [46].…”

Active Components of Leontopodium alpinum Callus Culture Extract for Blue Light Damage in Human Foreskin Fibroblasts

“…Nonetheless, it exhibits resistance to severe weather and possesses a significant self-healing ability. Moreover, it showcases distinctive pharmacological properties, including antibacterial [3], antioxidant [4], lipid regulation [5], anti-inflammatory [6], and analgesic effects [7]. However, L. alpinum is now considered a rare species due to its harsh growing environment, growing human demand, and scarce resources.…”