Profiling of secondary metabolites and antimicrobial activity of Crateva religiosa G. Forst. Bark – A rare medicinal plant of Maharashtra India

Abstract

In the present study Profiling of Secondary metabolites and Antimicrobial activity of Stem Bark of Crateva religiosa was carried out for validating the ethno medicinal claims. Stem Bark of this plant was analyzed for Physiochemical study, Organoleptic study and Fluorescent analysis. Qualitative and Quantitative analysis of major secondary metabolites was also carried out using standard procedures. Stem bark was extracted successively using Chloroform, Dichloromethane and 50% Ethanol as solvents which were analyzed by Gas Chromatography -Mass Spectrometry (GC-MS) method to separate and identify the individual compounds in extracts. Antimicrobial activities of all three extracts of understudy part was tested against 4 pathogenic bacterial strains and two fungal strains. The results of antimicrobial activity were compared with the results of standard antibiotics. The physiochemical results determined that percentage of moisture content 6.70 ± 0.59, Ash content 22.18 ± 1.17, Highest Extractive values 15.85 ± 0.21 were found in 50% Ethanol extracts. The qualitative analysis showed presence of various secondary metabolites among which major groups were quantified. By GC-MS analysis the different phyto compounds were identified among which 9 were secondary metabolites and were placed as profile of secondary metabolite in Stem bark. All three solvent extracts showed significant activity against bacterial strains while as chloroform extracts were inactive against fungal strains at 10mg/ml concentration. The results suggest that this plant has vast variety of photochemical which can be used as effective remedy for various ailments and drug formulations in future. The ethnic claims of this plant were also verified by the present study.