Profiling of selected indigenous rice (Oryza sativa L.) landraces of Rarh Bengal in relation to osmotic stress tolerance

Abstract

A total of ten rare indigenous rice landraces of West Bengal were screened for germination potential and seedling growth under varying concentrations of sodium chloride (NaCl) and polyethylene glycol (PEG) solutions as osmotic stress inducing agents. Among the studied rice landraces Kelas and Bhut Moori showed highest degree of tolerance to induced osmotic stresses. Proline content of the studied lines was also determined. Genetic relationship among the studied rice landraces was assessed with 22 previously reported osmotic stress tolerance linked Simple Sequence Repeat (SSR) markers. The identified allelic variants in form of amplified products size (molecular weight) for each SSR marker were documented to find out allele mining set for the linked markers of the studied genotypes in relation to osmotic stress tolerance. A Microsatellite Panel was constructed for the different allelic forms (size of amplified products) of each used marker. Among 22 SSR markers, ten showed unique alleles in form of single specific amplified product for the studied four genotypes which can be used for varietal identification. Genetic relationship among the studied rice lines was determined and a dendrogram was constructed to reveal their genetic inter-relationship. Polymorphism Information Content (PIC) for each used marker was also calculated for the studied rice lines.

Assessment of genetic diversity of Saltol QTL among the rice (Oryza sativa L.) genotypes

“…Several workers also reported that these markers are highly polymorphic (Mohammadi-nejad et al 2008;Karmakar et al 2012;Chattopadhyay et al 2014;Ganie et al 2014). The PCR profile obtained from the SSR markers generated a total of 99 alleles including 20 rare alleles and 16 null alleles, accounting an average of 12.37 alleles/ locus which is higher than the numbers reported by other groups using the SSR markers (Mohammadi-Nejad et al 2008;Islam et al 2012;Karmakar et al 2012;Das et al 2013). Based upon the PIC value, amplicon size range and the number of alleles, RM8094 is advocated to be the most useful and informative marker than others for studying the diversity of Saltol QTL.…”

Improving Grain Yield of Indigenous Rice in Tidal Floodplain of Southern Bangladesh: Effect of Seedling Age and Transplanting Method

“…In our study, low yield was also associated with a fewer number of spikelets per panicle suggesting that major constraining factor in grain yield was the poor sink capacity. Seedling age induced variation in grain yield and associated traits are also related to cultivars [44]. The cultivar that we used in our study had high tillering potential and wide spacing maintained both in row planting and farmers' practice provided enough room for developing tillers and thus despite super sparsely planting [22], the number of tillers and panicles at harvest was comparable to the optimal densities suggested for HYVs [13].…”

Screening Rice Germplasm against Sheath Blight Disease of Rice and its Integrated Management in Bangladesh

“…Consequently, none of the high yielding varieties is resistant to ShB disease neither in Bangladesh nor elsewhere in the world. Fortunately, rice land races have proven to be highly adaptive to diverse environmental conditions and are believed to harbour a number of valuable genetic resources for crop improvement (Karmakar et al, 2012;Roychowdhury et al, 2013;Ganie et al, 2014). Some of the landraces such as Buhjan, Banshpata, Bhasamanik, Nagra Sail, Raghu Sail are tolerant to rice ShB (Dey, 2014).…”