Profiling of seminal plasma proteins to identify the fertility of Simmental bull with low semen quality

Baharun, Abdullah; Rahmi, Annisa; Kardaya, Dede; Said, Syahruddin; Fahrudin, Mokhamad; Arifiantini, Raden; Karja, Ni

doi:10.5455/javar.2023.j689

Cited by 2 publications

(2 citation statements)

References 28 publications

(44 reference statements)

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“…Seminal plasma comprises protein components and other elements like lipids, organic acids, enzymes, and minerals. These constituents play a vital role in spermatozoa metabolism and could influence the fertility of spermatozoa [19] . The expression of proteins in seminal plasma with MWs of 35–50 kDa may negatively affect spermatozoa fertility.…”

Section: Resultsmentioning

confidence: 99%

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Semen quality and frozen semen production in Pasundan bulls: A molecular weight perspective on seminal plasma and spermatozoa protein

Baharun,

Rahmi,

Handarini

et al. 2024

J Adv Vet Anim Res

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Objective: To determine the correlation between the molecular weight (MW) of proteins in seminal plasma and spermatozoa and the quality of fresh and frozen semen production in Pasundan bulls. Materials and methods: Nine selected Pasundan bulls, aged 5–10 years, from the Regional Artificial Insemination Center at Ciamis, West Java, Indonesia, were used in the study, with fresh semen sperm motility ≥70% and <70%. We analyzed the motility, viability, integrity of the intact plasma membrane (IPM), and the morphological characteristics of spermatozoa. 1D-SDS-PAGE analysis was performed to determine the protein profile by assessing MW, depicted as bands on the gel. Results: The motility, viability, and IPM of spermatozoa had lower values (p < 0.05) in Pasundan bulls named Bagaskara and Kertarajasa compared to the other bulls. Proteins with MW 35–50 kDa were not detected in the seminal plasma of Pasundan bulls, exhibiting low quality in fresh semen. The correlation analysis showed that the non-detected proteins with MW 35–50 kDa in seminal plasma correlated with spermatozoa motility (r = 0.421), viability (r = 0.424), and IPM (r = 0.428) so that fresh semen quality was low in both Pasundan bulls. Analysis of semen volume, spermatozoa concentration, and spermatozoa motility showed that the average frozen semen production of Pasundan bulls per ejaculate was 128.73 ± 15.35 straws. Conclusion: Protein analysis based on MW is a predictive indicator for the quality of fresh semen and the production of frozen semen in Pasundan bulls. Evaluation parameters of fresh semen quality by MW analysis can be used to select Pasundan bulls in Indonesia.

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Section: Resultsmentioning

confidence: 99%

“…The expression of proteins in seminal plasma with MWs of 35–50 kDa may negatively affect spermatozoa fertility. Baharun et al [19] reported that protein expression in seminal plasma may either negatively or positively correlate with spermatozoa fertility or spermatozoa quality.…”

Section: Resultsmentioning

confidence: 99%

Semen quality and frozen semen production in Pasundan bulls: A molecular weight perspective on seminal plasma and spermatozoa protein

Baharun,

Rahmi,

Handarini

et al. 2024

J Adv Vet Anim Res

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Comparative developmental competence of in vitro embryos recovered from Bali cattle with normal and poor sperm motility

Hasbi,

Iskandar,

Sonjaya

et al. 2024

Vet World

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Background and Aim: Fertility is crucial for enhancing the efficiency of livestock production, as it directly impacts the reproductive rates. A comprehensive understanding of the relationship between sperm quality and embryo development is key to optimizing reproductive outcomes and improving the quality of livestock. This study analyzed the developmental competence of in vitro embryos recovered from Bali cattle with normal or poor sperm motility. Materials and Methods: Nine bulls with normal fresh semen (NFS) or poor fresh semen (PFS) motility were ejaculated for semen. Semen ejaculates, including volume, motility, and sperm concentration, were evaluated immediately after collection to measure the quality of the fresh semen. Frozen semen was evaluated using computer-assisted semen analysis (CASA) for motility, progressive sperm motility, distance curve path, distance curve linear, distance straight line, average path velocity, curvilinear velocity, linear velocity, straightness (STR), linearity of forward progression (LIN), wobble, and average lateral head displacement (ALH). Bull groups were used to determine in vitro embryo cleavage ability after fertilization of Bali cattle. Ovaries of Bali cattle were collected by slicing, and only cytoplasmic oocytes with compact cumulus cells were used in this study. The oocytes were matured, and in vitro fertilization was performed using fertilization media with a final sperm concentration of 1.5 Ã— 106 spermatozoa/mL. After 48 h, the embryo cleavage ability of the cultured oocytes was evaluated. Results: There were significant differences in motility values between the NFS and PFS groups; however, there were no significant differences in the volume or sperm concentration. There was a significant difference in the LIN value between the groups but no significant differences in other CASA parameters. There were no significant differences in the cleavage rate and morula between the groups, but a positive correlation was observed between the cleavage rate and the morula and between the morula and ALH. A significant negative correlation was observed between the cleavage rate and STR and between the morula and STR; no significant differences were observed for other variables. Conclusion: Despite variations in sperm characteristics, both normal and poor sperm motility demonstrated comparable in vitro embryonic development competence. These findings provide important insights into the fertility potential of Bali bulls, providing valuable information that can enhance selection strategies to improve the quality of livestock production. Keywords: Bali cattle, embryos, in vitro semen quality.

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Profiling of seminal plasma proteins to identify the fertility of Simmental bull with low semen quality

Cited by 2 publications

References 28 publications

Semen quality and frozen semen production in Pasundan bulls: A molecular weight perspective on seminal plasma and spermatozoa protein

Semen quality and frozen semen production in Pasundan bulls: A molecular weight perspective on seminal plasma and spermatozoa protein

Comparative developmental competence of in vitro embryos recovered from Bali cattle with normal and poor sperm motility

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