2017
DOI: 10.7287/peerj.preprints.3073
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Profiling soil microbial communities with next-generation sequencing: the influence of DNA kit selection and technician technical expertise

Abstract: Structure and diversity of microbial communities are an important research topic in biology, since microbes play essential roles in the ecology of various environments.Different DNA isolation protocols can lead to data bias and can affect results of nextgeneration sequencing. To evaluate the impact of protocols for DNA isolation from soil samples and also the influence of individual handling of samples, we compared results obtained by two researchers (R and T) using two different DNA extraction kits: (1) MO BI… Show more

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Cited by 7 publications
(4 citation statements)
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“…Recently developed advanced methodologies, such as next-generation sequencing (NGS), are extremely useful because of the enhanced sequencing depth that can be achieved compared to previous technologies. To date, NGS methods have been applied mostly to describe the human microbiome, but they have also been used to describe a vast array of environmental and agricultural ecologies [14,15]. This technology has also been applied for the investigation of cheese bacterial diversity [16,17].…”
Section: Introductionmentioning
confidence: 99%
“…Recently developed advanced methodologies, such as next-generation sequencing (NGS), are extremely useful because of the enhanced sequencing depth that can be achieved compared to previous technologies. To date, NGS methods have been applied mostly to describe the human microbiome, but they have also been used to describe a vast array of environmental and agricultural ecologies [14,15]. This technology has also been applied for the investigation of cheese bacterial diversity [16,17].…”
Section: Introductionmentioning
confidence: 99%
“…When several bead beating direct extraction methods were used to compare the bacterial communities of sea water, deep sea sediment, soil, mattress dust, the human mouth, and faeces, samples clustered by environment (Marotz, Amir, Humphrey, Gogul, & Knight, 2017). Similarly, when different direct extraction bead beating methods are used on different soils, samples cluster by soil rather than extraction method (Terrat et al, 2012)(Terrat et al, 2015)(Soliman, Yang, Yamazaki, & Jenke-Kodama, 2017).…”
Section: Resultsmentioning
confidence: 99%
“…Soil microbial community diversity in the Arctic has been investigated for several decades, with recent research on microbial community structure and function enabled by advancements in genomics [8]. In particular, nextgeneration sequencing is an important technique in ecology to sequence multiple samples and explore bacterial species diversity, either by targeting the 16S rRNA gene or by directly sequencing genomic DNA or RNA [15,16]. Several studies mainly use chronosequence methods that examine soil structure and development at a linear distance from glacier edges [8,17,18].…”
Section: Introductionmentioning
confidence: 99%