Profiling T cell receptor β‐chain in responders after immunization with recombinant hepatitis B vaccine

Dong, Yan; Yang, Jiezuan; Ji, Zhongkang; Ju, Wang; Lü, Xiaoqing; Huang, Yuanliang; Zhong, Chengli; Li, Lanjuan

doi:10.1002/jgm.3367

Cited by 5 publications

(4 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“… 29 In our previous study, we reported the diversity and distribution characteristics of the IR (TRB) based on the V(CDR3)J of TRBV with small cases, and there are no significant differences between the subjects with and without responding to HepB vaccination. 18 However, the Shannon index based on CDR3 is significantly higher in the HepB vaccine responders compared to that in non-responders. Similarly, the CF100 is significantly lower in responders.…”

Section: Discussionmentioning

confidence: 95%

“…Subsequently, the raw sequence was screened, and the low quality sequences were deleted, the sequencing background was filtered 17 and the international ImMunoGeneTics (IMGT) database ( www.imgt.org ) was used as standard reference sequences and the sequences obtained were compared with the IMGT database in the present study, please refer to our previous report for more detailed steps. 18 …”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Characterization of the TCR β Chain Repertoire in Peripheral Blood from Hepatitis B Vaccine Responders and Non-Responders

Yang¹,

Li²,

Ye³

et al. 2022

JIR

Self Cite

View full text Add to dashboard Cite

Background: Hepatitis B (HepB) vaccination can effectively prevent the prevalence of hepatitis B virus (HBV) infection. However, the incidence of vaccination failure is about 5~10% and the underlying molecular mechanisms are poorly understood. T cells have an essential role in the recipient's immune response to vaccine, which could be elucidated by high-throughput sequencing (HTS) and bioinformatics analysis. Methods: We conducted HTS of the T cell receptor β chain (TRB) complementarity-determining region 3 (CDR3) repertoires in eighteen positive responders (responders) and 10 negative responders (non-responders) who all had HepB vaccination, the repertoire features of BV, BJ and V-J genes and their diversity, respectively, were compared between the positive and negative responders using the Mann-Whitney test. Moreover, the relatively conserved motifs in CDR3 were revealed and compared to those in the other group's report. Results:The diversity of TRB CDR3 and the frequencies of BV27 and BV7-9 are significantly increased for HepB vaccine responders compared to those in non-responders. The motifs of CDR3s in BV27/J1-1, BV27/J2-5, and BV7-9/J2-5, respectively, were most expressed as "NTE", "QETQ", and "GG-Q (E)-ETQ". Moreover, the motif "KLNSPL" was determined in nearly 80% CDR3s in BV27/J1-6 from HepB vaccine responders for the first time. Conclusion: Our results present the comprehensive profiles of TRB CDR3 in the HepB vaccine responders and non-responders after standard vaccination protocol and determine the relatively conservative motifs of CDR3s that may respond to the HepB vaccine. Further results suggest that the profile of TRB repertoire could distinguish the HepB vaccine responders from non-responders and provide a new target for optimizing and improving the efficiency of the HepB vaccine.

show abstract

Section: Discussionmentioning

confidence: 95%

Section: Methodsmentioning

confidence: 99%

Characterization of the TCR β Chain Repertoire in Peripheral Blood from Hepatitis B Vaccine Responders and Non-Responders

Yang¹,

Li²,

Ye³

et al. 2022

JIR

Self Cite

View full text Add to dashboard Cite

show abstract

“…According to current research, the recipient’s response to the hepatitis B vaccine may be influenced by their T-cell immunity. For instance, the edited T-cell receptor-chain variable was linked to the immune response of healthy vaccinees to recombinant hepatitis B surface antigen (rHBsAg) as well as the production of hepatitis B surface antibody (HbsAb) ( 88 ). Circulating follicular helper T cells (cTfh) and subsets were shown to be involved in the immune response to hepatitis B vaccine injection in vitro , and the CXCR3-Tfh cell subset was preferentially activated when HBsAg stimulated PBMCs.…”

Section: T Cells and Measures Of Hbv-mtct Preventionmentioning

confidence: 99%

Effects of hepatitis B virus infection and strategies for preventing mother-to-child transmission on maternal and fetal T-cell immunity

Cao

Zhang

et al. 2023

Front. Immunol.

View full text Add to dashboard Cite

One of the most common routes of chronic hepatitis B virus (HBV) infection is mother-to-child transmission (MTCT). Approximately 6.4 million children under the age of five have chronic HBV infections worldwide. HBV DNA high level, HBeAg positivity, placental barrier failure, and immaturity of the fetal immune are the possible causes of chronic HBV infection. The passive-active immune program for children, which consists of the hepatitis B vaccine and hepatitis B immunoglobulin, and antiviral therapy for pregnant women who have a high HBV DNA load (greater than 2 × 105 IU/ml), are currently two of the most important ways to prevent the transmission of HBV from mother to child. Unfortunately, some infants still have chronic HBV infections. Some studies have also found that some supplementation during pregnancy can increase cytokine levels and then affect the level of HBsAb in infants. For example, IL-4 can mediate the beneficial effect on infants’ HBsAb levels when maternal folic acid supplementation. In addition, new research has indicated that HBV infection in the mother may also be linked to unfavorable outcomes such as gestational diabetes mellitus, intrahepatic cholestasis of pregnancy, and premature rupture of membranes. The changes in the immune environment during pregnancy and the hepatotropic nature of HBV may be the main reasons for the adverse maternal outcomes. It is interesting to note that after delivery, the women who had a chronic HBV infection may spontaneously achieve HBeAg seroconversion and HBsAg seroclearance. The maternal and fetal T-cell immunity in HBV infection is important because adaptive immune responses, especially virus-specific CD8 T-cell responses, are largely responsible for viral clearance and disease pathogenesis during HBV infection. Meanwhile, HBV humoral and T-cell responses are important for the durability of protection after fetal vaccination. This article reviews the literature on immunological characteristics of chronic HBV-infected patients during pregnancy and postpartum, blocking mother-to-child transmissions and related immune mechanisms, hoping to provide new insights for the prevention of HBV MTCT and antiviral intervention during pregnancy and postpartum.

show abstract

“…Previous work has shown that FAS is associated with an increase in levels of markers related to immunity, such as B lymphocyte response markers, innate immune proinflammatory pathways (14) , antigen processing and presentation markers, nuclear factor-k-gene binding signalling pathways, tumor necrosis factor signalling (15) , interleukin-8 (16) and interleukin-4 (IL-4) (17,18) . Previous research has also revealed a positive association between immune markers and hepatitis B vaccine response, including T-cell receptor β-chain variable (19) , NDRG1-expressing myeloid dendritic cell-2, CDKN1C-expressing myeloid dendritic cell-4 (20) , chemokine (C-X-C motif) ligand 12, https://doi.org/10.1017/S000711452200229X Published online by Cambridge University Press Accepted manuscript interleukin-27 (21) , interferon-γ, interleukin-2 (22) , interleukin-17, interleukin-18 (23) and IL-4 (22,24,25) . Among the above immune markers, we noticed that IL-4 is related to both FAS and hepatitis B vaccine response.…”

Section: Introductionmentioning

confidence: 96%

Folic acid supplementation in pregnant women with hepatitis B surface antigen improves infant hepatitis B surface antibody mediated by infant IL-4

Lian

et al. 2022

Br J Nutr

View full text Add to dashboard Cite

Immunoprophylaxis has not completely eliminated hepatitis B virus (HBV) infection due to hyporesponsiveness to hepatitis B vaccine (HepB). We explored the impact of folic acid supplementation (FAS) in pregnant women with positive hepatitis B surface antigen (HBsAg) on their infant hepatitis B surface antibody (anti-HBs) and the mediation effect of infant interleukin-4 (IL-4). We recruited HBsAg-positive mothers and their neonates at baseline. Maternal FAS was obtained via a questionnaire, and neonatal anti-HBs and IL-4 were detected. Follow-up was performed at 11-13 months of age of infants, when anti-HBs and IL-4 were measured. We applied univariate and multivariate analyses. A mediation effect model was performed to explore the mediating role of IL-4. A total of 399 mother–neonate pairs were enrolled and 195 mother–infant pairs were eligible for this analysis. The infant anti-HBs geometric mean concentrations (GMCs) in the maternal FAS group were significnatly higher than those in the no-FAS group [383.8 mIU/ml, 95% confidence interval (CI): 294.2 mIU/ml to 500.7 mIU/ml vs. 217.0 mIU/ml, 95% CI: 147.0 mIU/ml to 320.4 mIU/ml, z=-3.2, P=0.001]. Infants born to women who took folic acid (FA) within the first trimester were more likely to have high anti-HBs titres (adjusted β-value=194.1, P=0.003). The fold change in IL-4 from neonates to infants partially mediated the beneficial influence of maternal FAS on infant anti-HBs (24.7% mediation effect) after adjusting for confounding factors. FAS during the first trimester to HBsAg-positive mothers could facilitate higher anti-HBs levels in infants aged 11-13 months partly by upregulating IL-4 in infants.

show abstract

Profiling T cell receptor β‐chain in responders after immunization with recombinant hepatitis B vaccine

Cited by 5 publications

References 35 publications

Characterization of the TCR β Chain Repertoire in Peripheral Blood from Hepatitis B Vaccine Responders and Non-Responders

Characterization of the TCR β Chain Repertoire in Peripheral Blood from Hepatitis B Vaccine Responders and Non-Responders

Effects of hepatitis B virus infection and strategies for preventing mother-to-child transmission on maternal and fetal T-cell immunity

Folic acid supplementation in pregnant women with hepatitis B surface antigen improves infant hepatitis B surface antibody mediated by infant IL-4

Contact Info

Product

Resources

About