2018
DOI: 10.1186/s12864-018-5155-2
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Profiling the miRNA-mRNA-lncRNA interaction network in MSC osteoblast differentiation induced by (+)-cholesten-3-one

Abstract: BackgroundOur previous study showed that (+)-cholesten-3-one (CN) has the potential to induce the osteoblastic differentiation of mesenchymal stem cells (MSCs). However, the roles of CN in targeting miRNA-mRNA-lncRNA interactions to regulate osteoblast differentiation remain poorly understood.ResultsA total of 77 miRNAs (36 upregulated and 41 downregulated) and 295 lncRNAs (281 upregulated and 14 downregulated) were significantly differentially expressed during CN-induced MSC osteogenic differentiation. Bioinf… Show more

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Cited by 21 publications
(6 citation statements)
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“…Increasing incidence of bone disorders along with ageing populations call for more effective therapies for bone regeneration 19 . LncRNAs are known to affect osteoblast differentiation via a variety of regulatory mechanisms, such as modification of chromatin, binding to transcription factors and competing endogenous mechanisms, as well as other post‐transcriptional mechanisms 20 . For fully clarifying the comprehensive molecular mechanisms mediating the osteogenic differentiation of BMMSCs, we studied the effects of H19 on the osteogenic differentiation of BMMSCs by miR‐149‐mediated regulation of SDF‐1.…”
Section: Discussionmentioning
confidence: 99%
“…Increasing incidence of bone disorders along with ageing populations call for more effective therapies for bone regeneration 19 . LncRNAs are known to affect osteoblast differentiation via a variety of regulatory mechanisms, such as modification of chromatin, binding to transcription factors and competing endogenous mechanisms, as well as other post‐transcriptional mechanisms 20 . For fully clarifying the comprehensive molecular mechanisms mediating the osteogenic differentiation of BMMSCs, we studied the effects of H19 on the osteogenic differentiation of BMMSCs by miR‐149‐mediated regulation of SDF‐1.…”
Section: Discussionmentioning
confidence: 99%
“…Using CRISPR-Cas9-based gene editing techniques to disrupt these two genes, individually or in combination, we were able to show functional implications of Adora2A and Adora3 in PEMF-mediated osteoblast differentiation. We kept our study focused mainly on mRNA expression of differentiation genes because it has been reported in several previous studies that their protein expressions correlated mRNA expression [44][45][46], which also matched our observation in the case of AlpI enzymatic assay (Fig 3). Our results indicate that the A 2A and A 3 receptors are required for at least two parallel and complementary signaling pathways during the differentiation process: A 2A seems to be important in PEMF-mediated stimulation of cFos and Pthr1 pathways while A 3 appears to modulate Alp, BSPI, Ibsp, Osteocalcin and Sp7 pathways (summarized in Fig 7).…”
Section: Plos Onesupporting
confidence: 87%
“…In recent years, ncRNAs, such as lncRNAs and miR-NAs, have emerged as previously underappreciated classes of gene expression modulators that regulate various cellular processes [9][10][11]46]. In the current study, we illustrated a comprehensive ceRNA network for MACF1 deletion to regulate osteoblast proliferation through bioinformatics analysis of gene chip data.…”
Section: Discussionmentioning
confidence: 97%