There are no chemical plant growth retardants that may be used on containerized culinary herbs intended for consumption. Our objective was to quantify the effect of substrate moisture content on the growth of four commonly produced culinary annual herbs grown in containers in the greenhouse. Seedlings of basil (Ocimum basilicum L.), dill (Anethum graveolens L.), parsley (Petroselinum crispum (Mill.) Fuss), and sage (Salvia officinalis L.) were transplanted into 11.4 cm diameter containers filled with commercial soilless substrate comprising (by vol.) 75% sphagnum peat moss and 25% coarse perlite and amended with 3.0 kg·m −3 of controlled-release fertilizer. After the containers were thoroughly irrigated to container capacity, plants were placed into a sensor-controlled irrigation system, which maintained substrate volumetric water content (VWC) at 0.15, 0.28, 0.30, 0.38, or 0.45 m 3 ·m −3 . Chlorophyll fluorescence, photosynthesis, stomatal conductance, and transpiration were measured 27 d after initiating treatments, and the results showed that chlorophyll fluorescence of parsley and photosynthesis of basil increased as substrate VWC increased from 0.15 to 0.45 m 3 ·m −3 ; the remaining parameters for basil, parsley, and sage were unaffected. Additionally, height, width, leaf area, and shoot dry mass of basil, dill, parsley, and sage increased as substrate volumetric water content increased from 0.15 to 0.45 m 3 ·m −3 . Our results show that growth of basil, dill, parsley, and sage can be promoted or inhibited by providing or withholding water, respectively, with no signs of stress or visual damage resulting from reduced substrate volumetric water content. Therefore, restricting irrigation and substrate volumetric water content is an effective nonchemical growth control method for containerized culinary herbs grown in peat-based substrate.Agronomy 2019, 9, 667 2 of 12 basil supplied with 200 mg·L −1 N from a complete, balanced water-soluble fertilizer are 33% larger than plants supplied with 50 mg·L −1 N from the same fertilizer [4]. Additionally, restricting P to 5 mg·L −1 produced basil, dill, parsley, and sage shorter than plants provided with 40 mg·L −1 [5]. While cultivar selection and nutrient management are useful forms of nonchemical growth control, it may be necessary to use multiple nonchemical methods of controlling growth to achieve the degree of control required in the absence of PGRs.Reducing irrigation or substrate volumetric water content (VWC), commonly referred to as "deficit irrigation", is another effective method of controlling containerized plant growth [6][7][8]. The water available for plant uptake increases and growth is promoted as substrate VWC increases and, as such, restricting irrigation and reducing the substrate VWC can diminish turgor pressure and subsequent stem extension and growth [9]. For example, containerized angelonia (Angelonia angustifolia Benth.) and petunia (Petunia × hybrid Vilm.) bedding plant growth is promoted by substrate VWC and, by reducing VWC, compact plants o...