Progenies of allotriploids of Oriental × Asiatic lilies (Lilium) examined by GISH analysis

Barba-González, R.; Silfhout, A.A. van; Visser, Richard G. F.; Ramanna, M.S.; Tuyl, J.M. van

doi:10.1007/s10681-006-9148-x

Cited by 45 publications

(20 citation statements)

References 25 publications

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“…Most successful triploid 9 diploid/tetraploid crosses in Lilium could be explained by the new hypothesis Our 3x 9 2x and 3x 9 4x crosses were successful, as with previous reports in Lilium (Lim et al 2003;Barba-Gonzalez et al 2006;Khan et al 2009;Xie et al 2010). The success of these crosses can be well explained by the hypothesis that five same genomes of endosperm are essential for endosperm development in Lilium.…”

Section: Discussionsupporting

confidence: 88%

“…Allotetraploids, such as LALA, OAOA, OTOT, have been produced by chromosome doubling on F1-distant hybrids with Cochicine or Oryzalin, and allotriploids, e.g., LAA, AOA, LOO and OTO, produced by diploid 9 tetraploid crosses or 2n gametes of F1 hybrids (Karlov et al 1999;Lim et al 2000Lim et al , 2001BarbaGonzalez et al 2004BarbaGonzalez et al , 2005aZhou et al 2008). Some progenies of a few interploid crosses '2x 9 3x', '3x 9 2x' and '3x 9 4x' have also been obtained in LA and OA lilies (Lim et al 2003; Barba-Gonzalez et al 2006;Zhou 2007;Khan et al 2009). In these studies, progenies of 3x 9 2x and 3x 9 4x crosses were mainly analyzed with GISH for their genome composition, intergenomic recombination or introgression between different lily genomes.…”

Section: Introductionmentioning

confidence: 99%

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Analysis of endosperm development of allotriploid × diploid/tetraploid crosses in Lilium

Zhou

2011

Euphytica

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Lilium are Fritillaria-type plants. Triploid lilies, regardless of their male sterility, can be used as female parents to cross with appropriate males, in contrast to other triploid Polygonum-type plants, which are usually seedless. Up to now, however, little attention has been paid to the reason. Here we made allotriploid 9 diploid/tetraploid crosses in Lilium. Endosperm and progenies of LAA 9 AA/AAAA crosses were analyzed for ploidy level and genome composition. The endosperm of some LAA 9 AA/ AAAA ovules developed well but there was little or no development of LAA 9 LL/OO endosperm. The endosperm genome composition of LAA 9 AA, LAA 9 AAAA, LAA 9 LL, LAA 9 OO are theoretically derived as 5A ? 2L, 6A ? 2L, 4A ? 3L, 4A ? 2L ? O, respectively. Genomic in situ hybridization showed that the progenies of LAA 9 AA/ AAAA were aneuploid. Based on the experimental results and analyses, our hypothesis is that five same genomes of endosperms are essential for its development in triploid 9 diploid/tetraploid crosses of Lilium. The hypothesis can explain the success or failure of 3x 9 2x/4x crosses in Lilium and is of importance for lily breeders who need to know the likelihood of success when producing new cultivars of this economically important horticultural crop.

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Section: Discussionsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Analysis of endosperm development of allotriploid × diploid/tetraploid crosses in Lilium

Zhou

2011

Euphytica

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“…In a previous study it was found that in the progenies of 3x ¡ 2x crosses of LongiXorum £ Asiatic and 3x ¡ 2x and reciprocal crosses of Oriental £ Asiatic hybrids lilies, predominantly near diploid (i.e., aneuploid) or diploid progenies occurred indicating that triploid BC1 plants produced both balanced n as well as near haploid (e.g., n + 1 or n + 2 etc.) gametes Barba-Gonzalez et al 2006a). Similarly, present investigation shows that most of the BC2 progenies obtained after 2x ¡ 3x and in reciprocal crosses produced mostly diploid progenies.…”

Section: Discussionmentioning

confidence: 99%

Potential for analytic breeding in allopolyploids: an illustration from Longiflorum × Asiatic hybrid lilies (Lilium)

et al. 2008

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Ploidy level and intergenomic recombination was studied in interspeciWc hybrids between LongiXorum £ Asiatic lilies (LA hybrid) backcross to Asiatic parents in order to assess the possibility for analytic breeding in lily. By backcrossing the diploid (2n = 2x = 24) F1 interspeciWc hybrid between LongiXorum £ Asiatic lilies to Asiatic parents, 104 BC1 progeny plants were produced. Among these, there were 27 diploids, 73 triploids (2n = 2x = 36) and 4 aneuploids (2x ¡ 1, 2x + 2 or 2x + 3). In addition, by backcrossing triploid BC1 (LAA) plants to diploid Asiatic parents in 2x ¡ 3x and reciprocal combinations, 14 diploid BC2 progenies were produced. Genomic in situ hybridization (GISH) was performed to study the intergenomic recombination and karyotype composition. GISH indicated extensive intergenomic recombination among the chromosomes in LA hybrids. A large number of LongiXorum chromosomes were transmitted to the BC1 progenies from LA hybrids. However, very few LongiXorum chromosomes were transmitted from the BC1 triploid (LAA) plants to the BC2 progenies. The occurrence of diploid plants in the BC progenies of LA hybrids has opened the prospects of analytic breeding in lilies. In this approach, the selection of superior genotypes can be carried out at the diploid level and polyploid forms are synthesized from superior diploid parents. The advantages of analytic breeding are evident: (a) a maximum level of heterozygosity can be attained in the synthetic polyploids and (b) introgression can be achieved with a minimum of linkage drag. Based on GISH results the potential application of analytic breeding in lily allopolyploids has been discussed.

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“…This method was successfully applied in plants [Schwarzacher et al, 1989;Snowdon et al, 1997;Shibata and Hizume, 2002;Barba-Gonzalez et al, 2006] and fishes [Zhu and Gui, 2007;Valente et al, 2009]. In amphibians, Bi and Bogart [2006] performed GISH in unisexual salamanders of the genus Ambystoma (Amphibia, Caudata).…”

mentioning

confidence: 99%

Evidence for Integrity of Parental Genomes in the Diploid Hybridogenetic Water Frog <i>Pelophylax esculentus</i> by Genomic in situ Hybridization

Zaleśna

Choleva

Ogielska

et al. 2011

Cytogenet Genome Res

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The Western Palearctic water frogs Pelophylax ridibundus and P. lessonae were identified as parental (sexual) species and P. esculentus as their interspecific, hybridogenetically reproducing hybrid with hemiclonal heredity. We used genomic in situ hybridization (GISH) to identify parental chromosomes of P.lessonae and P.ridibundus in diploid P. esculentus karyotypes (2n = 26). GISH probes were made by fluorochrome labeling of total genomic DNA extracted from the sexual progenitors. The labeled probe from one species was hybridized to chromosomes of P. esculentus in the presence of excess of unlabeled genomic DNA from the other species. Thus, the P. lessonae probe was blocked by P. ridibundus unlabeled DNA, and vice versa. We successfully discriminated each of the 13 respective parental chromosomes in metaphase complements of the hybrids according to species-specific hybridization signals. GISH enabled us to confirm additional differences between parental chromosomes in size (smaller chromosomes belong to P. lessonae) and in the presence of DAPI-positive centromeric heterochromatin (detected in chromosomes of P. ridibundus, but not in P. lessonae). The fact that no visible intergenomic exchanges were found in metaphase chromosomes of diploid P. esculentus provides important information on the genomic integrity of hemiclonal transmission and supports hybridogenesis as a reproductive mode at the chromosome level for the specimens examined.

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Progenies of allotriploids of Oriental × Asiatic lilies (Lilium) examined by GISH analysis

Cited by 45 publications

References 25 publications

Analysis of endosperm development of allotriploid × diploid/tetraploid crosses in Lilium

Analysis of endosperm development of allotriploid × diploid/tetraploid crosses in Lilium

Potential for analytic breeding in allopolyploids: an illustration from Longiflorum × Asiatic hybrid lilies (Lilium)

Evidence for Integrity of Parental Genomes in the Diploid Hybridogenetic Water Frog <i>Pelophylax esculentus</i> by Genomic in situ Hybridization

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